Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

 XU Lingwen , WANG Huabing , JIE Fengying , DENG Shuping , DONG Fang

Department of Critical Care Medicine, Wuhan Third Hospital, Wuhan, Hubei 430060, P. R. China;

Corresponding author：

XU Lingwen, Email: 2848251357@qq.com

Keywords：

Acute lung injury; Lipopolysaccharide; Ophiopogonin D; Th17/Treg cells

DOI：

10.7507/1671-6205.202007004

Video：

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Objective To study the protective effect and mechanism of ophiopogonin D (OP-D) on lipopolysaccharide induced acute lung injury (ALI) in mice.Methods Fifty SPF C57BL/6 mice were randomly divided into five groups, ie. a control group, a sham operation group, a model group, an OP-D group (10 mg·kg^–1·d^–1), and a dexamethasone group (2 mg·kg^–1·d^–1), with 10 mice in each group. One day before the establishment of the model, the OP-D group and the dexamethasone group received the corresponding drugs by gavage. The model group, the OP-D group and the dexamethasone group received lipopolysaccharide (2 mg/kg, 30 μL) through the trachea to establish the ALI model. The sham operation group received the same volume of normal saline. The blank control group was not treated. Six hours after the operation, the mice were weighed and then killed for peripheral blood and lung tissue. The weight of lung tissue was measured to evaluate the degree of pulmonary edema; the pathological changes of lung tissue were observed by hematoxylin-eosin staining; the mRNA expressions of interleukin (IL)-6, IL-10, and IL-17 in lung tissue were detected by qPCR; the percentage of Th17 and Treg cells in peripheral blood was detected by flow cytometry.Results Compared with the model group, the degree of pulmonary edema in the OP-D group decreased significantly (P<0.05), the lung tissue injury decreased, the mRNA expressions of IL-6 and IL-17 in the lung tissue and the proportion of Th17 cells in the peripheral blood decreased significantly (P<0.05), the proportion of Treg cells in the peripheral blood and the mRNA expression of IL-10 in the lung tissue increased significantly (P<0.05).Conclusion OP-D may have therapeutic effect on LPS induced ALI in mice by regulating the balance of Th17/Treg cells.

Citation： XU Lingwen, WANG Huabing, JIE Fengying, DENG Shuping, DONG Fang. Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(7): 503-508. doi: 10.7507/1671-6205.202007004 Copy

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19.	袁林. Th17/Treg 在毛细支气管炎发病机制中的作用. 国际儿科学杂志, 2017, 44(10): 697-700.
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22.	王莹. 麦冬皂苷 D 通过激活 AMPK/NF-κB 信号通路缓解 PM2.5 诱导的肺泡上皮细胞炎症反应[D]. 吉林大学, 2019.

1. 李旭, 陈岩, 白雪松, 等. 中药治疗急性肺损伤/急性呼吸窘迫综合征作用机制研究进展. 辽宁中医药大学学报, 2017, 19(1): 166-170.
2. 伍桂雄, 朱水泉, 董树烽, 等. COPD 急性加重期患者治疗前后血清 IL-17 Th17 Treg 细胞变化及其与肺功能的相关性研究. 河北医学, 2020, 26(1): 13-18.
3. 秦翌佳, 老启芳, 黄冰, 等. 白细胞介素-17 对脂多糖致急性肺损伤小鼠炎症免疫调节的影响. 中华危重病急救医学, 2019, 31(8): 983-988.
4. 宋丽娟, 朱煜, 金鸣. 羟基红花黄色素 A 抑制脂多糖诱导的急性肺损伤小鼠炎症因子表达的研究. 中国呼吸与危重监护杂志, 2016, 15(6): 577-582.
5. 喻敏. 沙参麦冬汤加减应用于慢性支气管炎的效果及对患者血清肺表面活性蛋白的影响. 临床医学研究与实践, 2018, 3(17): 114-115.
6. 陈哲, 冯佳悦, 曹诚, 等. 襄麦冬多糖及皂苷体外活性研究. 食品研究与开发, 2018, 39(13): 7-12.
7. 王佳, 王宁宁, 葛运炫, 等. 麦冬皂苷 D 干预内质网应激减轻麦冬皂苷 D’所致心肌细胞损伤. 中国中药杂志, 2019, 44(9): 1876-1881.
8. Ma C, Long H, Yang C, et al. Anti-inflammatory role of pilose antler peptide in LPS-induced lung injury. Inflammation, 2017, 40(3): 904-912.
9. Zhang YY, Meng C, Zhang XM, et al. Ophiopogonin D attenuates doxorubicin-induced autophagic cell death by relieving mitochondrial damage in vitro and in vivo. J Pharmacol Exp Ther, 2014, 352(1): 166-174.
10. 吕涛, 李延仓, 李树仁, 等. 依达拉奉对脂多糖引起的小鼠急性肺损伤的保护效应研究. 大连医科大学学报, 2012, 34(4): 343-347.
11. 程超. 麦冬果实蓝色素的抗氧化特性研究. 中国酿造, 2009, 205(4): 62-64.
12. 王佳佳, 卢宗亮, 孔亚, 等. 麦冬皂苷 D’通过 RIP1/MLKL 诱导前列腺癌 PC3 细胞程序性坏死. 第三军医大学学报, 2017, 39(3): 201-207.
13. Song J, Suh YJ, Lee HJ, et al. Flecainide improve sepsis induced acute lung injury by controlling inflammatory response. Crit Care Med, 2016, 31(3): 194-201.
14. Knochelmann HM, Dwyer CJ, Bailey SR, et al. When worlds collide: Th17 and Treg cells in cancer and autoimmunity. Cell Mol Immunol, 2018, 15(5): 458-469.
15. Cheng L, Zhao Y, Qi D, et al. Wnt/β-catenin pathway promotes acute lung injury induced by LPS through driving the Th17 response in mice. Biochem Biophys Res Commun, 2018, 495(2): 1890-1895.
16. 齐欣欣, 应旭旻. 中医药对 Treg 细胞和 Th17 细胞分化的研究进展. 浙江中西医结合杂志, 2016, 26(3): 298-301.
17. Hirahara K, Nakayama T. CD4⁺ T-cell subsets in inflammatory diseases: beyond the T_h1/T_h2 paradigm. Int Immunol, 2016, 28(4): 163-171.
18. 张海萍, 李芳. 调节性 T 细胞分化和功能调节机制的研究进展. 免疫学杂志, 2019, 35(12): 1100-1104.
19. 袁林. Th17/Treg 在毛细支气管炎发病机制中的作用. 国际儿科学杂志, 2017, 44(10): 697-700.
20. Zhu J. The research of Treg cells: progress and challenge. World J Cardiovascr Dis, 2015, 5(6): 150-165.
21. 庄文珺, 谢炎, 顾剑玲, 等. 调节性 T 细胞在间接性急性肺损伤小鼠中的作用与机制. 中华结核和呼吸杂志, 2018, 41(12): 959-966.
22. 王莹. 麦冬皂苷 D 通过激活 AMPK/NF-κB 信号通路缓解 PM2.5 诱导的肺泡上皮细胞炎症反应[D]. 吉林大学, 2019.

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Protective effect of ophiopogon japonicus saponin D on lipopolysaccharide induced acute lung injury in mice

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