阿托伐他汀钙对香烟暴露大鼠肺血管炎症模型组蛋白脱乙酰酶2的影响_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

何永鸿 ¹ ,  王宋平 ²

1. ;
2. ;

Corresponding author：

王宋平, Email: 1808264282@qq.com

Keywords：

阿托伐他汀钙; 蛋白脱乙酰酶2; 香烟暴露; 大鼠; 肺血管炎症

DOI：

10.7507/1671-6205.202007090

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的探讨阿托伐他汀钙对香烟暴露大鼠肺血管炎症的作用及对肺组织、血清中组蛋白脱乙酰酶2（HADC2）的影响。方法将18只雌性SD大鼠随机分为空白组（A组）、肺血管模型组（B组）、阿托伐他汀钙干预组（C组），每组6只，使用烟熏加气道滴入脂多糖经典造模方法对B组和C组大鼠进行香烟暴露肺血管炎症模型制作，C组于第2天开始，每天熏烟前1 h用阿托伐他汀片灌胃治疗，剂量5 mg/（kg·d），A、B组同期予以等量生理盐水进行灌胃，35 d后处死大鼠。使用苏木精–伊红染色对大鼠各组病理组织进行染色，并对血管的炎症进行评分。酶联免疫吸附试验检测血清中HDAC2水平，蛋白印迹法检查肺组织中HDAC2蛋白水平，实时荧光定量聚合酶链反应检测肺组织HDAC2 mRNA水平。结果与A组比较，B、C组的肺血管炎症明显，但C组轻于B组。A组炎症评分为（0.5±0.5）分，略低于C组［（1.7±0.7）分］，显著低于B组［（2.7±1.0）分］，C组炎症评分显著低于B组。A组大鼠血清中HADC2水平为（12.76±0.63）ng/mL，略高于C组［（11.59±0.60）ng/mL］，但C、A组明显高于B组［（2.27±0.42）ng/mL］，三组之间的差异有统计学意义（P<0.05）。B组大鼠肺组织中HDAC2蛋白表达明显减少，而通过阿托伐他汀钙干预后，C组的HDAC2表达（0.30±0.02）相较于B组（0.09±0.01）明显增加，略低于A组（0.35±0.04）；A组（1.23±0.06）、C组（0.82±0.03）的HDAC2 mRNA水平明显高于B组（0.27±0.02），三组之间的差异有统计学意义（P<0.05）。结论阿托伐他汀钙可减轻香烟暴露大鼠肺血管炎症的程度，其机制可能与增加HDAC2的表达有关。

Citation： 何永鸿, 王宋平. 阿托伐他汀钙对香烟暴露大鼠肺血管炎症模型组蛋白脱乙酰酶2的影响. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(12): 881-884. doi: 10.7507/1671-6205.202007090 Copy

1.	Bourbeau J, Celli BR, Criner GJ, et al. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease: the GOLD science committee report 2019. Eur Respir J, 2019, 53(5): 1900164.
2.	McLaughlin VV, Shah SJ, Souza R, et al. Management of pulmonary arterial hypertension. J Am Coll Cardiol. 2015, 65(18): 1976-1997.
3.	Celebi S, Celebi OO, Aydogdu S. Beyond lipids: antiarrhythmic effects of statins. Angiology, 2019, 70(3): 283.
4.	Howard ML, Vincent AH. Statin effects on exacerbation rates, mortality, and inflammatory markers in patients with chronic obstructive pulmonary disease: a review of prospective studies. Pharmacotherapy, 2016, 36(5): 536-547.
5.	Lu Y, Chang R, Yao J, et al. Effectiveness of long-term using statins in COPD - a network meta-analysis. Respir Res, 2019, 20(1): 17.
6.	Chen F, Yang M, Wan C, et al. Efficacy and safety of statin therapy in pulmonary hypertension: a systematic review and meta-analysis. Ann Transl Med, 2019, 7(23): 786.
7.	Pathak NN, Lingaraju MC, Balaganur V, et al. Anti-inflammatory and chondroprotective effects of atorvastatin in a cartilage explant model of osteoarthritis. Inflamm Res, 2015, 64(3-4): 161-169.
8.	樊梅, 邓俊, 王宋平. 阿奇霉素对慢性阻塞性肺疾病大鼠肺血管重构的影响. 中国呼吸与危重监护杂志, 2018, 17(2): 128-133.
9.	Zhou Y, Tan X, Kuang W, et al. Erythromycin ameliorates cigarette-smoke-induced emphysema and inflammation in rats. Transl Res, 2012, 159(6): 464-472.
10.	曹路, 高宝安, 官莉, 等. 慢阻肺患者血清中组蛋白去乙酰化酶2 IL-1βIL-6及IL-17 A的水平及临床意义. 河北医学, 2019, 25(10): 1585-1588.
11.	Lai T, Wu M, Zhang C, et al. HDAC2 attenuates airway inflammation by suppressing IL-17 A production in HDM-challenged mice. Am J Physiol Lung Cell Mol Physiol, 2019, 316(1): L269-L279.
12.	Khangura RK, Bali A, Jaggi AS, et al. Histone acetylation and histone deacetylation in neuropathic pain: An unresolved puzzle?. Eur J Pharmacol, 2017, 795: 36-42.
13.	李珊, 刘永哲, 黄慧, 等. 组蛋白去乙酰化酶在特发性肺纤维化和隐源性机化性肺炎发病机制中的作用. 中华结核和呼吸杂志, 2019, 42(5): 361-366.
14.	Bhavsar P, Ahmad T, Adcock IM. The role of histone deacetylases in asthma and allergic diseases. J Allergy Clin Immunol, 2008, 121(3): 580-584.
15.	Lee CS, Yi EH, Lee JK, et al. Simvastatin suppresses RANTES-mediated neutrophilia in polyinosinic-polycytidylic acid-induced pneumonia. Eur Respir J, 2013, 41(5): 1147-1156.

1. Bourbeau J, Celli BR, Criner GJ, et al. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease: the GOLD science committee report 2019. Eur Respir J, 2019, 53(5): 1900164.
2. McLaughlin VV, Shah SJ, Souza R, et al. Management of pulmonary arterial hypertension. J Am Coll Cardiol. 2015, 65(18): 1976-1997.
3. Celebi S, Celebi OO, Aydogdu S. Beyond lipids: antiarrhythmic effects of statins. Angiology, 2019, 70(3): 283.
4. Howard ML, Vincent AH. Statin effects on exacerbation rates, mortality, and inflammatory markers in patients with chronic obstructive pulmonary disease: a review of prospective studies. Pharmacotherapy, 2016, 36(5): 536-547.
5. Lu Y, Chang R, Yao J, et al. Effectiveness of long-term using statins in COPD - a network meta-analysis. Respir Res, 2019, 20(1): 17.
6. Chen F, Yang M, Wan C, et al. Efficacy and safety of statin therapy in pulmonary hypertension: a systematic review and meta-analysis. Ann Transl Med, 2019, 7(23): 786.
7. Pathak NN, Lingaraju MC, Balaganur V, et al. Anti-inflammatory and chondroprotective effects of atorvastatin in a cartilage explant model of osteoarthritis. Inflamm Res, 2015, 64(3-4): 161-169.
8. 樊梅, 邓俊, 王宋平. 阿奇霉素对慢性阻塞性肺疾病大鼠肺血管重构的影响. 中国呼吸与危重监护杂志, 2018, 17(2): 128-133.
9. Zhou Y, Tan X, Kuang W, et al. Erythromycin ameliorates cigarette-smoke-induced emphysema and inflammation in rats. Transl Res, 2012, 159(6): 464-472.
10. 曹路, 高宝安, 官莉, 等. 慢阻肺患者血清中组蛋白去乙酰化酶2 IL-1βIL-6及IL-17 A的水平及临床意义. 河北医学, 2019, 25(10): 1585-1588.
11. Lai T, Wu M, Zhang C, et al. HDAC2 attenuates airway inflammation by suppressing IL-17 A production in HDM-challenged mice. Am J Physiol Lung Cell Mol Physiol, 2019, 316(1): L269-L279.
12. Khangura RK, Bali A, Jaggi AS, et al. Histone acetylation and histone deacetylation in neuropathic pain: An unresolved puzzle?. Eur J Pharmacol, 2017, 795: 36-42.
13. 李珊, 刘永哲, 黄慧, 等. 组蛋白去乙酰化酶在特发性肺纤维化和隐源性机化性肺炎发病机制中的作用. 中华结核和呼吸杂志, 2019, 42(5): 361-366.
14. Bhavsar P, Ahmad T, Adcock IM. The role of histone deacetylases in asthma and allergic diseases. J Allergy Clin Immunol, 2008, 121(3): 580-584.
15. Lee CS, Yi EH, Lee JK, et al. Simvastatin suppresses RANTES-mediated neutrophilia in polyinosinic-polycytidylic acid-induced pneumonia. Eur Respir J, 2013, 41(5): 1147-1156.

Previous Article
肺放线菌病一例报告
Next Article
八例星座链球菌致脓胸临床分析

Chinese Journal of Respiratory and Critical Care Medicine

阿托伐他汀钙对香烟暴露大鼠肺血管炎症模型组蛋白脱乙酰酶2的影响

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content