Effects of particulate matter exposure on heart and lung tissues of rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Nian ¹ , ZHENG Yufan ² , HUANG Shaojian ³ , GU Ganbin ⁴ , XU Yang ⁴ ,  HE Fang ¹

1. School of Basic Medical Science, Guangzhou Medical University, Guangzhou, Guangdong 511436, P. R. China;
2. School of Public Health, Guangdong Medical University, Dongguan, Guangdong 523000, P. R. China;
3. School of Clinical Medicine, Guangzhou Medical University, Guangzhou, Guangdong 511436, P. R. China;
4. College of Life Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, P. R. China;

Corresponding author：

HE Fang, Email: 942335012@qq.com

Keywords：

Particulate matter; Motor vehicle exhaust; Biomass fuel; Rat; Heart; Lung

DOI：

10.7507/1671-6205.202105006

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To observe the effects of atmospheric particulate matter (PM) exposure from two sources of motor vehicle exhaust (MVE) and biomass fuel (BMF) on the heart and lung tissues of rats. Methods Thirty rats were randomly divided into 3 groups, and they were exposed to clean air (control group), MVE (MVE group) and BMF (BMF group) for 12 weeks, respectively. The effect of long-term PM exposure on the morphological structure of heart and lung tissues was investigated by tissue and cell morphological observations, Masson staining, and immunohistochemistry. Results Compared with rats in the control group, the rats in the MVE group and the BMF group showed cellular degeneration, increased number of interstitial inflammatory cells, fibrous tissue proliferation, and cell apoptosis in the heart and lung tissues of the PM-exposed rats. Conclusion Exposure to air pollution particulate matter for 12 weeks can significantly cause inflammatory and damage to the heart and lung tissues of rats.

Citation： WANG Nian, ZHENG Yufan, HUANG Shaojian, GU Ganbin, XU Yang, HE Fang. Effects of particulate matter exposure on heart and lung tissues of rats. Chinese Journal of Respiratory and Critical Care Medicine, 2021, 20(11): 795-801. doi: 10.7507/1671-6205.202105006 Copy

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8.	He F, Liao B, Pu J, et al. Exposure to ambient particulate matter induced COPD in a rat model and a description of the underlying mechanism. Sci Rep, 2017, 7: 45666.
9.	Pope CA, Bhatnagar A, McCracken, et al. Exposure to fine particulate air pollution is associated with endothelial injury and systemic inflammation. Circ Res, 2016, 119(11): 1204-1214.
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15.	Huang L, Pu J, He F, et al. Positive feedback of the amphiregulin-EGFR-ERK pathway mediates PM2. 5 from wood smoke-induced MUC5 AC expression in epithelial cells. Sci Rep, 2017, 7(1): 11084.
16.	Ye XQ, Hong W, Hao BW, et al. PM2.5 promotes human bronchial smooth muscle cell migration via the Sonic hedgehog signaling pathway. Respir Res, 2018, 19(1): 37.
17.	蒲金定, 黄玲媚, 叶秀琴, 等. 环境中细微颗粒物对人支气管上皮细胞转分化的影响. 中华结核和呼吸杂志, 2016, 39(10): 784-790.
18.	Cheng PY, Li SY, Chen HY. Macrophages in lung Injury, repair, and fibrosis. Cells, 2021, 10(2): 436.
19.	Liu JY, Liang S, Du Z, et al. PM 2.5 aggravates the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells. Environ Pollut, 2019, 249: 482-490.
20.	Demedts IK, Demoor T, Bracke KR, et al. Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema. Respir Res, 2006, 7(1): 53.
21.	Zhou TY, Hu Y, Wang YX, et al. Fine particulate matter (PM 2.5) aggravates apoptosis of cigarette-inflamed bronchial epithelium in vivo and vitro. Environ Pollut, 2019, 248: 1-9.
22.	Lee YG, Lee PH, Choi SM, et al. Effects of air pollutants on airway diseases. Int J Environ Res Public Health, 2021, 18(18): 9905.
23.	Rajagopalan S, Brauer M, Bhatnagar A, et al. Personal-Level Protective Actions Against Particulate Matter Air Pollution Exposure: A Scientific Statement From the American Heart Association. Circulation, 2020, 142(23): e411-e431.
24.	Ju S, Lim L, Jiao HY, et al. Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes. Part Fibre Toxicol, 2020, 17(1): 25.
25.	Qi Z, Song Y, Ding Q, et al. Water soluble and insoluble components of PM2.5 and their functional cardiotoxicities on neonatal rat cardiomyocytes in vitro. Ecotoxicol Environ Saf, 2019, 168: 378-387.
26.	Smolgovsky S, Ibeh U, Tamayo TP, et al. Adding insult to injury - Inflammation at the heart of cardiac fibrosis. Cell Signal, 2021, 77: 109828.
27.	Del Re DP, Amgalan D, Linkermann A, et al. Fundamental mechanisms of regulated cell death and implications for heart disease. Physiol Rev, 2019, 99(4): 1765-1817.

1. Guan WJ, Zheng XY, Chung KF, et al. Impact of air pollution on the burden of chronic respiratory diseases in China: time for urgent action. Lancet, 2016, 388(10054): 1939-1951.
2. Jalaludin B, Cowie C. Particulate air pollution and cardiovascular disease-it is time to take it seriously. Rev Environ Health, 2014, 29(1-2): 129-132.
3. Misiukiewicz-Stepien P, Paplinska-Goryca M. Biological effect of PM 10 on airway epithelium-focus on obstructive lung diseases. Clin Immunol, 2021, 227: 108754.
4. Lelieveld J, Münzel T. Air pollution, the underestimated cardiovascular risk factor. Eur Heart J, 2020, 41: 904-905.
5. Alexeeff SE, Liao NS, Liu X, et al. Long-term PM2.5 exposure and risks of ischemic heart disease and stroke events: review and meta-analysis. J Am Heart Assoc, 2021, 10(1): e016890.
6. Yue H, He C, Huang Q, et al. Stronger policy required to substantially reduce deaths from PM2. 5 pollution in China. Nat Commun, 2020, 11(1): 1462.
7. Li N, Yang Z, Liao B, et al. Chronic exposure to ambient particulate matter induces gut microbial dysbiosis in a rat COPD model. Respir Res, 2020, 21(1): 271.
8. He F, Liao B, Pu J, et al. Exposure to ambient particulate matter induced COPD in a rat model and a description of the underlying mechanism. Sci Rep, 2017, 7: 45666.
9. Pope CA, Bhatnagar A, McCracken, et al. Exposure to fine particulate air pollution is associated with endothelial injury and systemic inflammation. Circ Res, 2016, 119(11): 1204-1214.
10. Zhou Y, Zou Y, Li X, et al. Lung function and incidence of chronic obstructive pulmonary disease after improved cooking fuels and kitchen ventilation: a 9-year prospective cohort study. PLoS Med, 2014, 11(3): e1001621.
11. Li J, Liu F, Liang F, et al. Long-term effects of high exposure to ambient fine particulate matter on coronary heart disease incidence: a population-based Chinese cohort study. Environ Sci Technol, 2020, 54(11): 6812-6821.
12. Li J, Lu X, Liu F, et al. Chronic effects of high fine particulate matter exposure on lung cancer in P. R. China. Am J Respir Crit Care Med, 2020, 202(11): 1551-1559.
13. Kopf M, Schneider C, Nobs SP. The development and function of lung-resident macrophages and dendritic cells. Nat Immunol, 2015, 16(1): 36-44.
14. Sussan TE, Ingole V, Kim JH, et al. Source of biomass cooking fuel determines pulmonary response to household air pollution. Am J Respir Cell Mol Biol, 2014, 50(3): 538-548.
15. Huang L, Pu J, He F, et al. Positive feedback of the amphiregulin-EGFR-ERK pathway mediates PM2. 5 from wood smoke-induced MUC5 AC expression in epithelial cells. Sci Rep, 2017, 7(1): 11084.
16. Ye XQ, Hong W, Hao BW, et al. PM2.5 promotes human bronchial smooth muscle cell migration via the Sonic hedgehog signaling pathway. Respir Res, 2018, 19(1): 37.
17. 蒲金定, 黄玲媚, 叶秀琴, 等. 环境中细微颗粒物对人支气管上皮细胞转分化的影响. 中华结核和呼吸杂志, 2016, 39(10): 784-790.
18. Cheng PY, Li SY, Chen HY. Macrophages in lung Injury, repair, and fibrosis. Cells, 2021, 10(2): 436.
19. Liu JY, Liang S, Du Z, et al. PM 2.5 aggravates the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells. Environ Pollut, 2019, 249: 482-490.
20. Demedts IK, Demoor T, Bracke KR, et al. Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema. Respir Res, 2006, 7(1): 53.
21. Zhou TY, Hu Y, Wang YX, et al. Fine particulate matter (PM 2.5) aggravates apoptosis of cigarette-inflamed bronchial epithelium in vivo and vitro. Environ Pollut, 2019, 248: 1-9.
22. Lee YG, Lee PH, Choi SM, et al. Effects of air pollutants on airway diseases. Int J Environ Res Public Health, 2021, 18(18): 9905.
23. Rajagopalan S, Brauer M, Bhatnagar A, et al. Personal-Level Protective Actions Against Particulate Matter Air Pollution Exposure: A Scientific Statement From the American Heart Association. Circulation, 2020, 142(23): e411-e431.
24. Ju S, Lim L, Jiao HY, et al. Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes. Part Fibre Toxicol, 2020, 17(1): 25.
25. Qi Z, Song Y, Ding Q, et al. Water soluble and insoluble components of PM2.5 and their functional cardiotoxicities on neonatal rat cardiomyocytes in vitro. Ecotoxicol Environ Saf, 2019, 168: 378-387.
26. Smolgovsky S, Ibeh U, Tamayo TP, et al. Adding insult to injury - Inflammation at the heart of cardiac fibrosis. Cell Signal, 2021, 77: 109828.
27. Del Re DP, Amgalan D, Linkermann A, et al. Fundamental mechanisms of regulated cell death and implications for heart disease. Physiol Rev, 2019, 99(4): 1765-1817.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of particulate matter exposure on heart and lung tissues of rats

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