Anti-tumor effect of superantigen staphylococcal enterotoxin B gene for mice beared by Lewis lung cancer cell_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Xiaoyong ¹ , FENG Min ² ,  TANG Xiaojun ³

1. Department of Pediatric Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;
2. Department of Cardiovascular Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;
3. Department of Thoracic Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

TANG Xiaojun, Email: tangxiaojun73@163.com

Keywords：

Lung cancer; superantigen; staphylococcus enterotoxin B; immunogene therapy

DOI：

10.7507/1671-6205.202112015

Video：

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Objective To explore the tumor suppressive effect of superantigen staphylococcal enterotoxin B (SEB) for mice beared by Lewis lung cancer cell (LLCC). Methods SEB gene expressive plasmid PCDH-SEB-GFP was constructed and transfected into LLCC and it was detected by Western blot. Tumor-bearing mice model was established by subcutaneous injection of LLCC and SEB expressive plasmid PCDH-SEB-GFP was successfully injected into the tumor to observe its suppressive effect on the growth of Lewis lung cancer in mice. Results SEB expression was detected after transfection of LLCC with SEB gene plasmid PCDH-SEB-GFP. Intratumoral injection of plasmid PCDH-SEB-GFP could significantly inhibit the growth of Lewis lung cancer in tumor-bearing mice. Conclusions Intratumoral injection of superantigen SEB expressive plasmid can inhibit the growth of Lewis lung cancer in mice. It deserves a further study that SEB gene can work as an exogenous antigen gene in immune gene therapy for lung cancer.

Citation： WANG Xiaoyong, FENG Min, TANG Xiaojun. Anti-tumor effect of superantigen staphylococcal enterotoxin B gene for mice beared by Lewis lung cancer cell. Chinese Journal of Respiratory and Critical Care Medicine, 2022, 21(3): 195-199. doi: 10.7507/1671-6205.202112015 Copy

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8.	Krakauer T. Update on staphylococcal superantigen-induced signaling pathways and therapeutic interventions. Toxins (Basel), 2013, 5(9): 1629-1654.
9.	Krakauer T, Pradhan K, Stiles BG. Staphylococcal superantigens spark host-mediated danger signals. Front Immunol, 2016, 7: 23.
10.	Shivaee A, Sedighi M, Imani FAA. Staphylococcal enterotoxins as good candidates for cancer immunotherapy: a systematic review. Ann Ig, 2020, 32(6): 648-663.
11.	Terman DS, Bohach G, Vandenesch F, et al. Staphylococcal superantigens of the enterotoxin gene cluster (egc) for treatment of stage IIIb non-small cell lung cancer with pleural effusion. Clin Chest Med, 2006, 27(2): 321-334.
12.	Maleki F, Sadeghifard N, Sedighian H, et al. TGFαL3-SEB fusion protein as an anticancer against ovarian cancer. Eur J Pharmacol, 2020, 870: 172919.
13.	Ejtehadifar M, Halabian R, Fooladi AAI, et al. Anti-cancer effects of staphylococcal enterotoxin type B on U266 cells co-cultured with mesenchymal stem cells. Microb Pathog, 2017, 113: 438-444.
14.	Krakauer T, Stiles BG. The staphylococcal enterotoxin (SE) family: SEB and siblings. Virulence, 2013, 4(8): 759-773.
15.	李飞, 周帅, 董慧, 等. 金黄色葡萄球菌肠毒素B基因在大肠杆菌中的克隆及表达. 检验医学与临床, 2016, 13(18): 2553-2555.
16.	朱大冕, 陈全, 李奕璇, 等. 肿瘤特异的sea基因真核表达质粒的构建及在肺癌细胞中的功能研究. 生物技术通报, 2009, (5): 109-112.
17.	Re F, Srinivasan R, Igarashi T, et al. Green fluorescent protein expression in dendritic cells enhances their immunogenicity and elicits specific cytotoxic T-cell responses in humans. Exp Hematol, 2004, 32(2): 210-217.
18.	Ansari AM, Ahmed AK, Matsangos AE, et al. Cellular GFP toxicity and immunogenicity: potential confounders in in vivo cell tracking experiments. Stem Cell Rev Rep, 2016, 12(5): 553-559.
19.	Fooladi AA, Sattari M, Hassan ZM, et al. In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin. Biotechnol Lett, 2008, 30(12): 2053-2059.

1. Torre LA, Siegel RL, Jemal A. Lung cancer statistics. Adv Exp Med Biol, 2016, 893: 1-19.
2. 邹小农, 贾漫漫, 王鑫, 等. 中国肺癌和烟草流行及控烟现状. 中国肺癌杂志, 2017, 20(8): 505-510.
3. Hirsch FR, Scagliotti GV, Mulshine JL, et al. Lung cancer: current therapies and new targeted treatments. Lancet, 2017, 389: 299-311.
4. Krakauer T. Staphylococcal superantigens: pyrogenic toxins induce toxic shock. Toxins (Basel), 2019, 11(3): 178.
5. Marrack P, Kappler J. The staphylococcal enterotoxins and their relatives. Science, 1990, 248(4956): 705-711.
6. 王小勇, 廖斌, 于海清, 等. 肿瘤特异性结核抗原Ag85A基因慢病毒表达载体构建. 中国胸心血管外科临床杂志, 2015, 22(11): 1050-1055.
7. Kotzin BL, Leung DYM, Kappler J, et al. Superantigens and their potential role in human disease. Adv Immunol, 1993, 54: 99-166.
8. Krakauer T. Update on staphylococcal superantigen-induced signaling pathways and therapeutic interventions. Toxins (Basel), 2013, 5(9): 1629-1654.
9. Krakauer T, Pradhan K, Stiles BG. Staphylococcal superantigens spark host-mediated danger signals. Front Immunol, 2016, 7: 23.
10. Shivaee A, Sedighi M, Imani FAA. Staphylococcal enterotoxins as good candidates for cancer immunotherapy: a systematic review. Ann Ig, 2020, 32(6): 648-663.
11. Terman DS, Bohach G, Vandenesch F, et al. Staphylococcal superantigens of the enterotoxin gene cluster (egc) for treatment of stage IIIb non-small cell lung cancer with pleural effusion. Clin Chest Med, 2006, 27(2): 321-334.
12. Maleki F, Sadeghifard N, Sedighian H, et al. TGFαL3-SEB fusion protein as an anticancer against ovarian cancer. Eur J Pharmacol, 2020, 870: 172919.
13. Ejtehadifar M, Halabian R, Fooladi AAI, et al. Anti-cancer effects of staphylococcal enterotoxin type B on U266 cells co-cultured with mesenchymal stem cells. Microb Pathog, 2017, 113: 438-444.
14. Krakauer T, Stiles BG. The staphylococcal enterotoxin (SE) family: SEB and siblings. Virulence, 2013, 4(8): 759-773.
15. 李飞, 周帅, 董慧, 等. 金黄色葡萄球菌肠毒素B基因在大肠杆菌中的克隆及表达. 检验医学与临床, 2016, 13(18): 2553-2555.
16. 朱大冕, 陈全, 李奕璇, 等. 肿瘤特异的sea基因真核表达质粒的构建及在肺癌细胞中的功能研究. 生物技术通报, 2009, (5): 109-112.
17. Re F, Srinivasan R, Igarashi T, et al. Green fluorescent protein expression in dendritic cells enhances their immunogenicity and elicits specific cytotoxic T-cell responses in humans. Exp Hematol, 2004, 32(2): 210-217.
18. Ansari AM, Ahmed AK, Matsangos AE, et al. Cellular GFP toxicity and immunogenicity: potential confounders in in vivo cell tracking experiments. Stem Cell Rev Rep, 2016, 12(5): 553-559.
19. Fooladi AA, Sattari M, Hassan ZM, et al. In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin. Biotechnol Lett, 2008, 30(12): 2053-2059.

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Anti-tumor effect of superantigen staphylococcal enterotoxin B gene for mice beared by Lewis lung cancer cell

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