Efficacy of electric conduction on rats with penicillin-induced neocortical epilepsy_Journal of Epilepsy

Authors：

 ZHANGShaohui ,  LIANGShuli , YUXiaoman , CHAIXubin , ZHANGLimin , LIANGShuangshuang

Department of Neurosurgery, First Affiliated Hospital of PLA General Hospital. Beijing 100048, China;

Corresponding author：

LIANGShuli, Email: liangsl_304@sina.cn

Keywords：

Neocortical epilepsy; Electric conduction; Therapy; Rats

DOI：

10.7507/2096-0247.20160008

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To confirm that conduction of the epileptiform discharge activity out of the cranium can by reduce the seizure and consequence neuron injury, and introduce the novel micro invasive neurosurgical approach to epileptic therapy. Methods To build penicillin-induced neocortical epilepsy rat (n=60, 4 groups, 15 per group, including control group, conducting group, pseudo-conduction group and model control group). The specialized self-made conducting electrode was used to building conducting epileptic rat model. The epileptiform discharges were recorded by EEG with deep needle electrode for 2 hours under anesthesia, and seizures were monitored by video for 48 hours in waking. At last, the apoptosis ratio of neocortex was tested with flow cytometer. Results We built 41 (91.1%) penicillin-induced neocortical epilepsy rats successfully. The mean frequency of total epileptiform discharges and frequency of diffused epileptiform discharges in EEG in conducting group were significantly less than the numbers in model control group and pseudo-conduction group(P < 0.01). However, significant difference was not found in times of focal epileptiform discharges among 3 test groups. During video monitoring, significant difference presented in the frequency of clinical seizure between conducting group and model control group or pseudo-conducting group. Furthermore, apoptosis ratios of neuron in conducting group was significantly less than the other two groups (P < 0.001). Conclusions Conducting the epileptiform discharge activity out of the cranium can prevent the seizure and reduce epileptiform discharge and apoptosis ratio of neocortex in neocortical epilepsy rats.

Citation： ZHANGShaohui, LIANGShuli, YUXiaoman, CHAIXubin, ZHANGLimin, LIANGShuangshuang. Efficacy of electric conduction on rats with penicillin-induced neocortical epilepsy. Journal of Epilepsy, 2016, 2(1): 38-42. doi: 10.7507/2096-0247.20160008 Copy

1.	Banerjee PN, Filippi D, Allen Hauser W. The descriptive epidemiology of epilepsy: a review. Epilepsy Res, 2009, 85(12): 31-45.
2.	Seker Yilmaz B, Okuyaz C, Komur M. Predictors of intractable childhood epilepsy. Pediatr Neurol, 2013, 48(7): 52-55.
3.	Liang S, Li A, Zhao M, et al. Anterior temporal lobectomy combined with anterior corpus callosotomy in patients with temporal lobe epilepsy and mental retardation. Seizure, 2010, 19(9): 330-334.
4.	Jaseja H. Pedunculopontine nucleus stimulation in intractable epilepsy: Simulation of nature's antiepileptic role and mechanism. Epilepsy Behav, 2013, 27(12): 507.
5.	Liang S, Li A, Jiang H, et al. Anterior corpus callosotomy in patients with intractable generalized epilepsy and mental retardation. Stereotact Funct Neurosurg, 2010, 88(13): 246-252.
6.	Liang S, Liu T, Li A, et al. Long-term follow up of very low-dose LINAC based stereotactic radiotherapy in temporal lobe epilepsy. Epilepsy Res, 2010, 90(17): 60-67.
7.	梁爽爽, 梁树立, 于晓曼, 等.儿童部分性癫痫切除性手术长期疗效分析.中华神经外科杂志, 2013, 29(8): 12-16.
8.	梁树立, 李安民, 张继武, 等.深部电极脑电图在灰质异位相关癫痫中的应用.中华神经外科杂志, 2013, 27(11): 166-168.
9.	Goldberg-Stern H, Feldman L, Eidlitz-Markus T, et al. Levetiracetam in children, adolescents and young adults with intractable epilepsy: efficacy, tolerability and effect on electroencephalogram--a pilot study. Eur J Paediatr Neurol, 2013, 17(2): 248-253.
10.	Chen N, Yan N, Liu C, et al. Neuroprotective effects of electrical stimulation of the anterior nucleus of the thalamus for hippocampus neurons in intractable epilepsy. Med Hypotheses, 2013, 80(14): 517-519.
11.	梁树立.脑皮层痫性放电外引导电极.国家知识产权局. 2011, 专利号: ZL201020644725.1.
12.	White AM, Williams PA, Ferraro DJ, et al. Efficient unsupervised algorithms for the detection of seizures in continuous EEG recordings from rats after brain injury. J Neurosci Methods, 2006, 152(30): 255-66.
13.	Sitthinamsuwan B, Nunta-aree S. Surgical treatment of epilepsy: principles and presurgical evaluation. J Med Assoc Thai, 2013, 96(21): 121-131.
14.	梁树立, 姚世斌, 赵明, 等.颞叶癫痫的痫性放电传导和临床症状的颅内电极脑电图研究.中华神经外科杂志, 2009, 25(7): 816-818.
15.	Trevelyan AJ, Sussillo D, Yuste R. Feed forward inhibition contributes to the control of epileptiform propagation speed. J Neurosci, 2007, 27(11): 3383-3387.

1. Banerjee PN, Filippi D, Allen Hauser W. The descriptive epidemiology of epilepsy: a review. Epilepsy Res, 2009, 85(12): 31-45.
2. Seker Yilmaz B, Okuyaz C, Komur M. Predictors of intractable childhood epilepsy. Pediatr Neurol, 2013, 48(7): 52-55.
3. Liang S, Li A, Zhao M, et al. Anterior temporal lobectomy combined with anterior corpus callosotomy in patients with temporal lobe epilepsy and mental retardation. Seizure, 2010, 19(9): 330-334.
4. Jaseja H. Pedunculopontine nucleus stimulation in intractable epilepsy: Simulation of nature's antiepileptic role and mechanism. Epilepsy Behav, 2013, 27(12): 507.
5. Liang S, Li A, Jiang H, et al. Anterior corpus callosotomy in patients with intractable generalized epilepsy and mental retardation. Stereotact Funct Neurosurg, 2010, 88(13): 246-252.
6. Liang S, Liu T, Li A, et al. Long-term follow up of very low-dose LINAC based stereotactic radiotherapy in temporal lobe epilepsy. Epilepsy Res, 2010, 90(17): 60-67.
7. 梁爽爽, 梁树立, 于晓曼, 等.儿童部分性癫痫切除性手术长期疗效分析.中华神经外科杂志, 2013, 29(8): 12-16.
8. 梁树立, 李安民, 张继武, 等.深部电极脑电图在灰质异位相关癫痫中的应用.中华神经外科杂志, 2013, 27(11): 166-168.
9. Goldberg-Stern H, Feldman L, Eidlitz-Markus T, et al. Levetiracetam in children, adolescents and young adults with intractable epilepsy: efficacy, tolerability and effect on electroencephalogram--a pilot study. Eur J Paediatr Neurol, 2013, 17(2): 248-253.
10. Chen N, Yan N, Liu C, et al. Neuroprotective effects of electrical stimulation of the anterior nucleus of the thalamus for hippocampus neurons in intractable epilepsy. Med Hypotheses, 2013, 80(14): 517-519.
11. 梁树立.脑皮层痫性放电外引导电极.国家知识产权局. 2011, 专利号: ZL201020644725.1.
12. White AM, Williams PA, Ferraro DJ, et al. Efficient unsupervised algorithms for the detection of seizures in continuous EEG recordings from rats after brain injury. J Neurosci Methods, 2006, 152(30): 255-66.
13. Sitthinamsuwan B, Nunta-aree S. Surgical treatment of epilepsy: principles and presurgical evaluation. J Med Assoc Thai, 2013, 96(21): 121-131.
14. 梁树立, 姚世斌, 赵明, 等.颞叶癫痫的痫性放电传导和临床症状的颅内电极脑电图研究.中华神经外科杂志, 2009, 25(7): 816-818.
15. Trevelyan AJ, Sussillo D, Yuste R. Feed forward inhibition contributes to the control of epileptiform propagation speed. J Neurosci, 2007, 27(11): 3383-3387.

Previous Article
The dynamic expression of Robo3 in the hippocampus of the lithium-pilocarpine induced rat model
Next Article
癫痫持续状态研究进展

Journal of Epilepsy

Efficacy of electric conduction on rats with penicillin-induced neocortical epilepsy

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content