Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus_Journal of Epilepsy

Authors：

LV Minghui , SU Shaohua , WU Xin , ZHENG Wanjun , ZHANG Songjiang , SUN Ningning ,  GAO Jianfeng

School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;

Corresponding author：

GAO Jianfeng, Email: jc.gjfeng@hotmail.com

Keywords：

Electroacupuncture; Status epilepticus; Hippocampus; Neurons; Apoptosis; Mice

DOI：

10.7507/2096-0247.20180036

Video：

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ObjectiveTo investigate the effect of electroacupuncture on the apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus by observing the changes of hippocampal subtle neuron pathology and apoptosis.MethodsMale C57BL/6J mice were used to prepare epileptic status models of lithium-pilocarpine mice, and then 7-day electroacupuncture stimulation (Baihui, Fengfu) were given to the mice model. Open field experiment and new object recognition experiment were performed to observe the changes of cognitive abilities. The pathological changes of hippocampal neurons were detected by HE staining. Hippocampal apoptosis protein (Caspase-3) and microtubule-associated protein (MAP-2) were detected by immunohistochemistry. Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus were recorded.Results① Compared with the control group, the vertical movement, modification times, and number of crossings of the model group all decreased significantly (P<0.000 1,P<0.000 1,P<0.000 1), and their cognitive ability decreased significantly (P<0.01). Compared with the model group, vertical movements, modification times, and number of crossings were increased in the electroacupuncture (EA) group (P<0.01,P<0.05,P<0.05), and the cognitive ability of new objects was increased (P<0.01). ② HE staining showed that the model group had significant damage to the hippocampal neurons of mice, and the cells swelled, nuclear collapsed and vacuoles appeared. In the EA group, the injury of hippocampal neurons was alleviated, and cell edema and vacuolization were alleviated. ③ Immunohistochemistry showed that compared with the control group, the IOD of the Caspase-3 positive cells in the hippocampus of the model group increased significantly (P<0.000 1), and the IOD of the MAP-2 positive cells decreased significantly (P<0.01); Compared with the electroacupuncture, the IOD of the Caspase-3 positive cells in the hippocampus of the mice decreased (P<0.05), and the IOD of the MAP-2 positive cells increased (P<0.05).ConclusionsElectroacupuncture can improve the pathological changes of hippocampal neurons in C57BL/6J mice with status epilepticus, promote cytoskeletal repair, reduce neuronal apoptosis in hippocampus, and antagonize the damage of hippocampal neurons induced by status epilepticus.

Citation： LV Minghui, SU Shaohua, WU Xin, ZHENG Wanjun, ZHANG Songjiang, SUN Ningning, GAO Jianfeng. Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus. Journal of Epilepsy, 2018, 4(3): 207-213. doi: 10.7507/2096-0247.20180036 Copy

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19.	昂文平, 杨帆, 沈德凯, 等. 针刺对戊四唑致痫大鼠海马神经元超微结构的影响. 中国中医急症, 2012, 21(03): 380-382.
20.	Zeng XC, Chen SY, Gao Q, et al. The expression of G protein-coupled receptor kinase 5 and its interaction with dendritic marker microtubule-associated protein-2 after status epilepticus. Epilepsy Res, 2017, 138: 62-70.

1. 吕梦娜, 张洪. 难治性癫痫持续状态研究进展.中华实用诊断与治疗杂志, 2015, 3(29): 212-214.
2. Elger CE, Schmidt D. Modern management of epilepsy: a practical approach. Epilepsy Behav, 2008, 12(4): 501-539.
3. Kobow K, Auvin S, Jensen F, et al. Finding a better drug for epilepsy: antiepileptogenesis targets. Epilepsia, 2012,53(11): 1868-1876.
4. 张冬青. 抗癫痫药物对癫痫幼鼠认知的影响及其机制的研究. 山东大学, 2013: 21-26/56-60.
5. 高剑峰. 海马神经干细胞增生分化及影响因素. 郑州大学, 博士学位论文, 2010: 36-40.
6. Racine RJ. Modification of seizure activity by electrical Ⅱ motor seizure. Electroencephalogr Clin Neurophysiol, 1972, 32(3): 281-294.
7. 余曙光. 实验针灸学. 上海, 上海科学技术出版社, 2009: 149-150.
8. 陈少宗. 现代针灸学理论与临床应用. 济南, 黄河出版社, 1990: 36-37.
9. 武鑫, 郑婉君, 李琳, 等. 不同疗程电针对 X 线照射小鼠行为学影响实验研究. 中华放射肿瘤学杂志, 2016, 25(6): 634-637.
10. Fan XW, Chen F, Chen Y, et al. Electroacupuncture prevents cognitive impairments by regulating the early changes after brain irradiation in rats. PLoS One, 2015, 10(4): e0122087.
11. 胡明, 朱坤, 张建水, 等. 小鼠颞叶癫痫慢性期 CA3 区损伤对齿状回神经再生的影响. 西安交通大学学报(医学版), 2016, 4(37): 473-477.
12. 刘友平, 刘田, 毕航, 等. 品系、性别和生物节律对小鼠旷场实验的影响. 西安交通大学学报(医学版), 2014, 35(5): 634-638.
13. Alnemri ES, Livingston DJ, Nicholson DW, et al. Human ICE/CED-3 protease nomenclature. Cell, 1996, 87(2): 171.
14. Salvesen GS. Caspases:opening the boxes and interpreting the arrows. Cell Death Differ, 2002, 9(1): 3-5.
15. Ghavami S, Hashemi M, Ande SR, et al. Apoptosis and cancer: mutations within caspase genes. J Med Genet, 2009, 46(8): 497-510.
16. Cohen GM. Caspases: the executioners of apoptosis. Biochem J, 1997, 326(Pt1): 1-16.
17. Lim RW, Halpain S. Regulated association of microtubule-associated protein 2 (MAP2) with Src and Grb2: evidence for MAP2 as a scaffolding protein. J Biol Chem, 2000, 275(27): 20578-20587.
18. Rosser AE, Tyers P, ter Borg M, et al. Co-expression of MAP-2 and GFAP in cells developing from rat EGF responsive precursor cells. Brain Res Dev Brain Res, 1997, 98(2): 291-295.
19. 昂文平, 杨帆, 沈德凯, 等. 针刺对戊四唑致痫大鼠海马神经元超微结构的影响. 中国中医急症, 2012, 21(03): 380-382.
20. Zeng XC, Chen SY, Gao Q, et al. The expression of G protein-coupled receptor kinase 5 and its interaction with dendritic marker microtubule-associated protein-2 after status epilepticus. Epilepsy Res, 2017, 138: 62-70.

Journal of Epilepsy

Effect of electroacupuncture on apoptosis of hippocampal neurons in C57BL/6J mice with status epilepticus

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