Correlation study on human herpesvirus 6B and intractable mesial temporal lobe epilepsy_Journal of Epilepsy

Authors：

 WANG Huanming ¹ , HU Fei ¹ , LUO Minhua ² , ZHAO Fei ² , CHEN Jun ¹ , XIONG Yubo ¹

1. Department of Neurosurgery, Wuhan Brain Hospital & Changhang General Hospital, Wuhan 430010, China;
2. Wuhan Institute of Virology,Chinese Academy of Sciences.Wuhan 430070, China;

Corresponding author：

WANG Huanming, Email: 1808381741@qq.com

Keywords：

Human herpesvirus 6B; Medial temporal lobe epilepsy; Polymerase chain reaction

DOI：

10.7507/2096-0247.20200050

Video：

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Objective To explore the pathogenic mechanism of human herpesvirus 6B (HHV-6B) in the intractable mesial temporal lobe epilepsy (MTLE).Methods 42 cases of intractable MTLE and 17 cases of non-MTLE patients who treated in Wuhan Brain Hospital from Jan. 2012 to Dec. 2018 were enrolled in this study. Resected hippocampus, amygdala and mixed uncus samples were examined by real-time polymerase chain reaction (PCR) and reverse-transcriptase PCR to detect viral DNA and message RNA. Comparative analysis was used between the clinical characteristics and the HHV-6 detection.Results Detection of HHV-6 DNA was higher in MTLE patients (40.48%) than non-MTLE patients (11.7%). HHV-6B viral DNA was determined in 15/30 HHV-6 DNA-positive samples, and no HHV-6B mRNA were detected in all HHV-6 DNA negative samples. The patient’s age was the only influencing factor of HHV-6 expressing in MTLE. While the patient’s gender, onset age and possible disease cause had no effect on the HHV-6 content.Conclusions This study suggests that HHV-6B may play an important role in the pathogenesis of MTLE, but the mechanism needs further study.

Citation： WANG Huanming, HU Fei, LUO Minhua, ZHAO Fei, CHEN Jun, XIONG Yubo. Correlation study on human herpesvirus 6B and intractable mesial temporal lobe epilepsy. Journal of Epilepsy, 2020, 6(4): 315-319. doi: 10.7507/2096-0247.20200050 Copy

1.	Kawamura Y, Nakayama A, Kato T, et al. Pathogenic role of human herpesvirus 6B infection in mesial temporal lobe epilepsy. J Infect Dis, 2015, 212(7): 1014-1021.
2.	Huang C, Yan B, Lei D, et al. Apolipoprotein 4 may increase viral load and seizure frequency in mesial temporal lobe epilepsy patients with positive human herpes virus 6B. Neurosci Lett, 2015, 593: 29-34.
3.	Esposito L, Drexler JF, Braganza O, et al. Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies. Epilepsia, 2015, 56(2): 234-243.
4.	Sellner J, Trinka E. Seizures and epilepsy in herpes simplex virus encephalitis: current concepts and future directions of pathogenesis and management. J Neurol, 2012, 259(10): 2019-2030.
5.	Harberts E, Yao K, Wohler JE, et al. Human herpesvirus-6 entry into the central nervous system through the olfactory pathway. Proc Natl Acad Sci USA, 2011, 108(33): 13734-13739.
6.	Li JM, Lei D, Peng F, et al. Detection of human herpes virus 6B in patients with mesial temporal lobe epilepsy in West China and the possible association with elevated NF-κB expression. Epilepsy Res, 2011, 94(1-2): 1-9.
7.	Niehusmann P, Mittelstaedt T, Bien CG. Presence of human herpes virus 6 DNA exclusively in temporal lobe epilepsy brain tissue of patients with history of encephalitis. Epilepsia, 2010, 51(12): 2478-2483.
8.	Theodore WH, Epstein L, Gaillard WD, et al. Human herpes virus 6B: a possible role in epilepsy? Epilepsia, 2008, 49(11): 1828-1837.
9.	Karatas H, Gurer G, Pinar A, et al. Investigation of HSV-1, HSV-2, CMV, HHV-6 and HHV-8 DNA by real-time PCR in surgical resection materials of epilepsy patients with mesial temporal lobe sclerosis. J Neurol Sci, 2008, 264(1-2): 151-156.
10.	Yao K, Crawford JR, Komaroff AL, et al. Review part 2: Human herpesvirus-6 in central nervous system diseases. J Med Virol, 2010, 82(10): 1669-1678.
11.	Fotheringham J, Donati D, Akhyani N, et al. Association of human herpesvirus-6B with mesial temporal lobe epilepsy. PLoS Med, 2007, 4(5): e180.
12.	Yamashita N, Morishima T. HHV-6 and seizures. Herpes, 2005, 12(2): 46-49.
13.	Eeg-Olofsson O, Bergström T, Andermann F, et al. Herpesviral DNA in brain tissue from patients with temporal lobe epilepsy. Acta Neurol Scand, 2004, 109(3): 169-174.
14.	Millichap JJ, Millichap JG. Role of HHV-6B infection in mesial temporal lobe epilepsy. Pediatr Neurol Briefs, 2015, 29(5): 40.
15.	Donati D, Akhyani N, Fogdell-Hahn A, et al. Detection of human herpesvirus-6 in mesial temporal lobe epilepsy surgical brain resections. Neurology, 2003, 61(10): 1405-1411.
16.	Uesugi H, Shimizu H, Maehara T, et al. Presence of human herpesvirus 6 and herpes simplex virus detected by polymerase chain reaction in surgical tissue from temporal lobe epileptic patients. Psychiatry Clin Neurosci, 2000, 54(5): 589-593.
17.	Epstein LG, Shinnar S, Hesdorffer DC, et al. Human herpesvirus 6 and 7 in febrile status epilepticus: the FEBSTAT study. Epilepsia, 2012, 53(9): 1481148-8.
18.	Seeley WW, Marty FM, Holmes TM, et al. Post-transplant acute limbic encephalitis: clinical features and relationship to HHV6. Neurology, 2007, 69(2): 156-165.
19.	Howell KB, Tiedemann K, Haeusler G, et al. Symptomatic generalized epilepsy after HHV6 posttransplant acute limbic encephalitis in children. Epilepsia, 2012, 53(7): e122-e126.

1. Kawamura Y, Nakayama A, Kato T, et al. Pathogenic role of human herpesvirus 6B infection in mesial temporal lobe epilepsy. J Infect Dis, 2015, 212(7): 1014-1021.
2. Huang C, Yan B, Lei D, et al. Apolipoprotein 4 may increase viral load and seizure frequency in mesial temporal lobe epilepsy patients with positive human herpes virus 6B. Neurosci Lett, 2015, 593: 29-34.
3. Esposito L, Drexler JF, Braganza O, et al. Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies. Epilepsia, 2015, 56(2): 234-243.
4. Sellner J, Trinka E. Seizures and epilepsy in herpes simplex virus encephalitis: current concepts and future directions of pathogenesis and management. J Neurol, 2012, 259(10): 2019-2030.
5. Harberts E, Yao K, Wohler JE, et al. Human herpesvirus-6 entry into the central nervous system through the olfactory pathway. Proc Natl Acad Sci USA, 2011, 108(33): 13734-13739.
6. Li JM, Lei D, Peng F, et al. Detection of human herpes virus 6B in patients with mesial temporal lobe epilepsy in West China and the possible association with elevated NF-κB expression. Epilepsy Res, 2011, 94(1-2): 1-9.
7. Niehusmann P, Mittelstaedt T, Bien CG. Presence of human herpes virus 6 DNA exclusively in temporal lobe epilepsy brain tissue of patients with history of encephalitis. Epilepsia, 2010, 51(12): 2478-2483.
8. Theodore WH, Epstein L, Gaillard WD, et al. Human herpes virus 6B: a possible role in epilepsy? Epilepsia, 2008, 49(11): 1828-1837.
9. Karatas H, Gurer G, Pinar A, et al. Investigation of HSV-1, HSV-2, CMV, HHV-6 and HHV-8 DNA by real-time PCR in surgical resection materials of epilepsy patients with mesial temporal lobe sclerosis. J Neurol Sci, 2008, 264(1-2): 151-156.
10. Yao K, Crawford JR, Komaroff AL, et al. Review part 2: Human herpesvirus-6 in central nervous system diseases. J Med Virol, 2010, 82(10): 1669-1678.
11. Fotheringham J, Donati D, Akhyani N, et al. Association of human herpesvirus-6B with mesial temporal lobe epilepsy. PLoS Med, 2007, 4(5): e180.
12. Yamashita N, Morishima T. HHV-6 and seizures. Herpes, 2005, 12(2): 46-49.
13. Eeg-Olofsson O, Bergström T, Andermann F, et al. Herpesviral DNA in brain tissue from patients with temporal lobe epilepsy. Acta Neurol Scand, 2004, 109(3): 169-174.
14. Millichap JJ, Millichap JG. Role of HHV-6B infection in mesial temporal lobe epilepsy. Pediatr Neurol Briefs, 2015, 29(5): 40.
15. Donati D, Akhyani N, Fogdell-Hahn A, et al. Detection of human herpesvirus-6 in mesial temporal lobe epilepsy surgical brain resections. Neurology, 2003, 61(10): 1405-1411.
16. Uesugi H, Shimizu H, Maehara T, et al. Presence of human herpesvirus 6 and herpes simplex virus detected by polymerase chain reaction in surgical tissue from temporal lobe epileptic patients. Psychiatry Clin Neurosci, 2000, 54(5): 589-593.
17. Epstein LG, Shinnar S, Hesdorffer DC, et al. Human herpesvirus 6 and 7 in febrile status epilepticus: the FEBSTAT study. Epilepsia, 2012, 53(9): 1481148-8.
18. Seeley WW, Marty FM, Holmes TM, et al. Post-transplant acute limbic encephalitis: clinical features and relationship to HHV6. Neurology, 2007, 69(2): 156-165.
19. Howell KB, Tiedemann K, Haeusler G, et al. Symptomatic generalized epilepsy after HHV6 posttransplant acute limbic encephalitis in children. Epilepsia, 2012, 53(7): e122-e126.

Journal of Epilepsy

Correlation study on human herpesvirus 6B and intractable mesial temporal lobe epilepsy

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