The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination_West China Medical Journal

Authors：

JIANG Shijie ¹ , YANG Xiaofang ¹ , LI Jingmei ²

1.Department of Neurology，Sui ning People’s Hospital，Suining Sichuan，629000;2.Department of Neurology，West China Hospitall，Sichuan University，Chengdu 640041，China;

Corresponding author：

Keywords：

难治性癫痫; 蛋白表达; 免疫荧光

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目的:β淀粉样蛋白（β-amyloid precursor protein，β-APP）是已知的参与阿尔茨海默病机制的关键因子。β-APP是否参与难治性癫痫中的病理机制并不清楚。这项研究在于了解β-APP的蛋白在难治性癫痫患者术后颞叶皮质和海马组织中的表达是否异常。方法:免疫荧光法半定量测定难治性癫痫患者术后颞叶皮质和海马组织中的β-APP阳性蛋白的荧光值，并应用统计软件对实验数据进行单因素方差分析。结果:免疫荧光强度值分析结果显示β-APP在耐药性癫痫脑组织中表达较对照组明显增高且有统计学意义。结论:β-APP在难治性癫痫脑组织中异常增高，增高的β-APP可能参与了难治性癫痫的病理机制。

Citation： JIANG Shijie,YANG Xiaofang,LI Jingmei. The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination. West China Medical Journal, 2009, 24(1): 32-34. doi: Copy

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1. Salinero O，MorenoFlores MT，Wandosell F.Increasing neurite outgrowth capacity of βamyloid precursor protein proteoglycan in Alzheimer’s disease[J].J Neurosci Res，2000，60（1）:87-97..
2. 吴逊，沈鼎烈.难治性癫痫[J].中华神经科杂志，1998，31:4..
3. 王学峰，肖波，孙红斌.难治性癫痫[M].上海科学技术出版社，2001:5-11..
4. Perez RG，Zheng H，Van der Ploeg LH，et al.The βamyloid precursor protein of Alzheimer’s disease enhances neuron viability and modulates neuronal polarity[J].J Neurosci，1997，17（24）:9407-9414..
5. Niess C，Grauel U，Toennes SW，et al.Incidence of axonal injury in human brain tissue[J].Acta Neuropathol（Berl），2002，104（1）:79-84..
6. Smith DH，Chen XH，Iwata A，et al.Amyloid β accumulation in axons after traumatic brain injury in humans[J].J Neurosurg，2003，98（5）:1072-1077..
7. GordonKrajcer W，Gajkowska B.Excitotoxicityinduced expression of amyloid precursor protein （βAPP） in the hippocampus and cortex of rat brain.An electronmicroscopy and biochemical study[J].Folia Neuropathol，2001，39（3）:163-173..
8. 汪建华，王学峰，龚云，等.耐药性癫痫患者术后脑组织中Tau蛋白的表达[J].中华神经科杂志，2005，38（9）:543-546..
9. Tomizawa K，Cai XH，Moriwaki A，et al.Involvement of cyclindependent kinase 5/p35（nck5a） in the synaptic reorganization of rat hippocampus during kindling progression[J].Jpn J Physiol，2000，50（5）:525-532.
10. Pollard H，Khrestchatisky M，Moreau J，et al.Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus[J].Nuroscience，1994，63（2）:627..
11. Hoshi M，Sato M，Matsumoto S，et al.Spherical aggregates of βamyloid （amylospheroid） show high neurotoxicity and activate tau protein kinase Ⅰ/glycogen synthase kinase3β[J].Proc Natl Acad Sci USA，2003，100（11）:6370-6375..
12. Han P，Dou F，Li F，et al.Suppression of cyclindependent kinase 5 activation by amyloid precursor protein:a novel excitoprotective mechanism involving modulation of tau phosphorylation[J].J Neurosci，2005，25（50）:11542-11552..
13. Takashima A，Honda T，Yasutake K，et al.Activation of tau protein kinase Ⅰ/glycogen synthase kinase3β by amyloid β peptide （2535） enhances phosphorylation of tau in hippocampal neurons[J].Neurosci Res，1998，31（4）:317-323..
14. Lee MS，Kwon YT，Li M，et al.Neurotoxicity induces cleavage of p35 to p25 by calpain[J].Nature，2000，405（6784）:360-364..
15. Greenberg SM，Koo EH，Selkoe DJ，et al.Secreted βamyloid precursor protein stimulates mitogenactivated protein kinase and enhances tau phosphorylation[J].Proc Natl Acad Sci USA，1994，91（15）:7104-7108.

West China Medical Journal

The Expression of β-amyloid Precursor Protein in Brain Tissue of Pharmacoresistant Epilepsy Patients by Immunofluorescence Examination

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