Effect of TRAIL to Reverse the DDP resistance of Ovarian Carcinoma Cell Line COC1/DDP_West China Medical Journal

Authors：

LI Chunmei , PENG Zhilan , YIN Rutie

Department of Gynecology and Obstetrics，West China Second Hospital，Sichuan University，Chengdu 610041，China;

Corresponding author：

Keywords：

卵巢肿瘤; TRAIL; 顺铂; 药物耐受性

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目的:研究TRAIL对卵巢癌COC1/DDP细胞生长的影响，以及化疗药物DDP等对TRAIL受体（DR4、DR5）表达的影响，揭示TRAIL与COC1/DDP细胞顺铂耐药性的关系。方法:用MTT法检测不同浓度TRAIL蛋白和TRAIL与DDP联合用药对COC1/DDP细胞生长的影响，用RTPCR方法检测DDP对TRAIL受体（DR4、DR5）表达的影响。结果:①TRAIL蛋白对COC1/DDP细胞生长有抑制作用，且随着TRAIL蛋白浓度升高，细胞抑制率逐渐上升。②DDP（2.5μg/mL）对COC1/DDP细胞生长抑制作用较弱（抑制率为3.31%），DDP在加入TRAIL蛋白后对细胞生长抑制率显著升高（P lt;0.05）。③DDP使COC1/DDP细胞的DR5表达水平显著增强为正常对照组的3.54倍（P lt;0.001）。结论:TRAIL蛋白对COC1/DDP细胞生长有抑制作用，DDP与TRAIL联合使用COC1/DDP细胞生长抑制更明显，TRAIL可逆转COC1/DDP细胞对DDP的耐药性，耐药性的逆转可能与DDP导致TRAIL受体DR5水平增高促进了肿瘤细胞的凋亡有关。

Citation： LI Chunmei,PENG Zhilan,YIN Rutie. Effect of TRAIL to Reverse the DDP resistance of Ovarian Carcinoma Cell Line COC1/DDP. West China Medical Journal, 2009, 24(1): 105-107. doi: Copy

1.	Griffith TS，Chin WA，Jackson GC，et al. Intracellular regulation of TRAILinduced apoptosis in human melanoma cells[J].J Immunol，1998，161（6）:2833-2840..
2.	Wiley SR，Schooley K，Smolak PJ，et al.Indentification and characterization of a new member of the TNF family that induces apoptosis[J].Immunity，1995，3（6）:673-682..
3.	Sheridan JP，Marsters SA，Pitti RM，et al.Control of TRAILinduced apoptosis by a family of signaling and decoy receptors[J].Science，1997，277（5327）:818-821..
4.	Sprick MR，Weigand MA，Rieser E，et al.FADD/MORT1 and caspase8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2[J].Immunity，2000，12（6）:599-609..
5.	Thomas LR，Henson A，Reed JC，et al.Direct binding of Fasassociated death domain （FADD） to the tumor necrosis factorrelated apoptosisinducing ligand receptor DR5 is regulated by the death effector domain of FADD[J].J Biol Chem，2004，279（31）:32780-32785..
6.	李春梅，彭芝兰，尹如铁.TRAIL蛋白对卵巢癌COC1和COC1/DDP细胞生长及促凋亡作用研究[J].四川肿瘤防治，2006，19（3）:161-166..
7.	Cuello M，Ettenberg SA，Nau MM，et al.Synergistic induction of apoptosis by the combination of TRAIL and chemotherapy in chemoresistant ovarian cancer cells[J].Gynecol Oncol，2001，81（3）:380-390..
8.	Arts HJ，de Jong S，Hollema H，et al.Chemotherapy induces death receptor 5 in epithelial ovarian carcinoma[J].Gynecol Oncol，2004，92（3）:794-800..
9.	Tomek S，Horak P，Pribill I，et al.Resistance to TRAILinduced apoptosis in ovarian cancer cell lines is overcome by cotreatment with cytotoxic drugs[J].Gynecol Oncol，2004，94（1）:107-114..

1. Griffith TS，Chin WA，Jackson GC，et al. Intracellular regulation of TRAILinduced apoptosis in human melanoma cells[J].J Immunol，1998，161（6）:2833-2840..
2. Wiley SR，Schooley K，Smolak PJ，et al.Indentification and characterization of a new member of the TNF family that induces apoptosis[J].Immunity，1995，3（6）:673-682..
3. Sheridan JP，Marsters SA，Pitti RM，et al.Control of TRAILinduced apoptosis by a family of signaling and decoy receptors[J].Science，1997，277（5327）:818-821..
4. Sprick MR，Weigand MA，Rieser E，et al.FADD/MORT1 and caspase8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2[J].Immunity，2000，12（6）:599-609..
5. Thomas LR，Henson A，Reed JC，et al.Direct binding of Fasassociated death domain （FADD） to the tumor necrosis factorrelated apoptosisinducing ligand receptor DR5 is regulated by the death effector domain of FADD[J].J Biol Chem，2004，279（31）:32780-32785..
6. 李春梅，彭芝兰，尹如铁.TRAIL蛋白对卵巢癌COC1和COC1/DDP细胞生长及促凋亡作用研究[J].四川肿瘤防治，2006，19（3）:161-166..
7. Cuello M，Ettenberg SA，Nau MM，et al.Synergistic induction of apoptosis by the combination of TRAIL and chemotherapy in chemoresistant ovarian cancer cells[J].Gynecol Oncol，2001，81（3）:380-390..
8. Arts HJ，de Jong S，Hollema H，et al.Chemotherapy induces death receptor 5 in epithelial ovarian carcinoma[J].Gynecol Oncol，2004，92（3）:794-800..
9. Tomek S，Horak P，Pribill I，et al.Resistance to TRAILinduced apoptosis in ovarian cancer cell lines is overcome by cotreatment with cytotoxic drugs[J].Gynecol Oncol，2004，94（1）:107-114..

West China Medical Journal

Effect of TRAIL to Reverse the DDP resistance of Ovarian Carcinoma Cell Line COC1/DDP

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