目的:研究小鼠头颈鳞癌细胞株SCCⅦ体外放射敏感性,并探讨其与细胞周期阻滞的可能关系。方法:利用细胞克隆形成试验及MTT法检测SCCⅦ细胞受X射线照射后细胞存活能力及细胞生长趋势的变化,通过流式细胞学检测X射线照射后细胞周期分布的变化。结果:相同剂量照射后的SCCⅦ细胞存活分数高于Hela细胞(P lt;0.05);4 Gy照射后的SCCⅦ细胞在96 h内细胞生长速度仍高于Hela细胞(P lt;0.05);4 Gy照射后SCCⅦ细胞G1期和G2期细胞比例明显升高(P lt;0.05)。结论:SCCⅦ细胞对放射线不敏感性,放射线导致的细胞周期阻滞是SCCⅦ细胞放射抵抗的可能原因之一
Citation:
ZOU Jian,YE Huiping,YANG Yuqiong,et al. In Vitro Radiosensitivity Study of Head and Neck Squamous Carcinoma Cell SCCⅦ. West China Medical Journal, 2009, 24(1): 127-129. doi:
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Sakata K,Someya M,Matsumoto Y,et al.Ability to repair DNA doublestrand breaks related to cancer susceptibility and radiosensitivity[J].Radiat Med,2007,25(9):433-438.[9]Wilding CS,Curwen GB,Tawn EJ,et al.Influence of polymorphisms at loci encoding DNA repair proteins on cancer susceptibility and G2 chromosomal radiosensitivity[J].Environ Mol Mutagen,2007,48(1):48-57.
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- 1. Sakata K,Someya M,Matsumoto Y,et al.Ability to repair DNA doublestrand breaks related to cancer susceptibility and radiosensitivity[J].Radiat Med,2007,25(9):433-438.[9]Wilding CS,Curwen GB,Tawn EJ,et al.Influence of polymorphisms at loci encoding DNA repair proteins on cancer susceptibility and G2 chromosomal radiosensitivity[J].Environ Mol Mutagen,2007,48(1):48-57.