Proliferation and Invasion Inhibition of Na+,K+-ATP1b1 on Hepatocellular Carcinoma Cell_West China Medical Journal

Authors：

1Department of General Surgery,West China Hospital,Sichuan University,Chengdu610041,China; 2Division of Experimental Oncology,State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China;

Corresponding author：

Keywords：

Na+，K+-ATP1b1; 肝癌细胞; 生长; 侵袭

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目的：探讨Na+，K+-ATP1b1（ATP1b1）对肝癌细胞生长及侵袭能力的影响。方法：将ATP1b1表达质粒ATP1b1-CMV-FLAG转染肝癌细胞SMMC-7721，以空质粒转染作对照，通过RT-PCR测定ATP1b1的mRNA表达，在光镜下观察细胞形态学变化，MTT法检测细胞生长增殖，Transwell细胞侵袭实验分析肝癌细胞的侵袭能力。结果：ATP1b1-CMV-FLAG转染后，SMMC-7721的ATP1b1 mRNA表达水平明显增高（P＜0.05）,转染ATP1b1-CMV-FLAG组、pFLAG-CMV-1组和脂质体组的ATP1b1 mRNA表达水平分别为1.159、0.182和0.093;转染ATP1b1-CMV-FLAG的SMMC-7721细胞呈变性改变，其生长受到抑制，转染相同DNA浓度的ATP1b1-CMV-FLAG组与pFLAG-CMV-1组对细胞生长的抑制效应有显著性差异（P＜0.05）；Transwell细胞侵袭实验显示转染ATP1b1-CMV-FLAG组的SMMC-7721细胞的体外侵袭力明显受抑制（P＜0.01）。结论：实验结果说明了促进ATP1b1表达能抑制肝癌细胞的生长及侵袭行为。

Citation： LIU Zhanpei,XIONG Zhujuan,ZHANG Jie,et al.. Proliferation and Invasion Inhibition of Na+,K+-ATP1b1 on Hepatocellular Carcinoma Cell. West China Medical Journal, 2008, 23(1): 94-96. doi: Copy

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1. secondary structure[Ｊ].J Mol Biol,1999,288:911-940.
2. chen Q,Watson JT,Marengo SR,et al.Gene expression in the LNCaP humanprostate cancer progression model: progression associated expression in vitrocorresponds to expression changes associated with prostate cancer progressionin vivo[Ｊ].Cancer Lett,2006,244(2):274-２88..
3. Le Page C,Ouellet V,Madore J,et al.Gene expression profiling of primary cultures of ovarian epithelial cells identifies novel molecular classifiers of ovarian cancer[Ｊ].Br J Cancer,2006,94(3):436-４45..
4. Lingrel JB,Kuntzweiler T.Na+,K(+)-ATPase.J Biol Chem,1994,269(31):19 659-19 662..
5. Shoshani L,Contreras RG,Roldan ML,et al.The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells[Ｊ].Mol Biol Cell,2005,16(3):1 071-81..
6. Mathews DH,Sabina J,Zuker M,et al.Expanded sequence dependence of thermodynamic parameters improves prediction of RNA.
7. Chow DC,Forte JG.Functional significance of the β-subunit for heterodimeric P-type ATPases[Ｊ].J Exp Biol,1995,198:1-17..
8. Fambrough DM,Lemas MV,Hamrick M,et al.Analysis of subunit assembly of the Na-K-ATPase[Ｊ].Am J Physiol,1994,266(3 Pt 1):C579-C589．.
9. Beguin P,Hasler U,Staub O,et al.Endoplasmic reticulum quality control of oligomeric membrane proteins:topogenic determinants involved in the degradation of the unassembled Na,K-ATPase alpha subunit and in its stabilization by beta subunit assembly[Ｊ].Mol Biol Cell,2000,11(5):1657-1672..
10. Pestov NB,Korneenko TV,Radkov R,et al.Identification of the β-subunit for nongastric H-K-ATPase in rat anterior prostate[Ｊ].Am J Physiol Cell Physiol,2004,286(6):C1229-C1237..
11. Rajasekaran SA,Gopal J,Willis D,et al.Na,K-ATPase beta1-subunit increases the translation efficiency of the alpha1-subunit in MSV-MDCK cells[Ｊ].Mol Biol Cell,2004,15(7):3224-3232.

West China Medical Journal

Proliferation and Invasion Inhibition of Na+,K+-ATP1b1 on Hepatocellular Carcinoma Cell

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