Expressions of Zinc Finger Protein A20，NF-κB p65 Protein，and P-glycoprotein in Primary Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

IU Jiao , YANG Jie , XIANG Ying ,  XU Mingqing

Department of Hepatovascular Surgery， West China Hospital， Sichuan University， Chengdu 610041， Sichuan Province， China;

Corresponding author：

XU Mingqing, Email: xumingqing0018@163.com

Keywords：

Primary hepatocellular carcinoma; Zinc finger protein A20; NF-κB p65 protein; P-glycoprotein; Immunohistochemistry

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Objective 　To compare the positive rate of zinc finger protein A20， NF-κB p65 protein， and P-glyco- protein between primary hepatocellular carcinoma （HCC） tissues and paratumor tissues， and to explore the relationship between the 3 kinds of proteins and pathological features of HCC.
Methods 　Thirty-two HCC tissues and 26 paratumor tissues resected from patients with HCC treated in our hospital from Feb. 2009 to Aug. 2010 were enrolled. Clinical data were also collected from files. The expressions of zinc finger protein A20， NF-κB p65 protein， and P-glycoprotein were tested by immunohistochemistry.
Results 　The positive rate of zinc finger protein A20， NF-κB p65 protein， and P-glycoprotein in HCC tissues were 87.5%（28/32）， 81.3%（26/32）， and 65.6%（21/32）， respectively， which were higher than that in paratumor tissues〔61.5%（16/26）， 34.6%（9/26）， and 30.8%（8/26）， respectively〕， P＜0.05. The three kinds of proteins were all closely related with HbsAg， and zinc finger protein A20 was related with cirrhosis in addition （P＜0.05）.
Conclusions 　The positive rate of zinc protein A20， NF-κB p65 protein， and P-glycoprotein are much higher in primary HCC tissues than that in paratumor tissus， and they may play an important role in preoperative determination of hepatic tumors.

Citation： IU Jiao,YANG Jie,XIANG Ying,XU Mingqing. Expressions of Zinc Finger Protein A20，NF-κB p65 Protein，and P-glycoprotein in Primary Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(12): 1302-1306. doi: Copy

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20.	Lacueva FJ， Calpena R， Medrano J， et al. Changes in P-glycoprotein expression in gastric carcinoma with respect to distantgastric mucosa may be influenced by p53[J]. Cancer， 2000， 89(1)： 21-28.

1. Nakajima T， Deguchi T， Kagawa K， et al. Identification ofapoptotic hepatocytes in situ in rat liver after lead nitrate administration[J]. J Gastroenterol， 1995， 30(6)： 725-730.
2. Kubo K， Matsuzaki Y， Okazaki M. The Fas system is notsignificantly involved in apoptosis in human hepatocellular carcinoma[J]. Liver， 1998， 18(2)： 117-123.
3. Heyninck K， De Valck D， Vanden Berghe W， et al. The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN[J] ． J Cell Biol， 1999， 145(7)： 1471-1482．.
4. Arsura M， Cavin LG. Nuclear factor-kappaB and liver carcinogenesis[J]. Cancer Lett， 2005， 229(2)： 157-169.
5. Hinz M， Krappmann D， Eichten A， et al. NF-kappaB function in growth control： regulation of cyclin D1 expression and G0/G1-to-S-phase transition[J]. Mol Cell Biol， 1999， 19(4)： 2690-2698.
6. 刘晓. 核转录因子NF-κB与肿瘤[J]. 实用肿瘤学杂志， 2007， 21(2)： 191-193.
7. Sharom FJ. Shedding light on drug transport： structure and function of the P-glycoprotein multidrug transporter (ABCB1)[J]. Biochem Cell Biol， 2006， 84(6)： 979-992.
8. Zhang F， Ren G， Lu Y， et al. Identification of TRAK1 (trafficking protein， kinesin-binding 1) as MGb2-Ag： a novel cancer biomarker[J]. Cancer Lett， 2009， 274(2)： 250-258.
9. Dixit VM， Green S， Sarma V， et al. Tumor necrosis factor-alpha induction of novel gene products in human endothelial cells including a macrophage-specific chemotaxin[J]. J Biol Chem， 1990， 265(5)： 2973-2978.
10. 徐明清，严律南，苟兴华，等. 锌指蛋白A20促进同种异体大鼠小体积移植肝脏再生并抑制急性排斥[J]. 中国普外基础与临床杂志， 2008， 15(8)： 572-578.
11. Amiri KI， Richmond A. Role of nuclear factor kappa B in melanoma[J]. Cancer Metastasis Rav， 2005， 24(2)： 301-313.
12. Pikarsky E， Porat RM， Stein I， et al. NF-kappaB function as a tumor promoter in inflammation-associated cancer[J]. Nature， 2004， 431(7007)： 461-466.
13. Sclabas GM， Uwagawa T， Schmidt C， et al. Nuclear factor kappa B activation is a potential target for preventing pancreatic carcinoma by aspirin[J]. Cancer， 2005， 103(12)： 2485-2490.
14. Kojima M， Morisaki T， Sasaki N， et al. Increased nuclear factor-κBactivation in human colorectal carcinoma and its correlation with tumor progression[J]. Anticancer Res， 2004， 24(2B)： 675-681.
15. Hanahan D， Weinberg RA. The hallmarks of cancer[J]. Cell， 2000， 100(1)： 57-70.
16. Aggarwal BB. Nuclear factor kappa B： the enemy within[J]. Cancer Cell， 2004，6(3)： 203-208.
17. 秦安，蔡兵，王彤，等. 肝细胞肝癌中survivin、caspase-3和NF-κB的表达及其相关性[J]. 南京医科大学学报：自然科学版， 2009， 29(7)： 953-957．.
18. Ruefli AA， Smyth MJ， Johnstone RW. HMBA induces activation of a caspase-independent cell death pathway to overcome P-glycoprotein-mediated multidrug resistance[J]. Blood， 2000， 95(7)： 2378-2385.
19. Campone M， Vavasseur F， Le Cabellec MT， et al. Induction of chemoresistance in HL-60 cells concomitantly causes a resistance to apoptosis and the synthesis of P-glycoprotein[J]. Leukemia， 2001， 15(9)： 1377-1387.
20. Lacueva FJ， Calpena R， Medrano J， et al. Changes in P-glycoprotein expression in gastric carcinoma with respect to distantgastric mucosa may be influenced by p53[J]. Cancer， 2000， 89(1)： 21-28.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions of Zinc Finger Protein A20，NF-κB p65 Protein，and P-glycoprotein in Primary Hepatocellular Carcinoma

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