Joint Detection of hTERT mRNA and GGT mRNA-H Expressions in Peripheral Blood of Small Hepatocellular Carcinoma Patients and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 SUI Lulu ¹ , LIU Xiaofang ² , XU Zheng ²

1.Medical College of Qingdao Universtiy, Qingdao 266071, Shandong Province, China;;
2.Department of Hepatobiliary and Pancreatic Surgery, The Affiliated Yuhuangding Hospital of Yantai, Medical College of Qingdao University, Yantai 264000, Shandong Province, China;

Corresponding author：

SUI Lulu , Email: suilulu07@163.com

Keywords：

Hepatocellular carcinoma; Telomerase; Human telomerase reverse transcriptase; γ-glutamyl transpeptidase; Polymerase chain reaction; Tumor

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ObjectiveTo investigate the expressions and clinical significance of human telomerase reverse transcriptase (hTERT) mRNA and γglutamyl transpeptidase mRNA-H (GGT mRNA-H) in the peripheral blood of small hepatocellular carcinoma (HCC) patients. MethodsThe expressions of hTERT mRNA and GGT mRNA-H were detected in the peripheral blood of thirty patients with small HCC by RT-PCR, eighteen patients with benign liver diseases, and twelve normal volunteers. ResultsThe positive rate of hTERT mRNA and GGT mRNA-H expression in patients with small HCC were 80.0% (24/30) and 46.7%(14/30), respectively. In patients with hepatitic cirrhosis the positive rate of hTERT mRNA expression was 33.3% (6/18), while the expression of GGT mRNA was not detected. Both the expressions of hTERT mRNA and GGT mRNA-H were negative in all normal volunteers. The combination analysis of hTERT mRNA and GGT mRNA-H expression achieved positive rate of 86.7% in the diagnosis of small HCC, which was significantly higher than the positive rate of AFP (26.7%)， P lt;0.05. ConclusionThe hTERT mRNA and GGT mRNA-H are significantly expressed in small HCC patients, the combination analysis of hTERT mRNA and GGT mRNA-H seems to be useful in the early diagnosis of small HCC.

Citation： SUI Lulu ,LIU Xiaofang,XU Zheng. Joint Detection of hTERT mRNA and GGT mRNA-H Expressions in Peripheral Blood of Small Hepatocellular Carcinoma Patients and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(1): 59-62. doi: Copy

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2.	Zhang Y, Li Q, Liu N, et al. Detection of MAGE1, MAGE3 and AFP mRNA as multimarker by realtime quantitative PCR assay: a possible predictor of hematogenous micrometastasis of hepatocellular carcinoma [J]. Hepatogastroenterology, 2008, 55(88): 22002206.
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4.	陈孝平, 张万广. 原发性肝癌面临的难点与对策 [J]. 中国普外基础与临床杂志， 2008, 15(4): 231233.
5.	沈啸洪, 李慧锴, 张倜. 肝癌外科规范化治疗若干问题探讨 [J]. 中国普外基础与临床杂志, 2009, 16(4): 261264.
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8.	中华医学会传染病与寄生虫病学分会、肝病学分会. 病毒性肝炎防治方案 [J]. 中华肝脏病杂志, 2000, 8(6): 324329.
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11.	Terrin L, Rampazzo E, Pucciarelli S. et al. Relationship between tumor and plasma levels of hTERT mRNA in patients with colorectal cancer: implications for monitoring of neoplastic disease [J]. Clin Cancer Res, 2008, 14(22): 74447451.
12.	Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
13.	Udomchaiprasertkul W, Narong S, Kongsema M, et al. Detection of hTERT mRNA in gastrointestinal tract cancer specimens [J]. Southeast Asian J Trop Med Public Health, 2008, 39(2): 324327.
14.	Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
15.	Capezzone M, Marchisotta S, Cantara S, et al. Telomeres and thyroid cancer [J]. Curr Genomics, 2009, 10(8): 526533.
16.	Perona R, MachadoPinilla R, Manguan C, et al. Telomerase deficiency and cancer susceptibility syndromes [J]. Clin Transl Oncol, 2009, 11(11): 711714.
17.	Stewart SA, Bertuch AA. The role of telomeres and telomerase in cancer research [J]. Cancer Res, 2010, 70(19): 73657371.
18.	Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells [J]. FEBS Lett, 2010, 584(17): 38193825.
19.	Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
20.	Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
21.	Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
22.	Theimer CA, Feigon J. Structure and function of telomerase RNA [J]. Curr Opin Struct Biol, 2006, 16(3): 307318.
23.	Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
24.	Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
25.	Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
26.	徐守伟, 刘晓华, 韩国庆, 等. 原发性肝癌患者外周血、肝组织中GGT mRNAH 表达及意义 [J]. 山东医药, 2009, 49(7): 6061.

1. Llovet JM, Bruix J. Novel advancements in the management of hepatocellular carcinoma in 2008 [J]. J Hepatol, 2008, 48 Suppl 1: S20S37.
2. Zhang Y, Li Q, Liu N, et al. Detection of MAGE1, MAGE3 and AFP mRNA as multimarker by realtime quantitative PCR assay: a possible predictor of hematogenous micrometastasis of hepatocellular carcinoma [J]. Hepatogastroenterology, 2008, 55(88): 22002206.
3. 钱林学, 刘玉江, 丁惠国. 小肝癌的影像学诊断进展 [J]. 世界华人消化杂志, 2010, 18(5): 479486.
4. 陈孝平, 张万广. 原发性肝癌面临的难点与对策 [J]. 中国普外基础与临床杂志， 2008, 15(4): 231233.
5. 沈啸洪, 李慧锴, 张倜. 肝癌外科规范化治疗若干问题探讨 [J]. 中国普外基础与临床杂志, 2009, 16(4): 261264.
6. Miura N, Osaki Y, Nagashima M, et al. A novel biomarker TERTmRNA is applicable for early detection of hepatoma [J]. BMC Gastroenterol, 2010, 10: 4657.
7. Wang NY, Zhang D, Zhao W, et al. Clinical application of an enzymelinked immunosorbent assay detecting hepatomaspecific γglutamyltransferase [J]. Hepatol Res, 2009, 39(10): 979987.
8. 中华医学会传染病与寄生虫病学分会、肝病学分会. 病毒性肝炎防治方案 [J]. 中华肝脏病杂志, 2000, 8(6): 324329.
9. ElSerag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis [J]. Gastroenterology, 2007, 132(7): 25572576.
10. Liu H, Li P, Zhai Y, et al. Diagnostic value of glypican3 in serum and liver for primary hepatocellular carcinoma [J]. World J Gastroenterol, 2010, 16(35): 44104415.
11. Terrin L, Rampazzo E, Pucciarelli S. et al. Relationship between tumor and plasma levels of hTERT mRNA in patients with colorectal cancer: implications for monitoring of neoplastic disease [J]. Clin Cancer Res, 2008, 14(22): 74447451.
12. Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
13. Udomchaiprasertkul W, Narong S, Kongsema M, et al. Detection of hTERT mRNA in gastrointestinal tract cancer specimens [J]. Southeast Asian J Trop Med Public Health, 2008, 39(2): 324327.
14. Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
15. Capezzone M, Marchisotta S, Cantara S, et al. Telomeres and thyroid cancer [J]. Curr Genomics, 2009, 10(8): 526533.
16. Perona R, MachadoPinilla R, Manguan C, et al. Telomerase deficiency and cancer susceptibility syndromes [J]. Clin Transl Oncol, 2009, 11(11): 711714.
17. Stewart SA, Bertuch AA. The role of telomeres and telomerase in cancer research [J]. Cancer Res, 2010, 70(19): 73657371.
18. Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells [J]. FEBS Lett, 2010, 584(17): 38193825.
19. Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
20. Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
21. Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
22. Theimer CA, Feigon J. Structure and function of telomerase RNA [J]. Curr Opin Struct Biol, 2006, 16(3): 307318.
23. Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
24. Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
25. Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
26. 徐守伟, 刘晓华, 韩国庆, 等. 原发性肝癌患者外周血、肝组织中GGT mRNAH 表达及意义 [J]. 山东医药, 2009, 49(7): 6061.

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