Effect of Genistein Combined with 5-FU on Human Gastric Cancer Cell Line SGC-7901 in Vit ro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Zhengwen ¹ , ZHU Mingcai ² , TANG Weixue ³ , GONG Jun ⁴ , FAN Weike ³ , WEI Jinlai ² , CUI Haining ¹

1. The Affiliated Hospital of Hainan Medical College, Haikou 570102 , China;;
2. Department of General Surgery , The First Affiliated Hospital , Chongqing Medical Universi t y , Chongqing 400016 , China;;
3. Department of Pathophysiology , Preclinical Medical School , Chongqing Medical Universi t y , Chongqing 400016 , China;;
4. Department of General Surgery , The People’s Hospital of Guangan , Sichuan Province , Guangan 638000 , China;

Corresponding author：

Keywords：

Gastric cancer, 　　SGC-7901 cell, 　　Genistein, 　　5-Fluorouracil, 　　Inhibitory effect

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Objective 　To study the effect of genistein (Gen) in combination with 5-fluorouracil (5-FU) on human gastric carcinoma cells in vitro. Methods 　Human gastric carcinoma cell line SGC-7901 was t reated with Gen and 5-FU alone or in combination for 24 , 48 and 72 hours , respectively. MTT assay was used to investigate the inhibitory effect of Gen and
5-FU on SGC-7901 cells. Transmission electron microscopy was used to observe the ultramicrost ructure changes of cancer cells and the flow cytometry was used to detect the distribution of cell cycle. Results 　Gen and 5-FU alone or in combination inhibited the proliferation of SGC-7901 cells significantly in both time-dependent and dose-dependent manner. The combination of Gen with 5-FU had synergistic effect on the growth of SGC-7901 cell line when the inhibition ratio was 20 % to 80 %. When SGC-7901 cells exposed to 18. 8μmol/ L Gen , 8. 84μmol/ L 52FU , and 3. 06μmol/ L Gen combined with 7. 96μmol/ L 5-FU for 72 hours , 62. 97 % , 63. 76 % and 67. 46 % SGC-7901 cells were arrested in G2 / M , S and G0 / G1 phrase , respectively. Gen and 5-FU could both induce apoptosis and arrest cell cycle of SGC-7901 cells. Conclusion 　Combination therapy of Gen with 5-FU may take synergistic inhibitory effect on the proliferation of gastric cancer cells by resting cell cycle and inducing cell apoptosis at a certain range of concent ration.

Citation： WANG Zhengwen ,ZHU Mingcai,TANG Weixue,GONG Jun,FAN Weike,WEI Jinlai,CUI Haining. Effect of Genistein Combined with 5-FU on Human Gastric Cancer Cell Line SGC-7901 in Vit ro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(1): 17-22. doi: Copy

1.	<><> [1]Barnes S.Effect of genistein on in vitro and in vivo models of cancer[J].J Nutr,1995; 125(3 Suppl)∶777S.
2.	龚军,王正文,朱明才,等.金雀异黄素和5-FU对人结肠癌细胞株(SW480)的相互作用的体外观察[J].中国现代医学杂志,2006; 16(6)∶873.
3.	龚军,王正文,朱明才,等.金雀异黄素和5-FU对人结肠癌细胞株(Colo320)的相互作用的体外观察[J].中国医师杂志,2006; 8(12)∶1601.
4.	张艳,田庆伟,李亚义.金雀异黄素对人乳癌细胞MCF-7增殖和凋亡的影响[J].天津医科大学学报,2005; 11(1)∶42.
5.	Shon YH,Park SD,Nam KS.Effective chemopreventive activity of genistein against human breast cancer cells[J].J Biochem Mol Biol,2006; 39(4)∶448.
6.	Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
7.	Bektic J,Guggenberger R,Eder IE,et al.Molecular effects of the isoflavonoid genistein in prostate cancer[J].Clin Prostate Cancer,2005; 4(2)∶124.
8.	Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
9.	韩锐主编.抗癌药物研究与实验技术[M].北京:北京医科大学中国协和医科大学联合出版社,1997∶399～400.
10.	Shao ZM,Wu J,Shen ZZ,et al.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Cancer Res,1998; 58(21)∶4851.
11.	Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
12.	Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
13.	Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
14.	Ravindranath MH,Muthugounder S,Presser N,et al.Anticancer therapeutic potential of soy isoflavone,genistein[J].Adv Exp Med Biol,2004; 546∶121.
15.	Sarkar FH,Adsule S,Padhye S,et al.The role of genistein and synthetic derivatives of isoflavone in cancer prevention and therapy[J].Mini Rev Med Chem,2006; 6(4)∶401.
16.	金懋林.进展期胃癌全身化学治疗新进展[J].外科理论与实践,2003; 8(1)∶18.
17.	刘福坤,姚学权.胃癌的新辅助化疗[J].中国普外基础与临床杂志,2006; 13(1)∶17.
18.	黄斌,陈志新.胃癌淋巴靶向化疗的研究进展[J].中国普外基础与临床杂志,2006; 13(5)∶ 617.
19.	Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
20.	Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
21.	Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
22.	McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
23.	Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
24.	Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
25.	叶正宝,马韬.胃肠道癌瘤的化学治疗[A].见:朱正纲主编.胃肠道肿瘤外科综合治疗新技术[M].北京:人民军医出版社,2002∶154～180.

1. <><> [1]Barnes S.Effect of genistein on in vitro and in vivo models of cancer[J].J Nutr,1995; 125(3 Suppl)∶777S.
2. 龚军,王正文,朱明才,等.金雀异黄素和5-FU对人结肠癌细胞株(SW480)的相互作用的体外观察[J].中国现代医学杂志,2006; 16(6)∶873.
3. 龚军,王正文,朱明才,等.金雀异黄素和5-FU对人结肠癌细胞株(Colo320)的相互作用的体外观察[J].中国医师杂志,2006; 8(12)∶1601.
4. 张艳,田庆伟,李亚义.金雀异黄素对人乳癌细胞MCF-7增殖和凋亡的影响[J].天津医科大学学报,2005; 11(1)∶42.
5. Shon YH,Park SD,Nam KS.Effective chemopreventive activity of genistein against human breast cancer cells[J].J Biochem Mol Biol,2006; 39(4)∶448.
6. Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
7. Bektic J,Guggenberger R,Eder IE,et al.Molecular effects of the isoflavonoid genistein in prostate cancer[J].Clin Prostate Cancer,2005; 4(2)∶124.
8. Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
9. 韩锐主编.抗癌药物研究与实验技术[M].北京:北京医科大学中国协和医科大学联合出版社,1997∶399～400.
10. Shao ZM,Wu J,Shen ZZ,et al.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Cancer Res,1998; 58(21)∶4851.
11. Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
12. Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
13. Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
14. Ravindranath MH,Muthugounder S,Presser N,et al.Anticancer therapeutic potential of soy isoflavone,genistein[J].Adv Exp Med Biol,2004; 546∶121.
15. Sarkar FH,Adsule S,Padhye S,et al.The role of genistein and synthetic derivatives of isoflavone in cancer prevention and therapy[J].Mini Rev Med Chem,2006; 6(4)∶401.
16. 金懋林.进展期胃癌全身化学治疗新进展[J].外科理论与实践,2003; 8(1)∶18.
17. 刘福坤,姚学权.胃癌的新辅助化疗[J].中国普外基础与临床杂志,2006; 13(1)∶17.
18. 黄斌,陈志新.胃癌淋巴靶向化疗的研究进展[J].中国普外基础与临床杂志,2006; 13(5)∶ 617.
19. Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
20. Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
21. Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
22. McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
23. Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
24. Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
25. 叶正宝,马韬.胃肠道癌瘤的化学治疗[A].见:朱正纲主编.胃肠道肿瘤外科综合治疗新技术[M].北京:人民军医出版社,2002∶154～180.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Genistein Combined with 5-FU on Human Gastric Cancer Cell Line SGC-7901 in Vit ro

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