Expression of T Cell Costimulatory Molecule and Variance of T Cell Subpopulations in Patients with Gastric Cancer andColorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

FU Lengx i ¹ , ZHANG Sheng ² , CHEN Sizeng ³

1. Central Laboratory ;;
2. Department of Pathology ;;
3. Department of Oncology ;;
The First Affiliated Hospital , Fujian Medical University , Fuzhou 350005 , China;

Corresponding author：

Keywords：

Gast ric cancer,　Colorectal cancer,　Costimulatory molecule,　T cell subpopulations, Flow cytometry

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Objective 　To investigate the role of expression of T cell costimulatory molecule CD28 and variance of T cell subpopulations in the development and prognosis of gastric cancer and colorectal cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule CD28 by flow cytometry in 38 patients with gastric cancer, 42 patient s with colorectal cancer , and 21 healthy peoples as control group . Results 　Expressions of T cell costimulatory molecule CD28 in patients with gastric cancer and colorectal cancer were (25. 80 ±10. 56) % and (28. 95 ±9. 29) % , and significantly higher than that of control group 〔(0. 82 ±0. 98) % , P lt; 0. 01〕. Expression percentage of total T cell (CD3 + ) in patient s with gastric cancer and colorectal cancer were significantly lower than that of control group 〔(53. 61 ±13. 84) % and (55. 96 ±10. 68) % vs (72. 07 ±7. 83) % , P lt; 0. 01〕. Expression percentage of CD4 + T cell (CD4 + CD3 + ) in patients with gastric cancer and colorectal cancer were significantly lower than that of control group 〔( 29. 84 ±9. 71) % and ( 33. 75 ±9. 04) % vs (38. 79 ±5. 08) %; P lt; 0. 01 , P lt; 0. 05〕; Expression percentage of CTL cell (CD8 + CD28 + CD3 + ) in patient s with gastric cancer and colorectal cancer were significantly higher than that of control group 〔( 1. 57 ±1. 99) % and (1. 93 ±2. 61) % vs (0. 02 ±0. 04) %; P lt; 0. 01〕; Expression percentage of CD8 + inhibitory T cell (CD8 + CD28 -CD 3 + ) and CD4 / CD8 ratio in patient s with gastric cancer were significantly lower than that of control group 〔(16. 06 ±6. 94) % vs (20. 56 ±6. 54) % , P lt; 0. 05 ; (1. 10 ±0. 51) % vs (1. 36 ±0. 31) % , P lt; 0. 05〕; Expression of regulatory T cell (CD4 + CD25 + CD3 + ) of patients with colorectal cancer was (19. 74 ±6. 89) % , which was significantly higher than that of control group 〔(13. 72 ±3. 08) % , P lt; 0. 01〕. No difference of expression was found in peripheral T cell subpopulations of postoperative patients with gastric cancer and colorectal cancer after one week ( P gt; 0. 05) . Conclusion 　T cell number is fall ,T cell costimulatory molecule CD28 useless expression is increase in patient s with gastric cancer and colorectal cancer. CD4 + T cell subpopulation is significantly decreased in patient s with gast ric cancer. The regulatory T cell of patient s with colorectal cancer is significantly increased.

Citation： FU Lengx i ,ZHANG Sheng,CHEN Sizeng. Expression of T Cell Costimulatory Molecule and Variance of T Cell Subpopulations in Patients with Gastric Cancer andColorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2008, 15(1): 51-55. doi: Copy

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1. Antony PA,Piccirillo CA,Akpinarli A,et al.CD8+ T cell immunity against a tumor/self-antigen is augmented by CD4+ T helper cells and hindered by naturally occurring T regulatory cells[J].J Immunol,2005; 174(5)∶2591.
2. 林学颜,张玲主编.现代细胞分子免疫学[M].第1版.北京:科学出版社,2003∶384～385.
3. Sakaguchi S,Sakaguchi N,Asano M,et al.Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25).Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases[J].J Immunol,1995; 155(3)∶1151.
4. Chattopadhyay S,Chakraborty NG,Mukherji B.Regulatory T cells and tumor immunity[J].Cancer Immunol Immunother,2005; 54(12)∶1153.
5. 吴静静,王俐.CD4+CD25+ 调节性T 细胞及其与肿瘤的关系[J].国外医学·肿瘤学分册,2004; 31(7)∶496.
6. 李晓,汪辉.CD4+CD25+调节性T细胞和肿瘤免疫[J].国外医学·免疫学分册,2003; 26(6)∶326.
7. Yu P,Lee Y,Liu W,et al.Intratumor depletion of CD4+ cells unmasks tumor immunogenicity leading to the rejection of late-stage tumors[J].J Exp Med,2005; 201(5)∶779.
8. Seo N,Hayakawa S,Takigawa M,et al.Interleukin-10 expressed at early tumour sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of antitumour immunity[J].Immunology,2001; 103(4)∶449.
9. 刘莉,丁乾,姚军霞,等.结直肠癌患者外周血CD4+CD25high调节性T细胞分析[J].中华肿瘤防治杂志,2007; 14(2)∶110.
10. 任秀红,刘莉,刘平平,等.恶性肿瘤患者T细胞亚群变化及其与肿瘤分期的关系[J].第三军医大学学报,2006; 28(18)∶1906.
11. Ercolini AM,Ladle BH,Manning EA,et al.Recruitment of latent pools of high-avidity CD8(+) T cells to the antitumor immune response[J].J Exp Med,2005; 201(10)∶1591.
12. Sasada T,Kimura M,Yoshida Y,et al.CD4+CD25+ regulatory T cells in patients with gastrointestinal malignancies:possible involvement of regulatory T cells in disease progression[J].Cancer,2003; 98(5)∶1089.
13. Scotto L,Naiyer AJ,Galluzzo S,et al.Overlap between molecular markers expressed by naturally occurring CD4+CD25+ regulatory T cells and antigen specific CD4+CD25+ and CD8+CD28-T suppressor cells[J].Hum Immunol,2004; 65(11)∶1297.
14. 金伯泉主编.细胞和分子免疫学[M].第2版.北京:科学出版社,2001∶17.
15. 林文棠,朱平主编.实用临床免疫学[M].第1版.西安:第四军医大学出版社,2003∶166～168.
16. 李晓,徐兰波.共刺激分子在肿瘤免疫治疗中的应用[J].国外医学·肿瘤学分册,2005; 32(1)∶25.
17. 张晓膺,郑世营,赵军,等.外源性B7-1基因诱导抗肺癌主动免疫的实验研究[J].中华实验外科杂志,2005; 22(7)∶884.
18. 张清媛,李殿俊,王志华,等.B7-1基因修饰的肿瘤细胞疫苗抗肿瘤的实验研究[J].中国免疫学杂志,2001; 17(5)∶249.
19. 陈农.胃癌患者手术前后外周血自然杀伤细胞和T淋巴细胞亚群的变化[J].浙江医学,2002; 24(10)∶579.
20. 董晖,王朝杰.结直肠癌患者免疫状况与肿瘤分期的关系[J].国外医学·放射医学核医学分册,2005; 29(3)∶115.
21. 陈荣,蔡景理,周斌,等.免疫增强型肠内营养制剂对结直肠肿瘤术后机体免疫的影响[J].中华胃肠外科杂志,2005; 8(4)∶328.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of T Cell Costimulatory Molecule and Variance of T Cell Subpopulations in Patients with Gastric Cancer andColorectal Cancer

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