Effect of Bile from Patients with Anomalous Pancreaticobiliary Ductal Union on Growth of Human Cells of Bile Duct_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Lei ¹ , ZHANG Bin ¹ , LI Changfeng ¹ , LI Dandan ¹ , SUN Libo ² , ZHENG Zelin ²

Department of Endoscopy, The China-Japan Union Hospital of Jilin Uninversity, Changchun 130033, China;

Corresponding author：

Keywords：

Anomalous pancreaticobiliary ductal union; Bile; Tumor of bile duct

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Objective 　 To explore the effects of bile from anomalous parcreaticobiliary ductal union (APBDU) patients on the growth of hunan cells of bile ducts, and the relationship between APBDU with bile duct carcinoma.
Methods 　Bile sample from APBDU patients and normal persons were used for cells of bile ducts. The proliferative effect of bile was measured by mathabenzthiazuron (MTT) assay; Cell cycle and apoptosis were analyzed by flow cytometry (FCM); Expression level of c-erbB-2 was detected by Western blot assay.
Results 　Bile from APBDU patients significantly promoted the proliferation of human cells of bile ducts compared with normal bile （P＜0.05）. The effect was abolished by addition of COX-2 inhibitor or iNOS inhibitor. The percentage of S period cells in group pretreated with APBDU bile was significantly increased compared with pretreated with normal bile (P＜0.001). The expression level of c-erbB-2 was increased in cell pretreated with APBDU bile.
Conclusion 　Bile from APBDU patients can promote the proliferation of normal cells of bile ducts and may have potential carcinogenesis.

Citation： YANG Lei,ZHANG Bin,LI Changfeng,LI Dandan,SUN Libo,ZHENG Zelin. Effect of Bile from Patients with Anomalous Pancreaticobiliary Ductal Union on Growth of Human Cells of Bile Duct. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 648-651. doi: Copy

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1. 　孟翔凌，何磊，徐阿曼, 等. 胰胆管合流异常的临床意义 [J]. 中国普外基础与临床杂志， 2003; 10（5）∶451.
2. 　许圣献，单礼成，范西红, 等. 先天性胰胆管合流异常20例报告 [J]. 中国普外基础与临床杂志, 1998; 5(6)∶371.
3. 　张克俭，于忠，王运新，等. 先天性胆管囊性扩张合并胰胆管合流异常 [J]. 中华消化内镜杂志， 2000； 17(2)∶78.
4. 　段体德. 胰胆管合流异常并发胆胰疾病的诊治问题 [J]. 中国普外基础与临床杂志, 1999; 5(2)∶111.
5. 　Kimura K, Ohto M, Saisho H, et al. Association of gallbladder carcinoma and anomalous pancreaticobiliary ductal union [J]. Gastroenterology, 1985; 89(6)∶1258.
6. 　Takayashiki T, Miyazaki M, Kato A, et al. Double cancer of gallbladder and bile duct associated with anomalous junction of the pancreaticobiliary ductal system [J]. Hepatogastroenterology, 2002; 49(43)∶109.
7. 　王亚东，王作仁，王琳，等. 胰胆管合流异常与胆囊癌 [J]. 中华肝胆外科杂志， 1998； 4（1）∶31.
8. 　Yoon JH, Higuchi H, Werneburg NW, et al. Bile acids induce cyclooxygenase-2 expression via the epidermal growth factor receptor in a human cholangiocarcinoma cell line [J]. Gastroenterology, 2002; 122(4)∶985.
9. 　Tanno S, Obara T, Fujii T, et al. Proliferative potential and K-ras mutation in epithelial hyperplasia of the gallbladder in patients with anomalous pancreaticobiliary ductal union [J]. Cancer, 1998; 83(2)∶267.
10. 　Qian D, Kinouchi T, Kunitomo K, et al. Mutagenicity of the bile of dogs with an experimental model of an anomalous arrangement of the pancreaticobiliary duct [J]. Carcinogenesis, 1993; 14(4)∶743.
11. 　Jaiswal M, LaRusso NF, Shapiro RA, et al. Nitric oxide-mediated inhibition of DNA repair potentiates oxidative DNA damage in cholangiocytes [J]. Gastroenterology, 2001; 120(1)∶190.
12. 　Spirlì C, Fabris L, Duner E, et al. Cytokine-stimulated nitric oxide production inhibits adenylyl cyclase and cAMP-dependent secretion in cholangiocytes [J]. Gastroenterology, 2003; 124(3)∶737.
13. 　董倩，杨波，江布先，等. PCNA与p53蛋白在先天性胆管扩张症患者胆管中的表达及意义 [J]. 山东医药， 2003； 43（21）∶3.
14. 　Tokiwa K, Ono S, Iwai N. Mucosal cell proliferation activity of the gallbladder in children with anomalous arrangement of the pancreaticobiliary duct [J]. J Hepatobiliary Pancreat Surg, 1999; 6(3)∶213.
15. 　Nagai M, Watanabe M, Iwase T, et al. Clinical and genetic analysis of noncancerous and cancerous biliary epithelium in patients with pancreaticobiliary maljunction [J]. World J Surg, 2002; 26(1)∶91.
16. 　梁平，陈海，庄大勇，等. PCNA和bcl-2 在金仓鼠胆管癌和胆管细胞增生中的表达 [J]. 第三军医大学学报， 2001； 23（7）∶790.
17. 　Richter M, Zhang H. Receptor-targeted cancer therapy [J]. DNA Cell Biol, 2005; 24(5)∶271.
18. 　Kimura K, Ohto M, Saisho H, et al. Association of gallbladder carcinoma and anomalous pancreaticobiliary ductal union [J]. Gastroenterology, 1985; 89(6)∶1258.
19. 　郑军，朱耀明，邹声泉，等. 原癌基因c-erbB-2在胆管癌细胞中的表达 [J]. 实用医学进修杂志， 2006； 34（2）∶79.
20. 　Nakazawa K,Dobashi Y,Suzuki S,et al.Amplification and overexpression of c-erbB-2,epidermal growth factor receptor,and c-met in biliary tract cancers [J].J Pathol,2005;206(3)∶356.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Bile from Patients with Anomalous Pancreaticobiliary Ductal Union on Growth of Human Cells of Bile Duct

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