Mutation and Expression of β-Catenin Gene in Breast Hyperplasia and Breast Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Zhen ¹ , CUI Dongxu ¹ , LIU Baolin ¹ , MA Wenfeng ² , ZHANG Qiang ²

The First Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China;

Corresponding author：

Keywords：

Breast neoplasms; β-catenin; Gene; Polymerase chain reaction; Immunohistochemistry

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the role of β-catenin gene in breast tumorigenesis by detecting mutation and expression of β-catenin gene in breast hyperplasia and breast cancer.
Methods 　Mutation and expression of β-catenin gene in 42 breast cancer, 15 simple hyperplasia and 15 atypical hyperplasia were detected by polymerase chain reaction-single strand conformation polymorphism and immunohistochemistry.
Results 　Normal expression of β-catenin occurred in tissue of breast simple hyperplasia. The rate of abnormal expression of β-catenin in tissue of breast atypical hyperplasia and breast cancer were 26.7% （4/15） and 59.5% （25/42）, respectively, which were higher than that of simple hyherplasia tissue (P＜0.05). And there was a markedly difference between the atypical hyperplasia tissue and breast cancer tissue (P＜0.05). Mutation of β-catenin gene wasn’t detected in this three kinds of tissues.
Conclusion 　Abnormal expression of β-catenin plays an important role in human breast tumorigenesis, reason of abnormal expression of β-catenin isn’t mutation of β-catenin gene. Expression of β-catenin can be regulated by other mechanisms.

Citation： LIU Zhen,CUI Dongxu,LIU Baolin,MA Wenfeng,ZHANG Qiang. Mutation and Expression of β-Catenin Gene in Breast Hyperplasia and Breast Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(6): 685-688. doi: Copy

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1. 　刘臻，张强，马文锋, 等. 上皮钙黏蛋白和α-连接素的表达与乳腺癌生物学特性的关系 [J]. 中国普外基础与临床杂志, 2005; 12（3）∶226.
2. 　刘臻, 张强, 马文锋, 等. 上皮钙黏蛋白、 α-连环素和β-连环素在乳腺癌中的表达及意义 [J]. 中华外科杂志, 2005; 43(5)∶307.
3. 　Chang HJ, Jee CD, Kim WH. Mutation and altered expression of beta-catenin during gallbladder carcinogenesis [J]. Am J Surg Pathol, 2002; 26(6)∶758.
4. 　Rashid A, Gao YT, Bhakta S, et al. Beta-catenin mutations in biliary tract cancers: a population-based study in China [J]. Cancer Res, 2001; 61(8)∶3406.
5. 　Park WS, Oh RR, Park JY, et al. Frequent somatic mutations of the beta-catenin gene in intestinal-type gastric cancer [J]. Cancer Res, 1999; 59(17)∶4257.
6. 　董泾青, 沈祖楹, 于扬, 等. β-catenin和APC蛋白在甲状腺肿瘤中的表达及意义 [J]. 贵阳医学院学报, 2002; 27(6)∶471.
7. 　Muller T, Choidas A, Reichmann E, et al. Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration [J]. J Biol Chem, 1999; 274(15)∶10173.
8. 　Karim R, Tse G, Putti T, et al. The significance of the Wnt pathway in the pathology of human cancers [J]. Pathology, 2004; 36(2)∶120.
9. 　Fearnhead NS, Britton MP, Bodmer WF. The ABC of APC [J]. Hum Mol Genet, 2001; 10(7)∶721.
10. 　Bankfalvi A, Terpe HJ, Breukelmann D, et al. Immunophenotypic and prognostic analysis of E-cadherin and beta-catenin expression during breast carcinogenesis and tumour progression: a comparative study with CD44 [J]. Histopathology, 1999; 34(1)∶25.
11. 　Michaelson JS, Leder P. beta-catenin is a downstream effector of Wnt-mediated tumorigenesis in the mammary gland [J]. Oncogene, 2001; 20(37)∶5093.
12. 　Imbert A, Eelkema R, Jordan S, et al. Delta N89 beta-catenin induces precocious development, differentiation, and neoplasia in mammary gland [J]. J Cell Biol, 2001; 153(3)∶555.
13. 　Miyaki M, Iijima T, Kimura J, et al. Frequent mutation of beta-catenin and APC genes in primary colorectal tumors from patients with hereditary nonpolyposis colorectal cancer [J]. Cancer Res, 1999; 59(18)∶4506.
14. 　Miyoshi Y, Iwao K, Nagasawa Y, et al. Activation of the beta-catenin gene in primary hepatocellular carcinomas by somatic alterations involving exon 3 [J]. Cancer Res, 1998; 58(12)∶2524.
15. 　Fukuchi T, Sakamoto M, Tsuda H, et al. Beta-catenin mutation in carcinoma of the uterine endometrium [J]. Cancer Res, 1998; 58(16)∶3526.
16. 　Palacios, Gamallo C. Mutations in the β-catenin gene (CTNNB1) in endometrioid ovarian carcinomas [J]. Cancer Res, 1998; 58(7)∶1344.
17. 　Voeller HJ, Truica CI, Gelmann EP. Beta-catenin mutations in prostate cancer [J]. Cancer Res, 1998; 58(12)∶2520.
18. 　Zhuang SM, Wiseman RW, Soderkvist P. et al. Frequent mutations of the Trp53, Hras1 and beta-catenin (Catnb) genes in 1, 3-butadiene-induced mammary adenocarcinomas in B6C3F1 mice [J]. Oncogene, 2002; 21(36)∶5643.
19. 　Jonsson M, Borg A, Nilbert M, et al. Involvement of adenomatous polyposis coli (APC)/beta-catenin signalling in human breast cancer [J]. Eur J Cancer, 2000; 36(2)∶242.
20. 　Suriano G, Vrcelj N, Senz J, et al. beta-catenin (CTNNB1) gene amplification: a new mechanism of protein overexpression in cancer [J]. Genes Chromosomes Cancer, 2005; 42(3)∶238.
21. 　Mao J, Wang J, Liu B, et al. Low-density lipoprotein receptor-related protein-5 binds to Axin and regulates the canonical Wnt signaling pathway [J]. Mol Cell, 2001; 7(4)∶801.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Mutation and Expression of β-Catenin Gene in Breast Hyperplasia and Breast Cancer

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