Effect of RNA Interference on Endogenous bcl-2 Expression of Vascular Smooth Muscle Cells in Rabbit_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Hua ¹ , JING Zaiping ² , BAO Junmin ² , QU Lefeng ² , FENG Xiang ² , CHENG Xiaoxing ³ , YANG Zhong ³

Department of General Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038， China;

Corresponding author：

Keywords：

RNA interference; Vascular smooth muscle cell; bcl-2 gene

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Objective　To study the inhibitory effect of RNA interference (RNAi) on bcl-2 expression of vascular smooth muscle cells （VSMCs) in rabbit.
Methods　The expression vector of bcl-2 gene-targeting small interference RNA (pshRNA-bcl-2) was constructed and was transfected into VSMCs by lipofectamine, and the unloaded vector was used as control. The expression of bcl-2 mRNA was identified by RT-PCR and Western blot, respectively. The growth of the transfected VSMCs was examined by MTT.
Results　The pshRNA-bcl-2 may inhibit the expression of bcl-2 gene at the levels of transcription and translation. There were significant differences (P＜0.01) of the expressions of bcl-2 mRNA between the VSMCs that were transfected with pshRNA-bcl-2 and the ones in plasmid transfected group and control group, respectively. There was a significant difference (P＜0.01) in the growth of VSMCs between the plasmid transfected and the control groups.
Conclusion　The plasmid containing the small interference RNA of bcl-2 may have an inhibitory effect on the cell growth and endogenous expression of bcl-2 gene at the levels of transcription and translation in VSMCs.

Citation： CHEN Hua,JING Zaiping,BAO Junmin,QU Lefeng,FENG Xiang,CHENG Xiaoxing,YANG Zhong. Effect of RNA Interference on Endogenous bcl-2 Expression of Vascular Smooth Muscle Cells in Rabbit. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 56-58. doi: Copy

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9.	冯勇, 胡海地, 段志泉, 等. 凋亡相关基因bcl-2和bax在大鼠自体移植静脉中的动态表达及意义 [J]. 中国普外基础与临床杂志， 2004； 11（4）∶295.

1.
2. 　Aoki Y, Cioca D, Oidaira H, et al. RNA interference may be more potent than antisense RNA in human cancer cell lines [J]. Clin Exp Pharmacol Physiol, 2003; 30(1-2)∶96.
3. 　Zhao Z, Huang C, Wang J, et al. Effect of arsenic trioxide on inhibition of restenosis after rabbit vascular injury and its mechanism [J]. Chin Med J (Engl), 2002; 115(11)∶1608.
4. 　蹇锐, 程小星, 安静, 等. 应用载体介导的RNAi技术抑制Bcl-2的表达 [J]. 第三军医大学学报， 2004； 26（4）∶294.
5. 　周晓莉，雷寒，柳青. 小鼠主动脉平滑肌细胞的培养 [J]. 基础医学与临床， 2005； 25（6）∶564.
6. 　Caplen NJ, Parrish S, Imani F, et al. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J]. Proc Natl Acad Sci USA, 2001; 98(17)∶9742.
7. 　Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J]. Nature, 2001; 411(6836)∶494.
8. 　Jabs A, Kramer S, Skowasch D, et al. Neointimal hyperplasia by luminal cell recruitment and not be transmural migration. The role of Bcl-2 and HSP47 after balloon angioplasty [J]. Z Kardiol, 2002; 91(8)∶626.
9. 　冯勇, 胡海地, 段志泉, 等. 凋亡相关基因bcl-2和bax在大鼠自体移植静脉中的动态表达及意义 [J]. 中国普外基础与临床杂志， 2004； 11（4）∶295.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of RNA Interference on Endogenous bcl-2 Expression of Vascular Smooth Muscle Cells in Rabbit

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