Effects of Nimesulide on Expression of Matrix Metalloproteinase- 2 in Human Colonic Cancer Cell Lines_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Wei ¹ , AN Jie ² , ZHANG Chao. ¹

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Keywords：

Colonic neoplasm Cyclooxygenase Inhibitor Matrix metalloproteinase

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【Abstract】Objective To investigate the effects of selective cyclooxygenase-2 (COX-2) inhibitor nimesulide on the proliferation of colon adenocarcinoma cells in vitro and the expression of matrix metalloproteinase-2 (MMP-2). Methods The human colon cancer cell lines HT-29 and HCT-116 were employed in the study, grouped as nimesulide group, DMSO control group and blank control group. After treatment with nimesulide, the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay, and the expression of MMP-2 in the cells was detected by quantitative zymography. Results Nimesulide inhibited the proliferation of HT-29 and HCT-116 cells in time and dosedependent manners. The inhibitory effect on HT-29 cells was ber than that on HCT-116 cells. Nimesulide downregulated the MMP-2 expression in HT-29 cells, whereas the expression in HCT-116 cells remained unchanged.

Conclusion Nimesulide can obviously inhibit the growth of colon cancer HT-29 cells with positive COX-2 protein, suggesting that nimesulide may downregulate the expression of MMP-2 by inhibiting the activity of COX-2.

Citation： LIU Wei,AN Jie,ZHANG Chao.. Effects of Nimesulide on Expression of Matrix Metalloproteinase- 2 in Human Colonic Cancer Cell Lines. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(6): 585-588. doi: Copy

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1. 1 Fujita T, Matsui M, Takaku K, et al. Size  and invasiondependent increase in cyclooxygenase 2 levels in human colorectal carcinomas [Ｊ]. Cancer Res， 1998; 58(21)48232 Mook OR, Frederiks WM, Van Noorden CJ. The role of gelatinases in colorectal cancer progression and metastasis [Ｊ]. Biochim Biophys Acta, 2004; 1705(2)693 Reddy BS, Rao CV. Colon cancer: a role for cyclooxygenase2specific nonsteroidal antiinflammatory drugs [Ｊ]. Drugs Aging, 2000; 16(5)3294 Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2 [Ｊ]. Cell, 1995; 83(3)4935 Piazza GA, Rahm AK, Finn TS, et al. Apoptosis primarily accounts for the growthinhibitory properties of sulindac metabolites and involves a mechanism that is independent of cyclooxygenase inhibition, cell cycle arrest, and p53 induction [Ｊ]. Cancer Res, 1997; 57(12)24526 Shiff SJ, Qiao L, Tsai LL, et al. Sulindac sulfide, an aspirinlike compound, inhibits proliferation, causes cell cycle quiescence, and induces apoptosis in HT29 colon adenocarcinoma cells [Ｊ]. J Clin Invest, 1995; 96(1)4917 Kleiner DE, StetlerStevenson WG. Matrix metalloproteinases and metastasis [Ｊ]. Cancer Chemother Pharmacol, 1999; 43(Suppl)S428 Wu J, Akaike T, Hayashida K, et al. Enhanced vascular permeability in solid tumor involving peroxynitrite and matrix metalloproteinases [Ｊ]. Jpn J Cancer Res, 2001; 92(4)4399 Oba K, Konno H, Tanaka T, et al. Prevention of liver metastasis of human colon cancer by selective matrix metalloproteinase inhibitor MMI166 [Ｊ]. Cancer Lett, 2002; 175(1)4510 Papadopoulou S, Scorilas A, Arnogianaki N, et al. Expression of gelatinaseA (MMP2) in human colon cancer and normal colon mucosa [Ｊ]. Tumour Biol, 2001; 22(6)38311 Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblastderived matrix metalloproteinase2 by coloncancer cells in noncontact Cocultures [Ｊ]. Int J Cancer, 2000; 87(2)16512 Li G, Yang T, Yan J. Cyclooxygenase-2 increased the angiogenic and metastatic potential of tumor cells [Ｊ]. Biochem Biophys Res Commun， 2002; 299(5)886.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Nimesulide on Expression of Matrix Metalloproteinase- 2 in Human Colonic Cancer Cell Lines

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