【Abstract】Objective To investigate the irradiating effect of low intensive microwave (LIM) on pathological process of blood vessel restenosis(RS) and assess the probability of LIM irradiation to prevent was used RS.Methods Fortyfour male healthy New Zealand rabbits were randomly divided into 2 groups. Fogarty catheter traumatize to the tunica intima of iliac artery so as to establish RS models. Two thousand four hundred and fifty MHz microwave with different power of 2 ,5 and 10 mW/cm2 was used, locally to irradiate EIA in irradiating group (1 h/d). Specimens were obtained at different time of 3,7,14 and 28 d after operation. Morphological changes of tissues were observed with HE and EF staining and the area of tunica intima, tunica media and the rate of cavity stenosis were analyzed with image analysis system; apoptosis was detected with TUNEL; phenotype and microstructure of VSMC were observed with TEM. Results After microwave irradiating, inflammatory reaction in early period was suppressed, mural thrombus decreased, the proliferation and migration of VSMC depressed, the area of tunica intima and the rate of cavity stenosis obviously reduced comparing with the control group (P＜0.01). The rate of apoptosis cells showed that there were no obvious differences among each group on 3 d after operation (P gt;0.05). At other different time, however, the rate of apoptosis cells in irradiating groups obviously increased than that of the control group (P＜0.01), particularly in the one with power of 5 mW/cm2 .The number of synthesis form VSMC in the control group occupied (93.50±3.45)% of the total number of VSMC on 14 d after operation. Most of VSMC appear contractile in irradiating group in which a lot of morphological changes of apoptosis in fibroblast and VSMC existed.Conclusion LIM irradiation could obviously prevented from pathologic procedure of RS. After LIM irradiating, inflammatory reaction in early period is suppressed, the proliferation and migration of VSMC depressed. LIM irradiation promotes cell apoptosis, effectively prohibites the occurring and development of RS. LIM irradiation has had relationship between quantity and effect, power span to effectively prohibit RS, particularly in the one with power of 5 mW/cm2.
Citation：
WANG Zhigang,LEI Zehua,SHI De,QIAO Zhengrong.. Irradiating Effect of Low Intensive Microwave on Restenosis of External Iliac Artery of Rabbit after Injury. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(6): 595-599. doi:
Copy
| 1. |
1 Ferns GA, Avades TY. The mechanisms of coronary restenosis: insights from experimental models
[Ｊ]. Int J Exp Pathol， 2000; 81(2)632 Kolosova LI, Akoev GN, Avelev VD, et al. Effect of lowintensity millimeter wave electromagnetic radiation on regeneration of the sciatic nerve in rats [Ｊ]. Bioelectromagnetics， 1996; 17(1)443 杜忠民，郭鹞. 毫米波辐射对小鼠EMT6乳腺癌细胞增殖活性的影响 [Ｊ]. 中国病理生理杂志， 1994; 10（1）404 王保义，王长广，王登本,等. 低强度微波辐射对人精子非热生物效应的研究 [Ｊ]. 生物物理学报，1996; 12（1）1395 Lentz RR, Stensaas LJ, Partlow LM. Effects of millimeterwave radiation on monolayer cell cultures [Ｊ]. J microwave Power, 1990; 15(4)1136 Nossol B, Buse G, Silny J. Influence of weak static and 50 Hz magnetic fields on the redox activity of cytochromeC oxidase [Ｊ]. Bioelectromagnetics, 1993; 14(4)3617 Wen JK, Shi Y. Progress in the study of mechanisms regulating vascular smooth muscle cell proliferation [Ｊ]. Sheng Li Ke Xue Jin Zhan, 1996; 27(2)1498 Peinnequin A, Piriou A, Mathieu J, et al. Nonthermal effects of continuous 2.45 GHz microwaves on Fasinduced apoptosis in human Jurkat Tcell line [Ｊ]. Bioelectrochemistry, 2000; 51(2)1579 Lai H, Singh NP. Acute lowintensity microwave exposure increases DNA singlestrand breaks in rat brain cells [Ｊ]. Bioelectromagnetics, 1995; 16(3)20710 Singh N, Rudra N, Bansal P, et al. Poly ADP ribosylation as a possible mechanism of microwave——biointeraction [Ｊ]. Indian J Physiol Pharmacol， 1994; 38(3)18111 Lai H, Singh NP. Melatonin and a spintrap compound block radiofrequency electromagnetic radiationinduced DNA strand breaks in rat brain cells [Ｊ]. Bioelectromagnetics， 1997; 18(6)44612 Harvey C, French PW. Effects on protein kinase C and gene expression in a human mast cell line, HMC1, following microwave exposure [Ｊ]. Cell Biol Int， 2000; 23(11)739.
|
- 1. 1 Ferns GA, Avades TY. The mechanisms of coronary restenosis: insights from experimental models
[Ｊ]. Int J Exp Pathol， 2000; 81(2)632 Kolosova LI, Akoev GN, Avelev VD, et al. Effect of lowintensity millimeter wave electromagnetic radiation on regeneration of the sciatic nerve in rats [Ｊ]. Bioelectromagnetics， 1996; 17(1)443 杜忠民，郭鹞. 毫米波辐射对小鼠EMT6乳腺癌细胞增殖活性的影响 [Ｊ]. 中国病理生理杂志， 1994; 10（1）404 王保义，王长广，王登本,等. 低强度微波辐射对人精子非热生物效应的研究 [Ｊ]. 生物物理学报，1996; 12（1）1395 Lentz RR, Stensaas LJ, Partlow LM. Effects of millimeterwave radiation on monolayer cell cultures [Ｊ]. J microwave Power, 1990; 15(4)1136 Nossol B, Buse G, Silny J. Influence of weak static and 50 Hz magnetic fields on the redox activity of cytochromeC oxidase [Ｊ]. Bioelectromagnetics, 1993; 14(4)3617 Wen JK, Shi Y. Progress in the study of mechanisms regulating vascular smooth muscle cell proliferation [Ｊ]. Sheng Li Ke Xue Jin Zhan, 1996; 27(2)1498 Peinnequin A, Piriou A, Mathieu J, et al. Nonthermal effects of continuous 2.45 GHz microwaves on Fasinduced apoptosis in human Jurkat Tcell line [Ｊ]. Bioelectrochemistry, 2000; 51(2)1579 Lai H, Singh NP. Acute lowintensity microwave exposure increases DNA singlestrand breaks in rat brain cells [Ｊ]. Bioelectromagnetics, 1995; 16(3)20710 Singh N, Rudra N, Bansal P, et al. Poly ADP ribosylation as a possible mechanism of microwave——biointeraction [Ｊ]. Indian J Physiol Pharmacol， 1994; 38(3)18111 Lai H, Singh NP. Melatonin and a spintrap compound block radiofrequency electromagnetic radiationinduced DNA strand breaks in rat brain cells [Ｊ]. Bioelectromagnetics， 1997; 18(6)44612 Harvey C, French PW. Effects on protein kinase C and gene expression in a human mast cell line, HMC1, following microwave exposure [Ｊ]. Cell Biol Int， 2000; 23(11)739.
