Effects of DNA Electrotransfer in Muscle on the Implanted Tumor Growth in Nude Mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SUN Hui , WANG Jinguo , FU Yantao , LUAN Shan , ZHENG Zelin.

Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033,China;

Corresponding author：

Keywords：

DNA electrotransfer in muscle Human anaplastic thyroid cancer cell line TA-K Gene therapy

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】ObjectiveTo study the antitumor effects of DNA electrotransfer in muscle (ETM) by using established animal model for human anaplastic thyroid cancer cell line TA-K.
MethodsNude mice with implanted TA-K were divided into five groups including: control group, pcDNA-3 plasmid electrotransfered into muscle (pcDNA ETM group), TIMP-3 plasmid injected into muscle (TIMP-3 IM group), TIMP-3 plasmid electrotransfered into muscle (TIMP-3 ETM group), TIMP-3 plasmid electrotransfered into implanted tumor (TIMP-3 ETT group). Electrical parameters used in electrotransfer were: 200 V/cm, 20 ms/pulse; 8 pulses, 1 Hz in muscle and 600 V/cm, 20 ms/pulse; 1 pulses, 1 Hz in implanted tumor respectively.
ResultsThe growth of TA-K was inhibited more significantly in the groups of TIMP-3 plasmid electrotransfered into muscle and TIMP-3 plasmid electrotransfered into implanted tumor than the other groups (P＜0.05), and the content of TIMP-3 protein in tumor tissues was higher in these two groups .
ConclusionAnti-oncogene has the antitumor effects by DNA electrotransfer in muscle.

Citation： SUN Hui,WANG Jinguo,FU Yantao,LUAN Shan,ZHENG Zelin.. Effects of DNA Electrotransfer in Muscle on the Implanted Tumor Growth in Nude Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(1): 51-54. doi: Copy

1.	Goto T, Nishi T, Tamura T, et al. Highly efficient electrogene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene [Ｊ]. Proc Natl Acad Sci USA, 2000; 97(1)∶354.
2.	Folkman J. The role of angiogenesis in tumor growth [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶65.
3.	Bicknell R, Harris AL. Mechanisms and therapeutic implications of angiogenesis [Ｊ]. Curr Opin Oncol, 1996; 8(1)∶60.
4.	Pluda JM. Tumorassociated angiogenesis: mechanisms, clinical implications, and therapeutic strategies [Ｊ]. Semin Oncol, 1997; 24(2)∶203.
5.	Folkman J, Ingber D. Inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶89.
6.	Bicknell R, Harris AL. Anticancer strategies involving the vasculature: vascular targeting and the inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(6)∶399.
7.	Scott PA, Harris AL. Current approaches to targeting cancer using antiangiogenesis therapies [Ｊ]. Cancer Treat Rev, 1994; 20(4)∶393.
8.	Curran S, Murray GI. Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis [Ｊ]. Eur J Cancer, 2000; 36(13)∶1621.
9.	Baker AH, George SJ, Zaltsman AB, et al. Inhibition of invasion and induction of apoptotic cell death of cancer cell lines by overexpression of TIMP3 [Ｊ]. Br J Cancer, 1999; 79(9-10)∶1347.
10.	Ahonen M, Baker AH, Kahari VM. Adenovirusmediated gene delivery of tissue inhibitor of metalloproteinases3 inhibits invasion and induces apoptosis in melanoma cells [Ｊ]. Cancer Res, 1998; 58(11)∶2310.
11.	AnandApte B, Bao L, Smith R, et al. A review of tissue inhibitor of metalloproteinases3 (TIMP3) and experimental analysis of its effect on primary tumor growth [Ｊ]. Biochem Cell Biol, 1996; 74(6)∶853.
12.	Bian J, Wang Y, Smith MR, et al. Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)3 [Ｊ]. Carcinogenesis , 1996; 17(9)∶1805.
13.	Spurbeck WW, Ng CY, Strom TS, et al. Enforced expression of tissue inhibitor of matrix metalloproteinase3 affects functional capillary morphogenesis and inhibits tumor growth in a murine tumor model [Ｊ]. Blood, 2002; 100(9)∶3361.
14.	Smith LC, Nordstrom JL. Advances in plasmid gene delivery and expression in skeletal muscle [Ｊ]. Curr Opin Mol Ther, 2000; 2(2)∶150.
15.	Muramatsu T, Nakamura A, Park HM. In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals (Review) [Ｊ]. Int J Mol Med, 1998; 1(1)∶55.
16.	Somiari S, GlasspoolMalone J, Drabick JJ, et al. Theory and in vivo application of electroporative gene delivery [Ｊ]. Mol Ther, 2000; 2(3)∶178.
17.	Vitadello M, Schiaffino MV, Picard A, et al. Gene transfer in regenerating muscle [Ｊ]. Hum Gene Ther, 1994; 5(1)∶11.

1. Goto T, Nishi T, Tamura T, et al. Highly efficient electrogene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene [Ｊ]. Proc Natl Acad Sci USA, 2000; 97(1)∶354.
2. Folkman J. The role of angiogenesis in tumor growth [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶65.
3. Bicknell R, Harris AL. Mechanisms and therapeutic implications of angiogenesis [Ｊ]. Curr Opin Oncol, 1996; 8(1)∶60.
4. Pluda JM. Tumorassociated angiogenesis: mechanisms, clinical implications, and therapeutic strategies [Ｊ]. Semin Oncol, 1997; 24(2)∶203.
5. Folkman J, Ingber D. Inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶89.
6. Bicknell R, Harris AL. Anticancer strategies involving the vasculature: vascular targeting and the inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(6)∶399.
7. Scott PA, Harris AL. Current approaches to targeting cancer using antiangiogenesis therapies [Ｊ]. Cancer Treat Rev, 1994; 20(4)∶393.
8. Curran S, Murray GI. Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis [Ｊ]. Eur J Cancer, 2000; 36(13)∶1621.
9. Baker AH, George SJ, Zaltsman AB, et al. Inhibition of invasion and induction of apoptotic cell death of cancer cell lines by overexpression of TIMP3 [Ｊ]. Br J Cancer, 1999; 79(9-10)∶1347.
10. Ahonen M, Baker AH, Kahari VM. Adenovirusmediated gene delivery of tissue inhibitor of metalloproteinases3 inhibits invasion and induces apoptosis in melanoma cells [Ｊ]. Cancer Res, 1998; 58(11)∶2310.
11. AnandApte B, Bao L, Smith R, et al. A review of tissue inhibitor of metalloproteinases3 (TIMP3) and experimental analysis of its effect on primary tumor growth [Ｊ]. Biochem Cell Biol, 1996; 74(6)∶853.
12. Bian J, Wang Y, Smith MR, et al. Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)3 [Ｊ]. Carcinogenesis , 1996; 17(9)∶1805.
13. Spurbeck WW, Ng CY, Strom TS, et al. Enforced expression of tissue inhibitor of matrix metalloproteinase3 affects functional capillary morphogenesis and inhibits tumor growth in a murine tumor model [Ｊ]. Blood, 2002; 100(9)∶3361.
14. Smith LC, Nordstrom JL. Advances in plasmid gene delivery and expression in skeletal muscle [Ｊ]. Curr Opin Mol Ther, 2000; 2(2)∶150.
15. Muramatsu T, Nakamura A, Park HM. In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals (Review) [Ｊ]. Int J Mol Med, 1998; 1(1)∶55.
16. Somiari S, GlasspoolMalone J, Drabick JJ, et al. Theory and in vivo application of electroporative gene delivery [Ｊ]. Mol Ther, 2000; 2(3)∶178.
17. Vitadello M, Schiaffino MV, Picard A, et al. Gene transfer in regenerating muscle [Ｊ]. Hum Gene Ther, 1994; 5(1)∶11.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of DNA Electrotransfer in Muscle on the Implanted Tumor Growth in Nude Mice

Abstract Full text Figures/Tables Video References Cited by

Format

Content