Evaluation of Sclerosing Effects of Several Chemical Sclerosants on Gallbladder Mucosa_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XU Zhi , PENG Xuanfu , WANG Lixin , ZHANG Nengwei , DENG Shaoqing , ZHOU Xiaosi

Department of Surgery, The Third Affiliated Hospital,Beijing University, Beijing 100083, China;

Corresponding author：

Keywords：

Chemical sclerosant; Gallbladder

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Objective To evaluate the sclerosing effects of several chemical sclerosants on the gallbadder mucosa in vivo.
Methods Twentyeight rabbits were randomly divided into： Normal saline was used as contrast agent, and 95% ethanol, 5% tetracycline and compound phenol as sclerosants respectively. The cystic duct was ligated and cholecystostomy was performed for each animal. Sclerosants and contrast agent were injected into the gallbladder through a tube respectively and were kept in the gallbladder for 15 minutes. Animals were killed two weeks later; the gallbladder, bile duct and adjacent liver tissue were obtained for histopathological examination.
Results With the naked eye, normal saline and 5% tetracycline made the cavity of the gallbladder unchanged; 95% ethanol made two gallbladder cavities occluded, two smaller, and three unchanged; and compound phenol made four gallbladder cavities occluded, and three smaller. In histopathological observations, 95% ethanol and compound phenol could make seven rabbits’ gallbladder mucosa completely destroyed (7/7); compared with normal saline (1/7), there was significant difference (P＜0.01). Five percent tetracycline had some effect of ablating the gallbladder mucosa (5/7), but compared with normal saline, there was no difference (P gt;0.05).
Conclusion Ninety-five percent ethanol can be clinically applied as an effective and safe sclerosant.

Citation： XU Zhi,PENG Xuanfu,WANG Lixin,ZHANG Nengwei,DENG Shaoqing,ZHOU Xiaosi. Evaluation of Sclerosing Effects of Several Chemical Sclerosants on Gallbladder Mucosa. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 136-138. doi: Copy

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7.	Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [Ｊ]. Radiology, 1990; 176(3)∶687.
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1. Majeed AW, Reed MW, Stephenson TJ, et al. Chemical ablation of the gallbladder [Ｊ]. Br J Surg, 1997; 84(5)∶638.
2. Soulen MC, Sokol MC, Sullivan KL. Chemical ablation of the gallbladder: evaluation of multiple agents in vitro [Ｊ]. J Vasc Interv Radiol, 1994; 5(5)∶765.
3. Uchiyama N, Stridbeck H, Stenram U. Chemical sclerosis of the gallbladder. An experimental study in pigs of the effect of absolute ethanol and polidocanol on gallbladder epithelium [Ｊ]. Acta Radiol, 1989; 30(4)∶427.
4. Lindberg CG, Lundstedt C, Jeppsson B, et al. Sclerotherapy for ablation of the gallbladder after gallstone lithotripsy with a mechanical lithotriptor. An experimental study in pigs on the effect of absolute ethanol on edematous gallbladder wall [Ｊ]. Acta Radiol, 1991; 32(6)∶521.
5. Soulen MC, Sullivan KL. Chemical ablation of the gallbladder: is it feasible ? [Ｊ]. J Vasc Interv Radiol, 1995; 6(4)∶553.
6. Becker CD, Quenville NF, Burhenne HJ. Gallbladder ablation through radiologic intervention: an experimental alternative to cholecystectomy [Ｊ]. Radiology, 1989; 171(1)∶235.
7. Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [Ｊ]. Radiology, 1990; 176(3)∶687.
8. Ji ZL, Li H, Chen HR, et al. Endoscopic chemical ablation of the gallbladder [Ｊ]. Br J Surg, 1994; 81(8)∶1193.
9. Getrajdman GI, O’Toole K, Laffey KJ, et al. Cystic duct occlusion and transcatheter sclerosis of the gallbladder in the rabbit [Ｊ]. Invest Radiol, 1986; 21(5)∶400.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Evaluation of Sclerosing Effects of Several Chemical Sclerosants on Gallbladder Mucosa

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