Objective To evaluate the sclerosing effects of several chemical sclerosants on the gallbadder mucosa in vivo.
Methods Twentyeight rabbits were randomly divided into: Normal saline was used as contrast agent, and 95% ethanol, 5% tetracycline and compound phenol as sclerosants respectively. The cystic duct was ligated and cholecystostomy was performed for each animal. Sclerosants and contrast agent were injected into the gallbladder through a tube respectively and were kept in the gallbladder for 15 minutes. Animals were killed two weeks later; the gallbladder, bile duct and adjacent liver tissue were obtained for histopathological examination.
Results With the naked eye, normal saline and 5% tetracycline made the cavity of the gallbladder unchanged; 95% ethanol made two gallbladder cavities occluded, two smaller, and three unchanged; and compound phenol made four gallbladder cavities occluded, and three smaller. In histopathological observations, 95% ethanol and compound phenol could make seven rabbits’ gallbladder mucosa completely destroyed (7/7); compared with normal saline (1/7), there was significant difference (P<0.01). Five percent tetracycline had some effect of ablating the gallbladder mucosa (5/7), but compared with normal saline, there was no difference (P gt;0.05).
Conclusion Ninety-five percent ethanol can be clinically applied as an effective and safe sclerosant.
Citation:
XU Zhi,PENG Xuanfu,WANG Lixin,ZHANG Nengwei,DENG Shaoqing,ZHOU Xiaosi. Evaluation of Sclerosing Effects of Several Chemical Sclerosants on Gallbladder Mucosa. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 136-138. doi:
Copy
Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
1. |
Majeed AW, Reed MW, Stephenson TJ, et al. Chemical ablation of the gallbladder [J]. Br J Surg, 1997; 84(5)∶638.
|
2. |
Soulen MC, Sokol MC, Sullivan KL. Chemical ablation of the gallbladder: evaluation of multiple agents in vitro [J]. J Vasc Interv Radiol, 1994; 5(5)∶765.
|
3. |
Uchiyama N, Stridbeck H, Stenram U. Chemical sclerosis of the gallbladder. An experimental study in pigs of the effect of absolute ethanol and polidocanol on gallbladder epithelium [J]. Acta Radiol, 1989; 30(4)∶427.
|
4. |
Lindberg CG, Lundstedt C, Jeppsson B, et al. Sclerotherapy for ablation of the gallbladder after gallstone lithotripsy with a mechanical lithotriptor. An experimental study in pigs on the effect of absolute ethanol on edematous gallbladder wall [J]. Acta Radiol, 1991; 32(6)∶521.
|
5. |
Soulen MC, Sullivan KL. Chemical ablation of the gallbladder: is it feasible ? [J]. J Vasc Interv Radiol, 1995; 6(4)∶553.
|
6. |
Becker CD, Quenville NF, Burhenne HJ. Gallbladder ablation through radiologic intervention: an experimental alternative to cholecystectomy [J]. Radiology, 1989; 171(1)∶235.
|
7. |
Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [J]. Radiology, 1990; 176(3)∶687.
|
8. |
Ji ZL, Li H, Chen HR, et al. Endoscopic chemical ablation of the gallbladder [J]. Br J Surg, 1994; 81(8)∶1193.
|
9. |
Getrajdman GI, O’Toole K, Laffey KJ, et al. Cystic duct occlusion and transcatheter sclerosis of the gallbladder in the rabbit [J]. Invest Radiol, 1986; 21(5)∶400.
|
- 1. Majeed AW, Reed MW, Stephenson TJ, et al. Chemical ablation of the gallbladder [J]. Br J Surg, 1997; 84(5)∶638.
- 2. Soulen MC, Sokol MC, Sullivan KL. Chemical ablation of the gallbladder: evaluation of multiple agents in vitro [J]. J Vasc Interv Radiol, 1994; 5(5)∶765.
- 3. Uchiyama N, Stridbeck H, Stenram U. Chemical sclerosis of the gallbladder. An experimental study in pigs of the effect of absolute ethanol and polidocanol on gallbladder epithelium [J]. Acta Radiol, 1989; 30(4)∶427.
- 4. Lindberg CG, Lundstedt C, Jeppsson B, et al. Sclerotherapy for ablation of the gallbladder after gallstone lithotripsy with a mechanical lithotriptor. An experimental study in pigs on the effect of absolute ethanol on edematous gallbladder wall [J]. Acta Radiol, 1991; 32(6)∶521.
- 5. Soulen MC, Sullivan KL. Chemical ablation of the gallbladder: is it feasible ? [J]. J Vasc Interv Radiol, 1995; 6(4)∶553.
- 6. Becker CD, Quenville NF, Burhenne HJ. Gallbladder ablation through radiologic intervention: an experimental alternative to cholecystectomy [J]. Radiology, 1989; 171(1)∶235.
- 7. Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [J]. Radiology, 1990; 176(3)∶687.
- 8. Ji ZL, Li H, Chen HR, et al. Endoscopic chemical ablation of the gallbladder [J]. Br J Surg, 1994; 81(8)∶1193.
- 9. Getrajdman GI, O’Toole K, Laffey KJ, et al. Cystic duct occlusion and transcatheter sclerosis of the gallbladder in the rabbit [J]. Invest Radiol, 1986; 21(5)∶400.