Effects of Bile from Patients with Cholecystolithiasis on the Growth of Human Gallbladder Carcinoma Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WU Gaosong , ZOU Shengquan

Department of General Surgery， Tongji Hospital， Tongji Medical College， Huazhong University of Science and Technology， Wuhan 430030， China;

Corresponding author：

Keywords：

Cholecystolithiasis; Bile; Gallbladder carcinoma; Cell proliferation

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Objective To explore the effects of bile from patients with cholecystolithiasis on the growth of human gallbladder carcinoma cells GBC-SD and the potential correlation between cholecystolithiasis and gallbladder carcinoma.
Methods Cholecystolithiasis bile (CB) and normal bile (NB) specimens were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTT) assay and cell cycle and apoptosis were analyzed by flow cytometry.
Results CB can significantly promote the proliferation of GBC-SD cells, GBC-SD proliferative index increased significantly after treated with 1% CB for 48 h （P＜0.05）.The Sphase fraction of CB 〔（49.26±8.07）%〕 increased remarkably (P＜0.05) compared with that of NB 〔（25.54±6.57）%〕, and the CB percentage of G0/G1 phase 〔（40.59±9.12）%〕 decreased remarkably (P＜0.05) compared with NB 〔(60.64±13.42）〕%.
Conclusion CB can promote the proliferation of human gallbladder carcinoma GBC-SD cells.

Citation： WU Gaosong,ZOU Shengquan. Effects of Bile from Patients with Cholecystolithiasis on the Growth of Human Gallbladder Carcinoma Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(2): 159-161. doi: Copy

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1. 刘博，王占民，吴小鹏，等.人胆囊癌细胞系GBCSD的建立 [J].山东医科大学学报， 2000； 38(3)∶332.
2. 石景森.胆囊癌与胆囊结石的相关研究 [J].中华肝胆外科杂志， 2001； 7（2）∶116.
3. 石景森，杨毅军. 原发性胆囊癌与胆囊结石关系研究的新进展 [J].中华普通外科杂志， 2000； 15（6）∶373　.
4. 马浙夫，王忠裕，张俊然.成石胆汁在胆囊癌致病因素中的作用 [J].中华医学杂志， 2001； 81(5)∶268.
5. Wu GS, Zou SQ, Luo XW, et al. Proliferative activity of bile from congenital choledochal cyst patients [J]. World J Gastroenterol， 2003； 9(1)∶184.
6. Wu GS, Zou SQ, Liu ZR, et al. Bile from a patient with anomalous pancreaticobiliary ductal union promotes the proliferation of human cholangiocarcinoma cells via COX2 pathway [J].World J Gastroenterol， 2003； 9(5)∶1094.
7. Sugiyama Y, Kobori H, Hakamada K, et al. Altered bile composition in the gallbladder and common bile duct of patients with anomalous pancreaticobiliary ductal junction [J].World J Surg, 2000； 24(1)∶17.
8. Chijiiwa K, Nagai E, Makino I, et al. Are secondary bile acids in choledochal cysts important as a risk factor in biliary tract carcinoma? [J]. Aust N Z J Surg, 1993； 63(2)∶109.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Bile from Patients with Cholecystolithiasis on the Growth of Human Gallbladder Carcinoma Cells

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