Expression of Somatostatin Receptor Ⅱ mRNA in Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Xiaoyi ¹ , TANG Weisong ¹ , CHEN Yuanjia ² , CHEN Yuanfang. ²

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China;

Corresponding author：

Keywords：

Somatostatin receptor; Colorectal neoplasm

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ObjectiveTo detect the expression of somatostatin receptor Ⅱ(SSTR2) mRNA in the specimens of colorectal cancer patients and discuss the relationship between the expression and characteristics of the tumor.MethodsAll tissue samples of 36 cases, including normal colonic mucosa (10 cm beyond the tumor), mucosa adjacent to the tumor (within 2 cm of the tumor), tumor, and mesenteric lymph node, were collected immediately after surgical resection. The SSTR2 mRNA were detected by RT-PCR in 135 samples of the 36 cases. The SSTR2 mRNA expression rate in different samples was compared.ResultsThe SSTR2 mRNA expression rate of normal colonic mucosa, mucosa adjacent to tumor, tumor, mesenteric node without metastasis, and mesenteric node with metastasis was 67.6%(23/34), 75.0%(24/32),91.4%(32/35),93.8%(15/16) and 81.8%(9/11) respectively. Expression rate of tumor significantly increased compared with normal colonic mucosa (χ2=6.37, P＜0.05). The expression rate of the tumor in different groups, such as patients of different sex, serum CEA level, tumor size, location, histological grade and pathological stage, showed no difference (P＞0.05）.ConclusionThe expression rate of SSTR2 mRNA of colorectal adenocarcinoma increases. The expression rate does not correlate with the histological grade and pathological stage of the tumor.

Citation： LI Xiaoyi,TANG Weisong,CHEN Yuanjia,CHEN Yuanfang.. Expression of Somatostatin Receptor Ⅱ mRNA in Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 347-350. doi: Copy

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1. 陈元方，张万岱. 胃肠肽类激素，生长因子与消化系统非内分泌性恶性肿瘤. 见：陈元方主编. 胃肠肽类激素基础与临床 [M]. 第1版. 北京：北京医科大学中国协和医科大学联合出版社， 1997∶752～760.
2. Pages P, Benali N, SaintLaurent N, et al. sst2 somatostatin receptor mediates cell cycle arrest and induction of p27(Kip1). Evidence for the role of SHP1 [Ｊ]. J Biol Chem, 1999; 274(21)∶15186.
3. Kubota A, Yamada Y, Kagimoto S, et al. Identification of somatostatin receptor subtypes and an implication for the efficacy of somatostatin analogue SMS 201995 in treatment of human endocrine tumors [Ｊ]. J Clin Invest, 1994; 93(3)∶1321.
4. NakajimaIijima S, Hamada H, Reddy P, et al. Molecular structure of the human cytoplasmic betaactin gene: interspecies homology of sequences in the introns [Ｊ]. Proc Natl Acad Sci USA, 1985; 82(18)∶6133.
5. Buscail L, SaintLaurent N, Chastre E, et al. Loss of sst2 somatostatin receptor gene expression in human pancreatic and colorectal cancer [Ｊ]. Cancer Res, 1996; 56(8)∶1823.
6. Laws SA, Gough AC, Evans AA, et al. Somatostatin receptor subtype mRNA expression in human colorectal cancer and normal colonic mucosae [Ｊ]. Br J Cancer, 1997; 75(3)∶360.
7. Vuaroqueaux V, Dutour A, Briard N, et al. No loss of sst receptors gene expression in advanced stages of colorectal cancer [Ｊ]. Eur J Endocrinol, 1999; 140(4)∶362.
8. Vuaroqueaux V, Dutour A, Bourhim N, et al. Increased expression of the mRNA encoding the somatostatin receptor subtype five in human colorectal adenocarcinoma [Ｊ]. J Mol Endocrinol, 2000; 24(3)∶397.
9. Reubi JC, Waser B, Schmassmann A, et al. Receptor autoradiographic evaluation of cholecystokinin, neurotensin, somatostatin and vasoactive intestinal peptide receptors in gastrointestinal adenocarcinoma samples: where are they really located? [Ｊ]. Int J Cancer, 1999; 81(3)∶376.
10. Stolz B, Weckbecker G, SmithJones PM, et al. The somatostatin receptortargeted radiotherapeutic 90YDOTADPhe1, Tyr3 octreotide (90YSMT 487) eradicates experimental rat pancreatic CA 20948 tumours [Ｊ]. Eur J Nucl Med, 1998; 25(7)∶668.
11. Plonowski A, Schally AV, Nagy A, et al. Inhibition of metastatic renal cell carcinomas expressing somatostatin receptors by a targeted cytotoxic analogue of somatostatin AN238 [Ｊ]. Cancer Res, 2000; 60(11)∶2996.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Somatostatin Receptor Ⅱ mRNA in Colorectal Cancer

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