Expression of Toll-Like Receptor 2 in Liver Kupffer Cell and Sinsusoidal Endothelial Cell in Hepatic Endogenous Injury in Mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HUANG Wenguang ¹ , WANG Feng ¹ , LI Jie ¹ , WU Heshui ¹ , WANG Lin ² , ZHANG Jinxiang ¹ , TIAN Yuan ¹ , ZHANG Jinhui. ¹

Department of General Surgery, Tongji Medical College, University of Huazhong Science and Technology, Wuhan 430022, China;

Corresponding author：

Keywords：

Ischemia-reperfusion injury Kupffer cell Toll-like receptor

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【Abstract】Objective To observe the synthesis of TLR2 protein and its mRNA expression in Kupffer cells (KCs) and sinusoidal endothelial cells(SECs).
Methods Thirty-two BALB/c mice divided into two groups (operation group and false operation group) were used to prepare the model of partial hepatic ischemia/reperfusion (I/R) injury. After injury KCs and SECs were isolated with twosteps situ perfusion technique. And these cells were dyed by rat anti-mouse TLR2 IgG and anti-rat IgG2b labeled with flurescein isothiocyanate (FITC). The sysnthesis of TLR2 protein were determined by flow cytometric (FCM) analysis and real time reverse transcription polymerase chain reaction (Real-Time RT-PCR) analysis for gene expression.
Results As for KCs: TLR2 expression was significant higher in operation group, compared with false operation group 〔protein expression: （9.19±1.07）% vs （1.52±0.21）%, P＜0.01; gene expression: 0.54±0.77 vs 2.62±2.19, P＜0.05〕. But there were no significant differences with expression in SECs.
Conclusion Synthesis of TLR2 protein and its gene expression increased in KCs in the mouse partial hepatic ischemia-reperfusion injury.

Citation： HUANG Wenguang,WANG Feng,LI Jie,WU Heshui,WANG Lin,ZHANG Jinxiang,TIAN Yuan,ZHANG Jinhui.. Expression of Toll-Like Receptor 2 in Liver Kupffer Cell and Sinsusoidal Endothelial Cell in Hepatic Endogenous Injury in Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 385-388. doi: Copy

1.	Vasselon T, Detmers PA. Toll receptors: a central element in innate immune responses [Ｊ]. Infect Immun， 2002; 70(3)∶ 1033.
2.	Paterson HM, Murphy TJ, Purcell EJ, et al. Injury primes the innate immune system for enhanced Tolllike receptor reactivity [Ｊ]. J Immunol, 2003; 171(3)∶1473.
3.	吴河水，王琳，田元，等. Toll样受体参与小鼠肝脏缺血再灌注损伤 [Ｊ]. 中华肝脏病杂志， 2003； 11（7）∶424.
4.	Braet F, De Zanger R, Sasaoki T, et al. Assessment of a method of isolation, purification, and cultivation of rat liver sinusoidal endothelial cells [Ｊ]. Lab Invest, 1994; 70(6)∶944.
5.	Dabbagh K, Lewis DB. Tolllike receptors and Thelper1/Thelper2 responses [Ｊ]. Curr Opin Infect Dis, 2003; 16(3)∶199.
6.	Kaisho T, Akira S. Regulation of dendritic cell function through Tolllike receptors [Ｊ]. Curr Mol Med, 2003; 3(4)∶373.
7.	王英杰主编. 生物人工肝 [M]. 第1版. 北京：人民卫生出版社， 2002∶119～160.

1. Vasselon T, Detmers PA. Toll receptors: a central element in innate immune responses [Ｊ]. Infect Immun， 2002; 70(3)∶ 1033.
2. Paterson HM, Murphy TJ, Purcell EJ, et al. Injury primes the innate immune system for enhanced Tolllike receptor reactivity [Ｊ]. J Immunol, 2003; 171(3)∶1473.
3. 吴河水，王琳，田元，等. Toll样受体参与小鼠肝脏缺血再灌注损伤 [Ｊ]. 中华肝脏病杂志， 2003； 11（7）∶424.
4. Braet F, De Zanger R, Sasaoki T, et al. Assessment of a method of isolation, purification, and cultivation of rat liver sinusoidal endothelial cells [Ｊ]. Lab Invest, 1994; 70(6)∶944.
5. Dabbagh K, Lewis DB. Tolllike receptors and Thelper1/Thelper2 responses [Ｊ]. Curr Opin Infect Dis, 2003; 16(3)∶199.
6. Kaisho T, Akira S. Regulation of dendritic cell function through Tolllike receptors [Ｊ]. Curr Mol Med, 2003; 3(4)∶373.
7. 王英杰主编. 生物人工肝 [M]. 第1版. 北京：人民卫生出版社， 2002∶119～160.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Toll-Like Receptor 2 in Liver Kupffer Cell and Sinsusoidal Endothelial Cell in Hepatic Endogenous Injury in Mice

Abstract Full text Figures/Tables Video References Cited by

Format

Content