【Abstract】Objective To investigate the expressions of TNF-α and superoxide dismutase (SOD) mRNA in myocardium of rats with obstructive jaundice (OJ).
Methods The expressions of TNF-α and SOD mRNA were semi-quantitatively analyzed after amplification of cDNA in myocardium of the rats with OJ by RTPCR.
Results The expression of TNF-α mRNA increased and that of SOD mRNA decreased in the myocardium of rats with OJ. The level of plasm TNF-α increased and SOD synthesis in myocardium decreased.
Conclusion The injury of myocardium in
OJ is correlated with increasing expression of TNF-α mRNA and decreasing expression of SOD mRNA.
Citation：
GONG Peng,WANG Zhongyu,ZHAO Zuowei,WANG Hongjiang,GUAN Fenglin.. Expression of TNF-α and Superoxide Dismutase mRNA in Myocardium of Rats with Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(6): 502-504. doi:
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- 1. O’Neil S, Hunt J, Filkins J, et al. Obstructive jaundice in rats results in exaggerated hepatic production of tumor necrosis factoralpha and systemic and tissue tumor necrosis factoralpha levels after endotoxin [Ｊ]. Surgery, 1997; 122(2)∶ 281.
- 2. Kennedy JA, Lewis H, Clements WD, et al. Kupffer cell blockade, tumour necrosis factor secretion and survival following endotoxin challenge in experimental biliary obstruction [Ｊ]. Br J Surg, 1999; 86(11)∶1410.
- 3. 巩鹏， 王忠裕， 李克军， 等. 胆道梗阻后心肌损伤的实验研究 [Ｊ]. 肝胆胰外科杂志， 2002； 4（3）∶157.
- 4. 巩鹏， 王忠裕，王洪江，等. pLNCXSOD基因转染大鼠心肌细胞对胆汁毒性损害的保护作用 [Ｊ]. 中华实验外科杂志， 2002； 19（增刊）∶155.
