Separation, Purification and Amplification of Dendritic Cells from Peripheral Blood of Patients with Pancreatic Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TANG Zhao hui ¹ , ZOU Sheng quan ¹ , QIU Wen hong ² , YANG Xiang ping ³ , QIU Fa zu ¹

Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030，China;

Corresponding author：

Keywords：

Pancreatic carcinoma; Dendritic cell; Granulocyte/macrophage colony; stimulating factorInterleukin-4

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ObjectiveTo find out an effective method for amplification and purification of dendritic cells(DC) from peripheral blood of patients with pancreatic carcinoma. MethodsPeripheral blood mononuclear cells were purified from peripheral blood of health volunteers(control group,10 cases) and patients with pancreatic carcinoma (experimental group,12 cases) with incubation of granulocyte/macrophage colonystimulating factor(GMCSF) and interleukin4(IL4).The quality of DC were detected by immumofluorescence method and the expression levels of HLADR and B72 on DC were detected by flow cytometer after and before DC incubation with GMCSF and the IL4. ResultsThe expression level of HLADR and B72 of DC in experimental group were significantly less than those in control group(P＜0.01).DC in experimental group was significantly proliferated in the presence of GMCSF and IL4(P＜0.01).On day 7,the expression level of HLADR and B72 of DC in experimental group were significantly increased(P＜0.01) and there was no difference versus control group(P gt;0.05).ConclusionIt is suggested that combination of GMCSF and IL4 can selectively and effectively enhance proliferation and immune function of DC from peripheral blood of patient with pancreatic carcinoma.

Citation： TANG Zhao hui,ZOU Sheng quan,QIU Wen hong,YANG Xiang ping,QIU Fa zu. Separation, Purification and Amplification of Dendritic Cells from Peripheral Blood of Patients with Pancreatic Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(1): 20-21. doi: Copy

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Separation, Purification and Amplification of Dendritic Cells from Peripheral Blood of Patients with Pancreatic Carcinoma

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