The Effects of Somatostatin Analog on the Apoptosis and the Expression of cmyc Protein of Hepatocellular Carcinoma Cell_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Yanling , ZHANG Zheng

Department of Gerontism, West China Hospital, Sichuan University, Chengdu 610041， China;

Corresponding author：

Keywords：

Somatostatin analog; Hepatocellular carcinoma SMMC-7721; Apoptosis; c-myc protein expression

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ObjectiveTo investigate the effects of somatostatin8 (SS8) on the apoptosis and the expression of cmyc protein of hepatocellular carcinoma cell SMMC7721. MethodsCultured in vitro, hepatocellular carcinoma cells SMMC7721 were incubated with SS8 (10 μg/ml). The apoptosis rate and expression of cmyc protein were detected by flow cytometry (FCM). ResultsSS8 can cause the spanonumber in S and G2/M phase and the auxonumber in G0/G1 phase of SMMC7721 cells . The apoptosis rate was 14.2% in the study group and 6.1% in the control group, and there was significant difference (P＜0.05); The level of expressions of cmyc protein was 0.833±0.035 after action by SS8 for 24 h. Compared with control group, there was no significant difference in the study group（P＞0.10）.But after the cells were incubated with SS8 for 48，72，96，120，144 h, the level of expressions of cmyc protein was 0.818±0.04，0.721±0.029，0.669±0.026，0.648±0.045，0.642±0.028 respectively in the study group, and there was significant difference as compared with the control group (P＜0.05). Conclusion The SS8 can induce the apoptosis and lower expression of cmyc protein of hepatocellular carcinoma cell SMMC7721.

Citation： ZHANG Yanling,ZHANG Zheng. The Effects of Somatostatin Analog on the Apoptosis and the Expression of cmyc Protein of Hepatocellular Carcinoma Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(1): 25-27. doi: Copy

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1. Robbins RJ. Somatostatin and cancer [Ｊ]. Metabolism，1996; 45(8 Suppl 1)∶98.
2. Srikant CB.Cell cycle dependent induction of apoptosis by somatostatin analog SMS201995 in AtT20 mouse pituitary cells [Ｊ]. Biochem Biophys Res Commun，1995; 209(2)∶400.
3. Reubi JC. Clinical relevance of somatostatin receptor imaging [Ｊ]. Eur J Endocrinol，1994; 131(6)∶575.
4. Reubi JC, Laissue JA. Multiple actions of somatostatin in neoplastic disease [Ｊ]. Trends Pharmacol Sci，1995; 16(3)∶110.
5. Sharma K, Patel YC, Srikant CB. Subtypeselective induction of wildtype p53 and apoptosis, but not cell cycle arrest, by human somatostatin receptor 3 [Ｊ]. Mol Endocrinol，1996; 10(12)∶1688.
6. Patel YC, Panetta R, Escher E,et al. Expression of multiple somatostatin receptor genes in AtT20 cells. Evidence for a novel somatostatin28 selective receptor subtype [Ｊ]. J Biol Chem， 1994; 269(2)∶1506.
7. Weinstein IB. The origins of human cancer: molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatmenttwentyseventh G.H.A. Clowes memorial award lecture [Ｊ]. Cancer Res，1988; 48(15)∶4135.
8. Stanbridge EJ. The reemergence of tumor suppression [Ｊ]. Cancer Cells, 1989; 1(1)∶31.
9. Lebovitz RM, Lieberman MW. Modulation of cellular genes by oncogenes [Ｊ]. Prog Nucleic Acid Res Mol Biol, 1988; 35∶73.
10. Pinto A, Attadia V, Rosati R,et al.Differentiation of human leukaemic cell lines and fresh leukaemia cells by low dose AraC: monitoring by expression of cmyc and cfos oncogenes [Ｊ]. Med Oncol Tumor Pharmacother, 1988; 5(2)∶91.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Effects of Somatostatin Analog on the Apoptosis and the Expression of cmyc Protein of Hepatocellular Carcinoma Cell

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