Expression of p27 in Hepatocellular Carcinoma and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHOU Qi ¹ , WANG Guangtian ² , LIANG Lijian ¹ , ZHENG Wei ² , PANG Zhigang ²

Department of General Surgery, The First Affiliated Hospital of Sun Yatsen University of Medical Sciences, Guangzhou 510080,China;

Corresponding author：

Keywords：

Hepatocellular carcinoma; p27 protein; Metastasis

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ObjectiveTo study the expression of p27 in hepatocellular carcinoma (HCC) and its clinical significance.MethodsFortyfive specimens of HCC and 30 specimens of adjacent noncancerous lesions obtained from 45 patients who underwent surgery were examined for p27 expression by immunohistochemistry SABC method. The diameter of tumor ranged from 1 cm to 19 cm (d ≤5 cm, 9 samples; 5 cm lt;d≤10 cm, 19 samples; d＞10 cm, 17 samples). These tumors were graded according to the criteria described by EdmondsonSteiner: highdifferentiated HCC group (Grade Ⅰ＋Ⅱ), 26 samples; lowdifferentiated HCC group (Grade Ⅲ＋Ⅳ), 19 samples. According to the clinicopathological features: 19 samples were poorly encapsulated, 15 samples had portal invasion, 11 samples had extrahepatic metastasis, 12 samples had intrahepatic metastasis; all of the above were classified as the invasive and metastatic group, while the others were classified as the noninvasive and nonmetastatic group. ResultsThe average labeling index (LI) of p27 in HCC was significantly higher than that of adjacent noncancerous lesions (45.87±14.21 vs 33.77±12.92, P lt;0.001). The LI of p27 in lowdifferentiated HCC group (stage Ⅲ＋Ⅳ) was significantly lower than that of highdifferentiated group (stage Ⅰ＋Ⅱ), 34.46±12.29 vs 52.80±11.36 (P lt;0.001). The LI of p27 had significant difference between the large ones (d＞10 cm, 37.59±13.12) and the small ones (d≤5 cm, 53.28±15.17 or 5 cm lt;d≤10 cm, 49.50±10.96) (P lt;0.05). The LI of p27 in the invasive and metastatic group was significantly lower than that in the noninvasive and nonmetastatic group (41.42±12.86 vs 51.44±14.10, P lt;0.05).ConclusionThe expression of p27 is more frequently detected in HCC than in adjacent noncancerous lesions. It indicates that p27 might be a compensatory factor during HCC carcinogenesis. The LI of p27 significantly decreases in poor differentiation group, invasive and metastatic group. It indicates that p27 might be related with the differentiation, invasion and metastasis of HCC.

Citation： ZHOU Qi,WANG Guangtian,LIANG Lijian,ZHENG Wei,PANG Zhigang. Expression of p27 in Hepatocellular Carcinoma and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(2): 134-136. doi: Copy

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1. 夏加增，杨继昌，张熔熔，等.胃癌中Cyclin D1、 Cyclin E和p27的表达及其意义 [J]. 中国普外基础与临床杂志，2001； 8（3）∶148.
2. 郭贵龙，姚榛祥.p27在甲状腺滤泡状肿瘤中的表达及其意义 [J]. 中国普外基础与临床杂志，2001； 8（5）∶324.
3. 姚旻，周信达，刘银坤，等. 上皮钙粘蛋白（Ecadherin）在高侵袭性肝细胞癌中的表达 [J]. 中华消化杂志，1998； 18（1）∶31.
4. Ito Y, Matsuura N, Sakon M, et al. Expression and prognostic roles of the G1S Modulators in hepatocellular carcinoma: p27 independently predicts the recurrence [J]. Hepatology，1999； 30(1)∶90.
5. Sgambato A, Cittadini A, Faraglia B, et al. Multiple functions of p27kip1 and its alterations in tumor cells: A review [J]. J Cell Physio，2000； 183(1)∶18.
6. Pagano M, Tam SW, Theodoras AM, et al. Role of the ubiquitin proteasome pathway in regulating abundance of the cyclindependent kinase inhibitor p27 [J]. Science，1995； 269(5224)∶682.
7. Ferrando AA, Balbin M, Pendas AM, et al. Mutational analysis of the human cyclin dependent kinase inhibitor p27kip1 in primary breast carcinomas [J]. Hum Genet， 1996； 97(1)∶91.
8. Hengst L, Reed SI. Translational control of p27kip1accumulation during the cell cycle [J]. Science，1996； 271(5257)∶1861.
9. Yamamoto H, Soh JW, Shirin H, et al. Comparative effects of overexpression of p27kip1 and p21cip/Waf1 on growth and differentiation in human colon carcinoma cells [J]. Oncogene， 1999； 18(1)∶103.
10. Levenberg S, Yarden A, Kam Z, et al. p27 is involved in Ncadherinmediated contact inhibition of cell growth and Sphase entry [J]. Oncogene，1999； 18(4)∶869.
11. Fang F, Orend G, Watanabe N, et al. Dependence of cyclin ECDK2 kinase activity on cell anchorage [J]. Science，1996； 271(5248)∶499.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of p27 in Hepatocellular Carcinoma and Its Clinical Significance

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