ObjectiveTo investigate the possible mechanism affecting liver cirrhosis by splenectomy. MethodsBy subcutaneous administration of 20% carbon tetrachloride(CCl4), liver cirrhosis models were established in splenectomy and nonsplenectomy groups. After HE staining, special staining and immunohistochemical staining, mast cell, Kupffer’s cell and Ito cell were counted under optical microscope. Liver pathological sections and the dynamic changes of these cells in mice were studied respectively in comparison with the normal group.ResultsThe incidence of liver cirrhosis in nonsplenectomy group was significantly higher than that in splenectomy group after the 16th injection of CCl4 (P＜0.05). The count of mast cell was much higher than that in splenectomy group after the 4th and the 8th injection (P＜0.05). Kupffer’s cell and Ito cell significantly increased after the 12th and the 16th injection in nonsplenectomy group compared with splenectomy group (P＜0.05). ConclusionSplenectomy may decline the incidence of hepatic cirrhosis caused by multifactors. In the early stage, splenectomy influences the migration, maturation and accumulation of mast cell. In the middle and late stage, it influences the proliferation of Kupper’s cell and cytokine secretion, thus the Ito cells are activated and proliferation is inhibited, in which extracellular matrix decreases in amount and the degree of hepatic fibrosis is reduced.
Citation：
PENG Jianping,YE Decun,GU Xia,LUO Jun,HE Sheng.. Study of Mechanism Affecting Liver Cirrhosis by Splenectomy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 222-225. doi:
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- 4. Farrell DJ，Hines JE，Walls AF，et al．Intrahepatic mast cells in chronic liver diseases [J].Hepatology，1995; 22(4 Pt 1)∶1175.
- 5. 朱永红，胡大荣． 肥大细胞在感染及免疫中的作用研究进展 [J]. 国外医学免疫学分册，1999; 22（2）∶96.
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- 10. 汪谦， 夏穗生， 姜汉英， 等． 脾脏及促吞噬肽在体外肝细胞培养过程的免疫调控作用 [J]. 中华实验外科杂志， 1995; 12(5)∶314.
