The Relationship Between Mutation of p53 Gene, p21 Gene and Bacteria LForm in Gallbladder Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

JIAO Xingyuan ¹ , Huang Jiefu ¹ , SHI Jingsen ²

Department of Hepatobiliary Surgery, The First Affiliated Hospital, Sun Yatsen University, Guangzhou 510080, China;

Corresponding author：

Keywords：

Gallbladder carcinoma; Bacteria Lform; p21 gene; p53 gene

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ObjectiveTo study the relationship between mutation of the p53 gene, p21 gene and bacteria Lform in gallbladder carcinoma. MethodsForty cases of gallbladder carcinoma and 40 cases of chronic cholecystitis were studied by using Gram staining and immunohistochemistry (SP method) to detect the positive rate of Lforms antigen, p21 and p53 protein overpression. And the relationship between the expression of p21 and p53 in Lform infection positive group and that in Lform infection negative group was discussed. ResultsThere was no statistical difference between the Lform positive rate in patients with gallbladder carcinoma with Gram staining and immunohistochemistry (P gt;0.05). The positive expression rate of p21 and p53 in gallbladder carcinoma was 62.5%(25/40) and 65.0%(26/40) respectively. The expression values of p21 and p53 in chronic cholecystitis was 2.5%(1/40) and 5.0%(2/40) respectively, which was significantly different from that of gallbladder carcinoma (P＜0.05). The expression of p21 and p53 was significantly higher in Lform infection positive group than in that with Lform infection negative group (P＜0.05). ConclusionBacteria Lform may be one of the direct factor leading to mutation of p53 and p21 during gallbladder oncogenesis.

Citation： JIAO Xingyuan,Huang Jiefu,SHI Jingsen. The Relationship Between Mutation of p53 Gene, p21 Gene and Bacteria LForm in Gallbladder Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 246-248. doi: Copy

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12.	焦兴元，黄洁夫，吕明德，等. L型细菌感染对胆囊粘膜肿瘤坏死因子和血小板生长因子水平的影响 [Ｊ]. 中国普通外科杂志， 2002； 11（2）∶102.
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14.	陈劲松，熊寿芝，杨竹林. 胆囊癌组织中p16、p53及bcl2基因蛋白的表达及意义 [Ｊ]. 中国普外基础与临床杂志，1999； 6（2）∶83.

1. 焦兴元，吕明徳，黄洁夫，等. 胆囊炎、胆囊结石及其它相关危险因素与胆囊癌关系的研究进展 [J]. 中华普通外科杂志， 2002； 17（2）∶117.
2. 梁力建，韩雨生. 胆囊癌的诊治进展 [Ｊ]. 中国普外基础与临床杂志， 1999； 6（2）∶113.
3. 黄谷良，林特夫编著. 细菌L型与疾病 [M]. 北京：学苑出版社， 1991∶66~87.
4. Dienes L,Bulliviant S.Morphology and reproduction processes of the Lforms of bacteria II.Comparative study of Lforms and mycoplasm with the electron Microsape [Ｊ]. J Bact, 1968； 95(1)∶672.
5. Domingue GJ Sr, Woody HB. Bacterial persistence and expression of disease [Ｊ]. Clin Microbiol Rev, 1997; 10(2)∶320.
6. 何杰，张世馥，姚敏,等. 宫颈癌细胞中金黄色葡萄球菌L型DNA的检测 [Ｊ]. 中华微生物学与免疫学杂志， 1996; 16(3)∶76.
7. Hoischen C, Ihn W, Gura K, et al. Structural characterization of molecular phospholipid species in cytoplasmic membranes of the cell wallless streptomyces hygroscopicus L form by use of electrospray ionization coupled with collisioninduced dissociation mass spectrometry [Ｊ]. J Bacteriol, 1997; 179(10)∶3437.
8. Yabu K. Saltinduced Lforms of Staphylococcus aureus [Ｊ]. Microbiol Immunol, 1995; 39(2)∶425.
9. 焦兴元，石景森，高金松, 等. 胆囊癌患者细胞和体液免疫水平的测定及临床意义 [Ｊ]. 中国普外基础与临床杂志， 1999; 6(4)∶227.
10. Jiao XY, Shi JS, Wang JS, et al. Effects of radical cholecystectomy on nutritional and immune status in patients with gallbladder carcinoma [Ｊ]. World J Gastroenterol, 2000; 6(3)∶445.
11. 焦兴元，黄洁夫，吕明德，等. L型细菌的研究及其与胆囊病变的关系 [Ｊ]. 中国现代普通外科进展杂志， 2001； 4（1）∶71.
12. 焦兴元，黄洁夫，吕明德，等. L型细菌感染对胆囊粘膜肿瘤坏死因子和血小板生长因子水平的影响 [Ｊ]. 中国普通外科杂志， 2002； 11（2）∶102.
13. 张东，冯元，王平，等. 抑癌基因p16和p53在原发性胆囊癌中的表达及其相关性研究 [Ｊ]. 中国普外基础与临床杂志，2000； 7（2）∶85.
14. 陈劲松，熊寿芝，杨竹林. 胆囊癌组织中p16、p53及bcl2基因蛋白的表达及意义 [Ｊ]. 中国普外基础与临床杂志，1999； 6（2）∶83.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Relationship Between Mutation of p53 Gene, p21 Gene and Bacteria LForm in Gallbladder Carcinoma

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