Measurement of Urinary 8Hydroxydeoxyguanosine Level with a HighPressure Liquid Chromatography Eectrochemical Approach_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

PENG Tao ¹ , YAN Lnan ¹ , LIU Zhiming ² , Hiroshi Kasai ³ , PENG Minhao ² , LI Lequn ²

Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

Highpressure liquid chromatographyelectrochemical detector; 8hydroxydeoxyguanosine; Liver cancer

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ObjectiveTo investigate the methodology of a newly developed highpressure liquid chromatography electrochemical system in urinary 8hydroxydeoxyguanosine (8OHdG) quantification. MethodsQuantification of urinary 8OHdG with ESA 5600 Coularray system among 21 children from liver cancer highrisk areas, Fusui country, in contrast with 63 controls from Nanning city, Guangxi province.ResultsThe resolution, sensitivity, linearity, precision and recovery of this approach all fully meet the requirement of routine urinary 8OHdG quantification. The mean 8OHdG level of this population was (5.26±0.41) ng/mg creatinine, higher than previous report 〔(4.62±0.091) ng/mg creatinine〕 by similar method.ConclusionWith cautious design and modification, this method is reliable and accurate in high throughput quantification of urinary 8OHdG.

Citation： PENG Tao,YAN Lnan,LIU Zhiming,Hiroshi Kasai,PENG Minhao,LI Lequn,Kazuyoshi Tamae,QIN Xue,WEI Zhongliang,LIANG Renxiang,LI Jiaquan. Measurement of Urinary 8Hydroxydeoxyguanosine Level with a HighPressure Liquid Chromatography Eectrochemical Approach. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(3): 281-283. doi: Copy

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1. Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994； 344(13)∶721.
2. Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993； 9(7)∶246.
3. Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997； 387(3)∶147.
4. Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001； 92(1)∶9.
5. Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999； 27(5-6)∶647.
6. Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993； 6(4)∶357.
7. Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992； 13(12)∶2241.
8. Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis， 1997∶23-77.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Measurement of Urinary 8Hydroxydeoxyguanosine Level with a HighPressure Liquid Chromatography Eectrochemical Approach

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