A Study on Endothelia Injury in Rat Liver Graft Recipient and the Protective Effect of Prostaglandin E1_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TIAN Gendong ¹ , HE Yanzheng ¹ , ZHANG Yanmin ² , HE Chunshui ¹

Department of Vascular Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China;

Corresponding author：

Keywords：

Liver transplantation，Endothelial cell，Prostaglandin E1

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ObjectiveTo study the early functional change of sinusoid endothelial cell after liver transplantation in rat, and to investigate the endothelia protective effect of prostaglandin E1(PGE1). MethodsRat orthotopic liver transplantation model was performed in “twocuff method”, grouped as follows: group A served as normal rat blank control, group B as operative control with normal donor, group C as experimental control with shock donor, and group D as experimental group with shock donor and PGE1 administration (n=8 in each group). Transplanted groups (referring to recipients without specific definition) were sacrificed 6 h after operation for blood taken to detect serum liver enzymes (ALT, LDH), malondialdehyde (MDA), nitric oxide (NO) and plasm endothelin (ET). Liver tissue was resected at the same time for standard pathologic examination. Comparison of the difference the results was made between groups. ResultsCold preservation time and anhepatic phase were similar in each group, (2±0.5) h and (15±3) min respectively. All survived 6 h after transplantation (8/8) in group B and D with a survival rate of 100%, only 5 survived 6 h after transplantation in group C (5/8) with a survival rate of 62.5%. Comparing with group C, blood ALT, LDH, MDA, ET decreased and NO increased significantly in group D (P lt;0.05). Marked histologic structural damage was observed in group C, while normal light microscope appearance was better preserved in group C and D. ConclusionMarked sinusoid endothelia injury occurs during liver transplantation. Concentration of serum NO and plasm ET well presents its function. PGE1 relieves endothelia injury by improving hemodynamics and stabilizing sinusoid endothelial cell plasma membrane.

Citation： TIAN Gendong,HE Yanzheng,ZHANG Yanmin,HE Chunshui. A Study on Endothelia Injury in Rat Liver Graft Recipient and the Protective Effect of Prostaglandin E1. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 328-331. doi: Copy

1.	1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
2.	3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ]. Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
3.	4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
4.	5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
5.	6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶ 160.
6.	7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
7.	8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
8.	9季德刚，刘丙义，郑泽霖，等．前列腺素E1对大鼠肝脏再灌注损伤的保护作用 [Ｊ].中国普外基础与临床杂志， 2003; 10(1)∶ 36.
9.	10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
10.	11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
11.	12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
12.	严以群，杨甲梅，王轩，等．大鼠减体积肝移植模型的初步研究 [Ｊ].中华器官移植杂志， 1996； 17(2)∶55.

1. 1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
2. 3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ]. Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
3. 4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
4. 5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
5. 6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶ 160.
6. 7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
7. 8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
8. 9季德刚，刘丙义，郑泽霖，等．前列腺素E1对大鼠肝脏再灌注损伤的保护作用 [Ｊ].中国普外基础与临床杂志， 2003; 10(1)∶ 36.
9. 10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
10. 11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
11. 12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
12. 严以群，杨甲梅，王轩，等．大鼠减体积肝移植模型的初步研究 [Ｊ].中华器官移植杂志， 1996； 17(2)∶55.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A Study on Endothelia Injury in Rat Liver Graft Recipient and the Protective Effect of Prostaglandin E1

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