ObjectiveTo study the early functional change of sinusoid endothelial cell after liver transplantation
in rat, and to investigate the endothelia protective effect of prostaglandin E1(PGE1). MethodsRat orthotopic liver
transplantation model was performed in “twocuff method”, grouped as follows: group A served as normal rat blank
control, group B as operative control with normal donor, group C as experimental control with shock donor, and group
D as experimental group with shock donor and PGE1 administration (n=8 in each group). Transplanted groups (referring
to recipients without specific definition) were sacrificed 6 h after operation for blood taken to detect serum liver
enzymes (ALT, LDH), malondialdehyde (MDA), nitric oxide (NO) and plasm endothelin (ET). Liver tissue was resected at
the same time for standard pathologic examination. Comparison of the difference the results was made between groups.
ResultsCold preservation time and anhepatic phase were similar in each group, (2±0.5) h and (15±3) min
respectively. All survived 6 h after transplantation (8/8) in group B and D with a survival rate of 100%, only 5
survived 6 h after transplantation in group C (5/8) with a survival rate of 62.5%. Comparing with group C, blood
ALT, LDH, MDA, ET decreased and NO increased significantly in group D (P lt;0.05). Marked histologic structural damage
was observed in group C, while normal light microscope appearance was better preserved in group C and D.
ConclusionMarked sinusoid endothelia injury occurs during liver transplantation. Concentration of serum NO and plasm
ET well presents its function. PGE1 relieves endothelia injury by improving hemodynamics and stabilizing sinusoid
endothelial cell plasma membrane.
Citation：
TIAN Gendong,HE Yanzheng,ZHANG Yanmin,HE Chunshui. A Study on Endothelia Injury in Rat Liver Graft Recipient and the Protective Effect of Prostaglandin E1. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 328-331. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis
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3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ].
Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
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4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver
microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
|
| 4. |
5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total
hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
|
| 5. |
6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶
160.
|
| 6. |
7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by
prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
|
| 7. |
8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver
transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
|
| 8. |
9季德刚，刘丙义，郑泽霖，等．前列腺素E1对大鼠肝脏再灌注损伤的保护作用 [Ｊ].中国普外基础与临床杂志， 2003; 10(1)∶
36.
|
| 9. |
10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during
liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
|
| 10. |
11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced
hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
|
| 11. |
12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux
and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998;
1391(2)∶117.
|
| 12. |
严以群，杨甲梅，王轩，等．大鼠减体积肝移植模型的初步研究 [Ｊ].中华器官移植杂志， 1996； 17(2)∶55.
|
- 1. 1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis
and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
- 2. 3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ].
Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
- 3. 4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver
microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
- 4. 5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total
hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
- 5. 6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶
160.
- 6. 7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by
prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
- 7. 8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver
transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
- 8. 9季德刚，刘丙义，郑泽霖，等．前列腺素E1对大鼠肝脏再灌注损伤的保护作用 [Ｊ].中国普外基础与临床杂志， 2003; 10(1)∶
36.
- 9. 10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during
liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
- 10. 11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced
hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
- 11. 12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux
and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998;
1391(2)∶117.
- 12. 严以群，杨甲梅，王轩，等．大鼠减体积肝移植模型的初步研究 [Ｊ].中华器官移植杂志， 1996； 17(2)∶55.
