An Observation of the Protective Effects of SAdenosylLMethionine on Liver Function in Cirrhotic Rats after Hepatectomy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Jie ¹ , LIAO Chuanwen ² , ZHU Hong ¹ , TIAN Daguang ¹ , GUO Yongzhang ¹

Department of HepatoBiliary, The Second Hospital Affiliated of Kunming Medical College， Kunming 650101, China;

Corresponding author：

Keywords：

S-adenosylLmethionine; Cirrhosis; Hepatectomy; Liver regeneration; Tumor necrosis factoralpha

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ObjectiveTo investigate the protective effect of SadenosylLmethionine on liver regeneration and liver function in cirrhotic rats after hepatectomy. MethodsCirrhosis was successfully induced by injection of 40% CCl4.Then,partial hepatectomy (about 30%) was performed in all rats. Cirrhotic rats were divided into 3 groups,namely,cirrhotic group (normal saline 5 ml/d,for 15 postoperative days，n=20),treatment group 1 〔S adenosylLmethionine 10 mg/(kg·d),for 15 postoperative days,n=16〕 and treatment group 2 〔SadenosylL methionine 20 mg/(kg·d),15 postoperative days,n=16〕,and normal control group was also established. Animals were sacrificed at the 15th postoperative day and 30th postoperative day to take samples for detection of liver function (Alb,ALT,TB,TBA) and serum TNFα.Liver tissues were also observed under light microscope and electron microscope. ResultsIn two treatment groups,at the time point (15 postoperative days or 30 postoperative days),concentrations of ALT,TB,TBA,Alb and TNFα were decreased significantly as compared with cirrhotic group (P ＜0.01),and concentration of Alb was increased significantly (P＜0.01).In contrast, there were no obvious difference in the same time point of different dosetreatment groups (P gt;0.05),but the decrease of ALT,TB,TBA,TNFα and the increase of Alb were more significant at the second time point (30th postoperative day) than the first time point (15th postoperative day) when treated with same dose (P＜0.01).At the same time,concentration between TNF α and ALT,TB,TBA showed a positive correlation (P＜0.01),and the concentration between TNFα and Alb showed a negative correlation (P＜0.01).In addition, the histopathology showed SadenosylLmethionine had effects of protecting liver function and enhancing liver regeneration. ConclusionThe study suggests that SadenosylL methionine has the efficacy of enhancing liver regeneration and improving liver function.

Citation： ZHANG Jie,LIAO Chuanwen,ZHU Hong,TIAN Daguang,GUO Yongzhang. An Observation of the Protective Effects of SAdenosylLMethionine on Liver Function in Cirrhotic Rats after Hepatectomy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 332-335. doi: Copy

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1. 2Higgins GM. Experimental pathology of liver restoration of the liver of the white rat following partial surgical removal [Ｊ]. Aech Pathol, 1991; 12(2)∶186.
2. 3Almasio P, Pagliaro L. Ademetionine: the state of the art and future prospects [Ｊ]. Ann Ital Med Int， 1993; 8(1 Suppl)∶52S.
3. 4VarelaMoreiras G, AlonsoAperte E, Rubio M,et al. Carbon tetrachlorideinduced hepatic injury is associated with global DNA hypomethylation and homocysteinemia: effect of Sadenosylmethionine treatment [Ｊ]. Hepatology, 1995; 22(4 Pt 1)∶1310.
4. 5Holecek M, Skopec F, Sprongl L. Influence of buthionine sulfoximine, Sadenosylmethionine and glutathione on liver regeneration following partial hepatectomy [Ｊ]. Arzneimittelforschung， 2000; 50(12)∶1093.
5. 6Mato JM, Corrales FJ, Lu SC,et al. SAdenosylmethionine: a control switch that regulates liver function [Ｊ].FASEB J, 2002; 16(1)∶15.
6. 7Feo F,Garcea R,Daino L,et al. Early stimulation of polyamine biosynthesis during promotion by phenobartial of diethylnitrosanineinduced rat liver carcinogenesis. The effect of variations of the SadenosylLmethionine cellular pool [Ｊ].Carcinogenesis,1985; 6(12)∶1713.
7. 8Lieber CS. Role of oxidative stress and antioxidant therapy in alcoholic and nonalcoholicliver diseases [Ｊ]. Adv Pharmacol， 1997; 38(8)∶601.
8. 9Drahota Z, Rauchova H, Sedlak V, et al. The effect of triiodothyronine on changes of membrane fluidity in regenerating rat liver [Ｊ]. Physiol Res， 1999; 48(2)∶167.
9. 11Watson WH,Zhao Y,Chawla RK.Sadenosylmethionine attenuates the lipopolysaccharideinduced expression of the gene for tumour necrosis alpha [Ｊ].Biochem J, 1999; 342(Pt 1)∶21.
10. 孙自勤，王要军，权启镇，等. 肝纤维化大鼠血清有机磷酸酯酶的变化 [Ｊ].新消化病杂志， 1994; 2（4）∶206.
11. 缪明永，蔡在龙，刘鹏飞,等.大鼠在肝再生过程中线粒体氧化磷酸化的调控 [Ｊ]. 中国病理生理杂志，1999; 15(5)∶398.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

An Observation of the Protective Effects of SAdenosylLMethionine on Liver Function in Cirrhotic Rats after Hepatectomy

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