Preventive Effects of Glycine on Rat Sinusoidal Endothelial Cells Injury after Hepatic Warm IschemiaReperfusion_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SHU Ming , XIONG Benjin , FU Huaqun , JIANG Xiaoqiang , ZHOU Shubing

Department of General Surgery, The Second Affiliated Hospital of Jiangxi Medical College, Nanchang 330006, China;

Corresponding author：

Keywords：

LiverIs,chemiareperfusion injury,Glycine,Receptor

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ObjectiveTo investigate the protective effects of glycine on rat sinusoidal endothelial cells (SECs) after hepatic warm ischemiareperfusion and its mechanism.MethodsSeventytwo male SD rats were randomly divided into the normal control,ischemiareperfusion,glycine plus strychnine treated and glycine treated groups. The changes of endothelin (ET),hyaluronic acid (HA),tumor necrosis factorα (TNFα) content and alanine aminotransterase (ALT), superoxide dismutase (SOD) activity as well as morphology of SECs under light microscope were observed at the time point 1,3,24 h after hepatic reperfusion. The effects of glycine on the above parameters were also observed. ResultsThe group using glycine treated, the abnormal changes of all above parameters were improved remarkably (P＜0.01 or P＜0.05). Strychnine can antagonize these effects partly.ConclusionGlycine can prevent the injury to rat SECs after hepatic warm ischemiareperfusion.It most likely acts through glycine receptor on SECs and Kupffer cells.

Citation： SHU Ming,XIONG Benjin,FU Huaqun,JIANG Xiaoqiang,ZHOU Shubing. Preventive Effects of Glycine on Rat Sinusoidal Endothelial Cells Injury after Hepatic Warm IschemiaReperfusion. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 351-354. doi: Copy

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9.	Nishimura Y, Romer LH, Lemasters, et al. Mitochondrial dysfunction and cytoskeletal disruption during chemical hypoxia to cultured rat hepatic sinusoidal endothelial cells: the pH paradox and cytoprotection by glucose, acidotic pH, and glycine [Ｊ]. Hepatology, 1998; 27(4)∶1039.

1. Hayashi H, Chaudry IH, Clemens MG, et al. Hepatic ischemic modals for determining the effects of ATPMgCl2 treatment [Ｊ]. J Surg Res, 1986; 40(2)∶167.
2. 熊成龙，胡虹，陈孝平，等.常温下肝缺血再灌注肝血窦内皮细胞损伤 [Ｊ]. 中华外科杂志, 2000; 38(4)∶297.
3. Gujral JS, Bucci TJ, Farhood A, et al. Mechanism of cell death during warm hepatic ischemiareperfusion in rats: apoptosis or necrosis? [Ｊ]. Hepatology, 2001; 33(2)∶397.
4. Hisama N, Yamaguchi Y, Ishiko T, et al. Kupffer cell production of cytokineinduced neutrophil chemoattractant following ischemia/reperfusion injury in rats [Ｊ]. Hepatology, 1996; 24(5)∶1193.
5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in cultured human endothelium [Ｊ]. J Clin Invest, 1991; 88(3)∶1054.
6. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation [Ｊ]. Surg Today, 1994; 24(8)∶719.
7. Ikejima K, Qu W, Stachlewitz RF, et al. Kupffer cells contain a glycinegated chloride channel [Ｊ].Am J Physiol, 1997; 272(6 Pt 1)∶G1581.
8. Zhang Y, Ikejima K, Honda H, et al.Glycine prevents apoptosis of rat sinusoidal endothelial cells caused by deprivation of vascular endothelial growth factor [Ｊ]. Hepatology, 2000; 32(3)∶542.
9. Nishimura Y, Romer LH, Lemasters, et al. Mitochondrial dysfunction and cytoskeletal disruption during chemical hypoxia to cultured rat hepatic sinusoidal endothelial cells: the pH paradox and cytoprotection by glucose, acidotic pH, and glycine [Ｊ]. Hepatology, 1998; 27(4)∶1039.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Preventive Effects of Glycine on Rat Sinusoidal Endothelial Cells Injury after Hepatic Warm IschemiaReperfusion

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