Effect of Tumor Necrosis Factoralpha Genetransduced Tumor Vaccine in Mice Liver Cancer Implanted Tumors_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LU Yunfei , QIN Xingan , ZENG Jian , LIN Jinling , LIAO Qinghua

Department of Surgery, The First Affiliated Hospital, Guangxi Medical University， Nanning 530021, China;

Corresponding author：

Keywords：

Tumor necrosis factoralpha; Genetransduced tumor vaccine; Liver cancer

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;ObjectiveUsing human tumor necrosis factoralpha (TNFα) genetransduced human liver cancer cell BEL7404 as tumor vaccine, to study the effect of immune rejection to mice liver cancer implanted tumors. MethodsMice were divided into five groups, and were inoculated with TNFα genetransduced BEL7404 cells which irradiated with 60Co (BEL7404TNFCo group), TNFα genetransduced BEL7404 cells (BEL7404TNF group), BEL7404 cells (BEL7404 group), BEL7404 cell irradiated with 60Co (BEL7404Co group) respectively. Normal saline was injected in control group. Then mice liver cancer H22 cells were implanted to each group, the growth of mice liver cancer implanted tumors was observed. The apoptosis index of implanted tumors was detected by TUNEL method.ResultsCompared to BEL7404 group,BEL7404Co group and control group, the tumor vaccine which did not transduce with TNFα gene and the control group, the tumorigenesis rate of liver cancer implanted tumors was reduced, the growth of implanted tumors was inhibited and the apoptosis of implanted tumors was increased in BEL7404TNFCo group,P＜0.01.There was no difference between BEL7404TNFCo group and BEL7404TNF group,P gt;0.05. ConclusionHuman tumor necrosis factoralpha genetransduced human liver cancer cell can be used as tumor vaccine, it has quite b effect of immune rejection to mice liver cancer implanted tumors.

Citation： LU Yunfei,QIN Xingan,ZENG Jian,LIN Jinling,LIAO Qinghua. Effect of Tumor Necrosis Factoralpha Genetransduced Tumor Vaccine in Mice Liver Cancer Implanted Tumors. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 366-369. doi: Copy

1.	Finn OJ. Tumorspecific immune responses and opportunities for tumor vaccines [Ｊ]. Clin Immunol Immunopathol, 1994； 71（3）∶260.
2.	陆云飞，林进令，邱庆明，等. 原发性肝癌基因治疗研究含人TNF基因重组逆转录病毒转移系统的构建与表达 [Ｊ]. 广西医科大学学报， 1997； 14（4）∶1.
3.	邱庆明，陆云飞. 转基因人肝癌细胞株（BEL7404/TNF）的建立及其生物活性的初步研究 [Ｊ]. 广西医科大学学报， 1999； 16（4）∶408.
4.	Wright P, Braun R, Babiuk L, et al. Adenovirusmediated TNFalpha gene transfer induces significant tumor regression in mice [Ｊ]. Cancer Biother Radiopharm, 1999; 14(1)∶49.
5.	Sato Y, Koshita Y, Hirayama M, et al. Augmented antitumor effects of killer cells induced by tumor necrosis factor genetransdued autologous tumor cells from gastrointestinal cancer patients [Ｊ]. Hum Gene Ther, 1996; 7(15)∶1895.
6.	Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [Ｊ]. Int J Cancer, 1995; 63(1)∶130.
7.	陆云飞, 阳杰武, 罗意革, 等. 外源性TNF基因对人肝癌细胞生长的影响 [Ｊ]. 中国肿瘤生物治疗杂志， 1999； 6（3）∶193.

1. Finn OJ. Tumorspecific immune responses and opportunities for tumor vaccines [Ｊ]. Clin Immunol Immunopathol, 1994； 71（3）∶260.
2. 陆云飞，林进令，邱庆明，等. 原发性肝癌基因治疗研究含人TNF基因重组逆转录病毒转移系统的构建与表达 [Ｊ]. 广西医科大学学报， 1997； 14（4）∶1.
3. 邱庆明，陆云飞. 转基因人肝癌细胞株（BEL7404/TNF）的建立及其生物活性的初步研究 [Ｊ]. 广西医科大学学报， 1999； 16（4）∶408.
4. Wright P, Braun R, Babiuk L, et al. Adenovirusmediated TNFalpha gene transfer induces significant tumor regression in mice [Ｊ]. Cancer Biother Radiopharm, 1999; 14(1)∶49.
5. Sato Y, Koshita Y, Hirayama M, et al. Augmented antitumor effects of killer cells induced by tumor necrosis factor genetransdued autologous tumor cells from gastrointestinal cancer patients [Ｊ]. Hum Gene Ther, 1996; 7(15)∶1895.
6. Koshita Y, Lu Y, Fujii S, et al. Efficacy of TNFalpha genetransduced tumor cells in treatment of established in vivo tumor [Ｊ]. Int J Cancer, 1995; 63(1)∶130.
7. 陆云飞, 阳杰武, 罗意革, 等. 外源性TNF基因对人肝癌细胞生长的影响 [Ｊ]. 中国肿瘤生物治疗杂志， 1999； 6（3）∶193.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Tumor Necrosis Factoralpha Genetransduced Tumor Vaccine in Mice Liver Cancer Implanted Tumors

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