Effect of Cholecystectomy on the Induction of Large Intestine Cancer by Dimethylhydrazine in Mice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ANG Bo ¹ , JIANG Yanyong ² , XIAO Yinqi ³ , et al ¹

Department of General Surgery, The 309th Hospital of PLA,Beijing 100091;

Corresponding author：

Keywords：

Cholecystectomy; Large intestine neoplasm; Cell cycel; Large intestine mucosal epitheliumBile acid

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Objective To investigate the effect of cholecystectomy on the induction of large intestine cancer and its mechanism.
Methods After cholecystectomy, the large intestine neoplasm in mice was induced with dimethylhydrazine(DMH).The histogenesis and growth pattern of large intestine cancer were observed.The Sphase fraction (SPF) of epithelium cells in large intestine mucosa was determined using flow cytometry. Before and after operation, the feces of mice were collected to determine the cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA) and lithocholic acid (LCA) concentration by highperformance liquid chromatography.
Results The average number of large intestine neoplasms per mouse,percentage of large intestine adenomas in examined mice and SPF of experimental group were significantly higher than those of control group (P＜0.05). The fecal LCA concentration in postcholecystectomy was significantly higher than that in precholecystectomy and in sham operation group (P=0.00).
Conclusion The large intestine neoplasm of mice induced by DMH is increased and the proliferation of epithelium cell in large intestine mucosa is increased after cholecystectomy. This suggests that cholecystectomy has promoting effect on induction of large intestine neoplasm in mice. The gut may deal with the increased secondary bile acid(LCA) concentration postcholecystectomy.

Citation： ANG Bo,JIANG Yanyong,XIAO Yinqi,et al. Effect of Cholecystectomy on the Induction of Large Intestine Cancer by Dimethylhydrazine in Mice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(1): 19-22. doi: Copy

1.	Alley PG, Lee SP. The increased risk of proximal colonic cancer after cholecystectomy [Ｊ]. Dis Colon Rectum， 1983; 26(8)∶522.
2.	Adami HO, Krusemo UB, Meirik O. Unaltered risk of colorectal cancer within 14-17 years of cholecystectomy: updating of a populationbased cohort study [Ｊ]. Br J Surg， 1987; 74(8)∶675.
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6.	Kuniyasu T, Tanaka T, Shima H,et al. Enhancing effect of cholecystectomy on colon carcinogenesis induced by methylazoxymethanol acetate in hamsters [Ｊ]. Dis Colon Rectum, 1986; 29(8)∶492.
7.	Fazio VW. Current therapy in colon and rectal surgery [M]. Philadelphia: B.C. Decker Incorporated, 1990∶ 227.
8.	PrestonMartin S, Pike MC, Ross RK, et al. Increased cell division as a cause of human cancer [Ｊ]. Cancer Res, 1990; 50(12)∶7415.
9.	Vernick LJ,Kuller LH.Cholecystectomy and right side colon cancer: an epidemiological study [Ｊ].Lancet, 1981; 2(8243)∶381.
10.	Markman M. Cholecystectomy and carcinoma of the colon [Ｊ]. Lancet, 1982; 2(8288)∶47.
11.	Turunen MJ, Kivilaakso EO. Increased risk of colorectal cancer after cholecystectomy [Ｊ]. Ann Surg, 1981; 194(5)∶639.
12.	Nielsen GP, Theldors A, Tulinius H, et al. Cholecystectomy and colorectal carcinoma: a totalpopulation historical prospective study [Ｊ]. Am J Gastroenterol, 1991; 86(10)∶1486.
13.	Ekbom A, Yuen J, Adami HO, et al. Cholecystectomy and colorectal cancer [Ｊ]. Gastroenterology, 1993; 105(1)∶142.
14.	Narisaw T,Magadia NE,Weisburger JH,et al.Promoting effect of bile acids on colon carcinogenesis after intrarectal instillation of NmethylNnitroNnitnosoguanidine in rats [Ｊ]. J Natl Cancer Inst,1974; 53(4)∶1093.
15.	Reddy BS, Narasswa J, Wesburger JH, et al. Brief communication: promoting effect of sodium deoxycholate on colon adenocarcinomas in germ free rats [Ｊ]. J Natl Cancer Inst,1976; 56(2)∶441.
16.	Zusman I, Zimber A. Ultrastructural changes in rat colorectal epithelium and tumors after treatment with NmethylNnitroNnitrosoguanidine and secondary bile acids [Ｊ]. Acta Anat, 1991; 141(1)∶282.
17.	Morvay K,Szentleleki K,Torok G,et al. Effect of change of fecal bile acid excretion achieved by operative procedures on 1,2dimethylhydrazineinduced colon cancer in rats [Ｊ]. Dis Colon Rectum,1989; 32(10)∶860.
18.	Malagelada JR, Go VLW, Summerskill WHJ, et al. Bile acid secretion and biliary bile acid composition altered by cholecystectomy [Ｊ]. Dig Dis Sci, 1973; 18(5)∶455.
19.	Roda E, Aldini R, Mazzella G, et al. Enteroheptic circulating of bile acids after cholecystectomy [Ｊ]. Gut, 1978; 19(5)∶640.
20.	Zuccato E, Ventari M, Leo GD, et al. Role of bile acids and metabolic activity of colonic bacteria in increased risk of colon cancer after cholecystectomy [Ｊ]. Dig Dis Sci, 1993; 38(3)∶514.
21.	Narisawa T, Sano M, Suto M, et al. The correlation between cholecystectomy and fecal bile acids, and large bowel cancer induced with 1,2dimethylhydrazine in mice [Ｊ]. Dis Colon Rectum, 1985; 28(1)∶27.

1. Alley PG, Lee SP. The increased risk of proximal colonic cancer after cholecystectomy [Ｊ]. Dis Colon Rectum， 1983; 26(8)∶522.
2. Adami HO, Krusemo UB, Meirik O. Unaltered risk of colorectal cancer within 14-17 years of cholecystectomy: updating of a populationbased cohort study [Ｊ]. Br J Surg， 1987; 74(8)∶675.
3. 许红兵，萧荫琪，钱志平,等. 胆囊切除术与大肠癌关系的探讨 [Ｊ].中国肛肠病杂志，1989； 9(2)∶25.
4. Lingeman CH, Garner FM. Comparative study of intestinal adenocarcinomas of animals and man [Ｊ]. J Natl Cancer Inst， 1972; 48(2)∶325.
5. Roges AE. Contribution of laboratory animal studies of colon carcinogenesis. In: Masteromarino AJ. Large bowel cancer [M]. New York： Praeger Published, 1985∶ 1-33.
6. Kuniyasu T, Tanaka T, Shima H,et al. Enhancing effect of cholecystectomy on colon carcinogenesis induced by methylazoxymethanol acetate in hamsters [Ｊ]. Dis Colon Rectum, 1986; 29(8)∶492.
7. Fazio VW. Current therapy in colon and rectal surgery [M]. Philadelphia: B.C. Decker Incorporated, 1990∶ 227.
8. PrestonMartin S, Pike MC, Ross RK, et al. Increased cell division as a cause of human cancer [Ｊ]. Cancer Res, 1990; 50(12)∶7415.
9. Vernick LJ,Kuller LH.Cholecystectomy and right side colon cancer: an epidemiological study [Ｊ].Lancet, 1981; 2(8243)∶381.
10. Markman M. Cholecystectomy and carcinoma of the colon [Ｊ]. Lancet, 1982; 2(8288)∶47.
11. Turunen MJ, Kivilaakso EO. Increased risk of colorectal cancer after cholecystectomy [Ｊ]. Ann Surg, 1981; 194(5)∶639.
12. Nielsen GP, Theldors A, Tulinius H, et al. Cholecystectomy and colorectal carcinoma: a totalpopulation historical prospective study [Ｊ]. Am J Gastroenterol, 1991; 86(10)∶1486.
13. Ekbom A, Yuen J, Adami HO, et al. Cholecystectomy and colorectal cancer [Ｊ]. Gastroenterology, 1993; 105(1)∶142.
14. Narisaw T,Magadia NE,Weisburger JH,et al.Promoting effect of bile acids on colon carcinogenesis after intrarectal instillation of NmethylNnitroNnitnosoguanidine in rats [Ｊ]. J Natl Cancer Inst,1974; 53(4)∶1093.
15. Reddy BS, Narasswa J, Wesburger JH, et al. Brief communication: promoting effect of sodium deoxycholate on colon adenocarcinomas in germ free rats [Ｊ]. J Natl Cancer Inst,1976; 56(2)∶441.
16. Zusman I, Zimber A. Ultrastructural changes in rat colorectal epithelium and tumors after treatment with NmethylNnitroNnitrosoguanidine and secondary bile acids [Ｊ]. Acta Anat, 1991; 141(1)∶282.
17. Morvay K,Szentleleki K,Torok G,et al. Effect of change of fecal bile acid excretion achieved by operative procedures on 1,2dimethylhydrazineinduced colon cancer in rats [Ｊ]. Dis Colon Rectum,1989; 32(10)∶860.
18. Malagelada JR, Go VLW, Summerskill WHJ, et al. Bile acid secretion and biliary bile acid composition altered by cholecystectomy [Ｊ]. Dig Dis Sci, 1973; 18(5)∶455.
19. Roda E, Aldini R, Mazzella G, et al. Enteroheptic circulating of bile acids after cholecystectomy [Ｊ]. Gut, 1978; 19(5)∶640.
20. Zuccato E, Ventari M, Leo GD, et al. Role of bile acids and metabolic activity of colonic bacteria in increased risk of colon cancer after cholecystectomy [Ｊ]. Dig Dis Sci, 1993; 38(3)∶514.
21. Narisawa T, Sano M, Suto M, et al. The correlation between cholecystectomy and fecal bile acids, and large bowel cancer induced with 1,2dimethylhydrazine in mice [Ｊ]. Dis Colon Rectum, 1985; 28(1)∶27.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Cholecystectomy on the Induction of Large Intestine Cancer by Dimethylhydrazine in Mice

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