Study on the Expression of Rb Gene in Primary Hepatocellular Carcinoma by Tissue Chip_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Kai ¹

Key Laboratory for Molecular Biology of Infectious Diseases, West China Hospital, Sichuan University,Chengdu 610041;

Corresponding author：

Keywords：

Rb geneHepatocellular carcinomaImmunohistochemistryTissue chip

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Objective To determine whether Rb gene is involved in the genesis of primary hepatocellular carcinoma(HCC). MethodsForty paraffin specimens of primary HCCs with corresponding adjacent liver tissues and normal liver tissues were investigated for Rb protein expression by tissue chip and SP immunohistochemical technique. ResultsLoss of Rb protein expression occurred in 17 of 40 tumor samples, whereas in 4 of 40 adjacent liver tissue samples, only 1 of 40 normal liver tissue specimens showed negative Rb staining.Rb protein deletion in HCC was higher than that in adjacent tissues and normal liver tissues (P＜0.05).Rb protein deletion rate doesn’t correlated remarkably with tumor size or phathology grade of HCC （P gt;0.05）. ConclusionRb protein deletion may play an important role in the tumorigenesis of HCC.Tissue chip is an effective highthroughput technique platform for the study of tumor molecular pathology.

Citation： LIU Kai,HE Xu,ZHAO Lianshan,et al. Study on the Expression of Rb Gene in Primary Hepatocellular Carcinoma by Tissue Chip. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(3): 177-179. doi: Copy

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1. Edmondson HA，Steiner PE. Primary carcinoma of the liver： a study of 100 cases among 48900 necropsies [J]. Cancer,1954; 7(2)∶462.
2. 周思亮, 赵连三. 生物组织标本观察片 [P]. 中国，实用新型专利，ZL 99 2 41752.X.2000.9.9.
3. Friend SH, Bernards R, Rogelj S,et al. A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma [J]. Nature,1986; 323(6089)∶643.
4. Corce CM. Genetic approaches to the study of the molecular basis of human cancer [J]. Cancer Research, 1991; 51(18 Suppl)∶5015.
5. Kononen J,Bubendorf L,Kallioniemi A,et al. Tissue microarrays for highthroughput molecular profiling of tumor specimens [J]. Natural Medicine,1998; 4(7)∶844.
6. Zhang X, Xu HJ, Murakami Y, et al. Deletions of chromsome 13q,mutations in retinoblastoma 1, and retinoblastoma protein state in human hepatocellular carcinoma [J]. Cancer Research,1994; 54(15)∶4177.
7. Hui AM, Li X, Makuuchi M, et al. Overexpression and lack of retinoblastoma protein are associated with tumor progression and metastasis in hepatocellular carcinoma [J]. International J Cancer,1999; 84(6)∶604.
8. Murakami Y,Hagashi K, Hirohashi S, et al. Aberrations of the tumor suppressor gene p53 and retinoblastoma gene on human HCC [J].Cancer Research,1991; 51(20)∶5520.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on the Expression of Rb Gene in Primary Hepatocellular Carcinoma by Tissue Chip

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