The Effects of Newcastle Disease Virus on Tumor Infiltrating Lymphocyte_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Medical Centre of General Surgery, General Hospital of Chengdu Commond, Chengdu 610083;

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Newcastle disease virus Tumor infiltrating lymphocyte Primary hepatic carcinoma

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ObjectiveTo study the effects of newcastle disease virus (NDV) on tumor infiltrating lymphocyte (TIL). MethodsTILs were dissociated from 6 cases of primary hepatic carcinoma. Each case was divided into 3 portions equally, and was added 20 μl NDV with HA titer of 1∶160 (NDV group), rIL2 with the end concentration of 1 000 u/ml (rIL2 group) and 0.9% sodium chloride (control group) respectively, then cultured in the condition of 37℃，5% CO2. CD3, CD4 and CD8 were assayed by flow cytometry. The contents of IL2, IFNγ and TNFβ in supernate of NDV group and control group were examined by ELISA.ResultsCoupled material revealed that CD3 and CD4 were significantly higher in NDV group than in the other two groups (P＜0.05, P＜0.01). CD8 had no significant changes in three groups. The contents of IL2, IFNγ and TNFβ in NDV group were significantly higher than those in control group (P＜0.01, P＜0.01 and P＜0.05 respectively). ConclusionNDV can activate TIL directly.

Citation： ZHAO Tiejun,CHEN Tao,TIAN Fuzhou,et al.. The Effects of Newcastle Disease Virus on Tumor Infiltrating Lymphocyte. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(5): 329-331. doi: Copy

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1. 赵铁军，田伏洲，尹志良，等.新城疫病毒治疗肝癌10例 [J].世界华人消化杂志，1999； 7(6)∶ 718.
2. Schirrmacher V, Haas C, Bonifer R, et al. Viros potentiation of tumor vaccine Tcell stimulatory capacity requires cell surface binding but not infection [J]. Clin Cancer Res, 1997; 3(7)∶1135.
3. Haas C, Ertel C, Gerhards R, et al. Introduction of adhesive and costimulatory immune functions into tumor cells by infection with Newcastle Disease Virus [J]. Int J Oncol,1998; 13(6)∶1105.
4. Kenney S，Pagano JS. Viruses as oncolytic agents: a new age for “therapeutic” viruses？ [J]. J Natl Cancer Inst, 1994; 86(16)∶1185.
5. Yeow WS, Beilharz MW，Lai CM. The in vitro expression patterns of individual type I interferon genes in Newcastle disease virus infected murine splenocytes and fibroblasts [J]. Int J Biochem Cell Biol,1997; 29(3)∶513.
6. Weidmann E, Trucco M， Whiteside TL. Relevance of the T cell receptor for immunotherapy of cancer [J]. Cancer Immunol Immunother, 1994; 39(1)∶1.
7. Kagi D, Seiler P, Pavlovic J, et al. The roles of perforin and Fasdependent cytotoxicity in protection against cytopathic and noncytopathic viruses [J]. Eur J Immunol, 1995; 25(10)∶3256.
8. Bradley LM, Yoshimoto K，Swain SL. The cytokines IL4, IFNgamma, and IL12 regulate the development of subsets of memory effector helper T cells in vitro [J]. J Immunol, 1995; 155(4)∶1713.
9. Noble A, Macary PA，Kemeny DM. IFNgamma and IL4 regulate the growth and differentiation of CD8+ T cells into subpopulations with distinct cytokine profiles [J]. J Immunol, 1995; 155(6)∶2928.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Effects of Newcastle Disease Virus on Tumor Infiltrating Lymphocyte

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