Experimental Research of Recombinant Human Growth Hormone on Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Tong ¹ , JIANG Xiufeng ² , WANG Chen ¹ , et al. ¹

Department of General Surgery, The Second Affiliated Hospital, Lanzhou Medical College, Lanzhou 730030;

Corresponding author：

Keywords：

Obstructive jaundice Recombinant human growth hormone Experimental research

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ObjectiveTherapeutical effect of recombinant human growth hormone （rhGH) on obstructive jaundice and internal and external drainage was observed.MethodsNew Zealand white rabbits were randomly divided into groups below: obstructive jaundice internal drainage plus rhGH group, obstructive jaundice internal drainage plus NS group, obstructive jaundice external drainage plus NS group, and obstructive jaundice external drainage plus rhGH group. After the establishment of obstructive jaundice model, rhGH was used in the above groups. Subcutaneous injection of rhGH 0.2 IU/kg was given twice a day. Isovolume NS was used on the control groups. Full set of endotoxin, tumor necrosis factor, sIL2R and nutritional status were estimated before the model establishment, and 14 days after the model established, 14 days after internal and external drainage.ResultsFour days after internal and external drainage, body weight of therapy groups was increased compared with control groups (P＜0.05). Seven days and ten days after obstructive jaundice, blood sugar of therapy groups rised compared with control groups (P＜0.05). Albuminate, siderophilin and prealbumin of therapy groups were all observed an increase after 14 days after obstructive jaundice, and 14 days after internal and external drainage (P＜0.01). Blood total cholesterol, low density lipoprotein and omni bile acid of therapy groups after 14 days of obstructive jaundice were increased apparently (P＜0.05). Blood glutamicoxal acetic transaminase, transglutaminase, total bilirubin, blood uria nitrogen, creatinine and uric acid of therapy group after 14 obstructive jaundice days were increased (P＜0.05). Ca2+ of therapy groups 14 days after obstructive jaundice, 14 days after internal and external drainage rised as compared with control groups (P＜0.05). However, K+,Na+ of therapy groups 14 days after external drainage decreased (P＜0.05). An increasing tendency of sIL2R was observed in control groups 14 days after obstructive jaundice(P＜0.05) and ET,αTNF,sIL2R of control groups was decreased 14 days after internal and external drainage (P＜0.01).ConclusionAfter rhGH is used in obstructive jaundice and internal and external drainage, an improvement of nutritional status and immunological function can be observed.

Citation： WANG Tong,JIANG Xiufeng,WANG Chen,et al.. Experimental Research of Recombinant Human Growth Hormone on Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(6): 408-411. doi: Copy

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1. 王彤，寇志民，王琛，等. 梗阻性黄疸可重复性体内外引流模型的建立 [J]. 医学研究生学报， 2002; 15(1)∶62.
2. Ellegard L, Bosaeus I, Nordgren S, et al. Lowdose recombinant human growth increases body weight and lean body mass in patients with short bowel syndrome [J]. Ann Surg,1997; 225(1)∶88.
3. Wolf RF, Heslin MJ, Newman E，et al. Growth hormone and insuline combine to improve wholebody and skeletal muscle protein kinetics [J]. Surgery, 1992; 112(2)∶284.
4. Jeevanandam M, Holaday NJ, Petersen SR. Adjuvant recombinant human growth hormone dose not augment endogenous glucose production in total parenteral nutritionfed multiple trauma patients [J]. Metabolism, 1996; 45(4)∶450.
5. 徐德征, 胡铭荣, 王雪根. 梗阻性黄疸时内毒素血症对肾功能的影响及胆酸盐、乳果糖和山莨菪碱的干预 [J]. 中华普通外科杂志, 2001; 16(11)∶675.
6. 顾军, 黎介寿, 李维勤,等. 重组生长激素对严重感染后蛋白质代谢影响的实验研究 [J]. 中华外科杂志, 1997; 35(2)∶104.
7. Knox JB, Demling RH, Wilmore DW, et al. Hypercalcemia associate with the use of human growth hormone in an adult surgical intensive care unit [J]. Arch Surg, 1995; 130(4)∶442.
8. Rubin LA, Nelson DL. The soluble interleukin2 receptor: biology, function, and clinical application [J]. Ann Intern Med, 1990; 113(8)∶619.
9. 李立，冉江华，陆晓青，等. 检测阻塞性黄疸患者手术前后细胞因子的临床意义 [J]. 中国普外基础与临床杂志, 2000; 7(3)∶170.
10. 张延龄. 抗细胞因子疗法在脓毒血症中的试验和临床研究 [J]. 国外医学外科学分册, 1999； 26（1）∶1.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Research of Recombinant Human Growth Hormone on Obstructive Jaundice

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