THE EXPRESSION OF CYCLIN D1、Rb AND p16 PROTEIN IN EARLY GASTRIC CARCINOMA_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Pathology, Second Affiliated Hospital, Xi’an University of Medical Sciences, Xi’an 710004;

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Early gastric carcinoma Cell regulatory factor Immunohistochemistry

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Objective To investigate the expression of cell division regulators p16, Rb and cyclin D1 in human early gasric carcinoma tissues and their role in tumor transformation and the correlation among p16, Rb and cyclin D1. Methods A comparative study was carried out by using immuno-histochemical techniques between the paracarcinomatous intestinal metaplasia of 39 cases of early gatric carcinoma and the non-carcinomatous gastric mucosal intestinal metaplasia tissues of 34 cases.
Results Over expression of cyclin D1 was determined in 33/39 carcinomatous samples(84.6%) and also in para-carcinomatous intestinal metaplasia tissues. p16 was undetectable in 12 of 39 samples. Interestingly, 15 of 26 Rb positive cancers had no or low p16,while 9 Rb negative cancers showed high levels of p16.
Conclusion The over expression of cyclin D1 may be a common molecular abnormality and an early molecular event in early gastric carcinoma. Cyclin D1 over expression and Rb inactivation can co-exist in early gastric carcinoma. However, there is a reciprocity between Rb inactivation and p16 expression in early gastric carcinoma. Thus, abnormality in the negative feedback regulatory pathway of cyclin D1,Rb and p16 may be related to the tumorigenesis in early gastric carcinoma.

Citation： CHEN Xiaoli,WANG Kangmin,SU Baoshan,et al.. THE EXPRESSION OF CYCLIN D1、Rb AND p16 PROTEIN IN EARLY GASTRIC CARCINOMA. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(1): 29-31. doi: Copy

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1. Constancia M, Seruca R, Carneiro F, et al. Retinoblatoma gene structure and product in human gastric carcinoma[J]. Br J Cancer, 1994; 70(5)∶1018.
2. Igaki H, Sasaki H, Tachimori Y, et al. Mutation frequency of the p16/CDKN2 gene in primary cancers in the upper digestive tract[J]. Cancer Res, 1995; 55(15)∶3421.
3. Sakata K, Tamura G, Maesawa C, et al. Loss of heterozygosity on the short arm of chromosome 9 without p16 gene mutation in gastric carcinoma [J]. Jan J Cancer Res, 1995; 86(4)∶333.
4. Akama Y, Yasui W, Kuniyasu H, et al. Genetic status and expression of the cyclin depent kinase inhibitor in human gastric carcinoma cell lines [J]. Jan J Cancer Res, 1996; 87(8)∶824.
5. 吕有勇，高崇峰，崔建涛等.胃癌中MTS1/p16基因缺失及表达异常的研究 [J].中华肿瘤杂志，1996； 18(3)∶442.
6. 邓仲端，陈学诗，彭杰青等.外科病理学 [M].武汉：湖北科技出版社，1978∶87～88.
7. Gillett C, Fant V, Bartek J, et al. Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining [J]. Cancer Res, 1994; 54(9)∶1812.
8. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16 INK4 expression in primary lung cancer and cell lines[J]. Cancer Res, 1995; 55(3)∶505.
9. Sakaguchi M, Fujii, Hirabayashi H, et al. Inversely correlated expression of p16 and Rb protein in nonsmall cell lung cancers:an immunohistochemical study [J]. Int J Cancer, 1996; 65(3)∶442.
10. Yeager T, Studler W, Belair C, et al. Increased p16 levels correlate with Rb alteratious in human urothelial cell [J]. Cancer Res, 1995; 55(3)∶493.
11. 蔺玉昌，黄长铭，刘道坤等.星形细胞瘤p16与Rb蛋白的表达及其相关性研究 [J].中华病理杂志，1997； 26(6)∶340.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE EXPRESSION OF CYCLIN D1、Rb AND p16 PROTEIN IN EARLY GASTRIC CARCINOMA

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