THE INFLUENCE OF GLUCAGON-LIKE PEPTIDE-1 ON GLUCOSE TOLERANCE AFTER HEPATECTOMY_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Hepatectomy Glucagon-like peptide Glucose tolerance

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Objective To investigate the effect of glucagon-like peptide-1(GLP-1) on impaired glucose tolerance due to stress postoperatively.
Methods The rats were allocated randomly to one of three groups, group Ⅰ was subdivided into group Ⅰg which received an intravenous glucose load (0.5 g/kg glucose), and group Ⅰglp which received the same glucose load with GLP-1 (0.3 nmol/kg) during intravenous glucose tolerance test (IVGTT). Rats in group Ⅱg and group Ⅱglp in group Ⅱ were infused respectively the same intravenous glucose tolerance test as group Ⅰ on the first, third and fifth day after 65% liver resection. And rats in group Ⅲ were injected the same glucose load with GLP-1 (0.45 nmol/kg) during IVGTT on the first day after hepatectomy. The peak glucose levels, glucose levels at 30 minutes and the area under the curve (AUC0-30) were investigated among groups.
Results The peak glucose levels, glucose levels at 30 minutes and AUC0-30 were significantly lower in group Ⅰglp than those in group Ⅰg. And the values were significantly higher in group Ⅱg than those in group Ⅰg on the first, third and fifth day after operation. There was no significant difference between group Ⅱglp and group Ⅱg in the peak glucose levels on the first day after liver resection, but the peak glucose levels and AUC0-30 were significantly lower in group Ⅲ than those in group Ⅱg and group Ⅱglp, and the glucose levels at 30 minutes were significantly lower in group Ⅲ than those in group Ⅱg too on the first day. The peak glucose levels were significantly lower in group Ⅱglpthan those in group Ⅱg on the third and fifth postoperative day and in group Ⅱglp on the first day too, and the glucose levels at 30 minutes and AUC0-30 were also significantly lower in group Ⅱglp than those in group Ⅱg, but they were similar between group Ⅱglp and group Ⅰg.
Conclusion Glucose intolerance is a feature of stress after hepatectomy, and GLP-1, injected in conjunction with the IVGTT, increased the clearance of glucose. The contribution of GLP-1 to reducing blood glucose was decreased significantly at early phase postoperatively, but its action was enhanced by the way of dosage dependence. The action of GLP-1 was enhanced with the degree of stress reduction and then returned to normal.

Citation： JIA Qianbing,WANG Jing,ZHAO Jichun,et al.. THE INFLUENCE OF GLUCAGON-LIKE PEPTIDE-1 ON GLUCOSE TOLERANCE AFTER HEPATECTOMY. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(4): 215-217. doi: Copy

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7.	VillanuevaPenacarrillo ML, Alcantara AI, Clemente F, et al. Potent glycogenic effect of GLP1 (7-36) amide in rat skeletal muscle 〔Ｊ〕. Diabetologia, 1994; 37(11)∶1163.
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1. Willms B, Werner J, Holst JJ, et al. Gastric emptying, glucose responses, and insulin secretion after a liquid test meal: effects of exogenous glucagonlike peptide1 (GLP1)-(7-36) amide in type 2 (Noninsulindependent) diabetic patients 〔Ｊ〕. J Clin Endocrinol Metab, 1996; 81(1)∶327.
2. Higgins GM, Anderson RM. Experimental pathology of the liver. I. Restoration of the liver of the white rat following partial surgical removal 〔Ｊ〕. Arch Pathol, 1931; 12∶186.
3. Mori K, Ozawa K, Yamamoto Y, et al. Response of hepatic mitochondrial redox state to oral glucose load 〔Ｊ〕. Ann Surg, 1990; 211(4)∶438.
4. 李晓武，吴言涛. 应激状态下胰岛素抵抗机理的探讨〔Ｊ〕. 中华实验外科杂志， 1992； 9（增刊）∶61.
5. Hvidberg A, Nielsen MT, Hilsted J, et al. Effect of glucagonlike peptide1 (proglucagon 78-107 amide) on hepatic glucose production in healthy man 〔Ｊ〕. Metabolism, 1994; 43(1)∶104.
6. Miki H, Namba M, Nishimura T, et al. Glucagonlike peptide1 (7-36) amide enhances insulin stimulated glucose uptake and decreases intracellular cAMP content in isolated rat adipocytes 〔Ｊ〕. Biochim Biophys Acta, 1996; 1312(2)∶132.
7. VillanuevaPenacarrillo ML, Alcantara AI, Clemente F, et al. Potent glycogenic effect of GLP1 (7-36) amide in rat skeletal muscle 〔Ｊ〕. Diabetologia, 1994; 37(11)∶1163.
8. Ranganath L, Schaper F, Gama R, et al. Effect of glucagon on carbohydratemediated secretion of glucosedependent insulinotropic polypeptide (GIP) and glucagonlike peptide1 (7-36 amide) (GLP1) 〔Ｊ〕. Diabetes Metab Res Rev, 1999; 15(6)∶390.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE INFLUENCE OF GLUCAGON-LIKE PEPTIDE-1 ON GLUCOSE TOLERANCE AFTER HEPATECTOMY

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