EXPERIMENTAL AND CLINICAL STUDY ON IMPROVEMENT OF LIVER FUNCTION AND LIVER REGENERATION BY USING RECOMBINANT GROWTH HORMONE AFTER HEPATECTOMY_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Hepato-Bilio-Pancreatology Surgery, Second Affiliated Hospital, Kuming Medical College, Kunming 650101;

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Recombinant growth hormone Hepatectomy Liver function Liver regeneration

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Objective To study the effect of recombinant growth hormone (rhGH) on improvement of liver function and liver regeneration in animal and patients after hepatectomy.
Methods The liver cirrhosis model of SD species mouse was set up, then the mouse were randomly divided into experiment group and control group, then 30%-40% liver of all the models were resected, rhGH was used by hypodermic injection (0.2-0.4ml/100g) in experimental group, and the equal dose of N.S. were given in control group every day. Then liver function, arterial blood ketone body ratio(AKBR), and the regenerated liver/body weight ratio (RL/W) were determined, histopathology of the cirrhosis with microscope and electron microscope and the mitotic index (MI) of liver cell on 7, 14 and 28th day after operation were observed. Clinically,39 hepatectomized patients were randomly divided into experiment group and control group, liver function, PA， Glu, RI and AKBR were measured preoperatively and on 1, 7,14th day after operation. Postoperative clinical course were also compared between the two groups.
Results In the animal experiment group, as compared with the control group, AKBR was obviously higher (P＜0.01), seruim level of total protein and PA were increased faster (P＜0.05), and RL/W was higher. The mitotic index of liver cell was increased faster on 14th day, the numbers of regenerated liver cell with double nucleus and rough endoplasmic reticulum were higher in 14 and 28th day. In the clinical experiment group, as compared with the control group, serum total bilirubin, alanine aminotransferase and aspartate aminotransferase were lower on 7 and 14th postoperative day (P＜0.05). Serum albumin, PA, Glu, RI and AKBR were higher on 7, 14th postoperative day (P＜0.05).
Conclusion Both experimental and clinical study show that the rhGH can promote liver regeneration and improve liver function after hepatectomy.

Citation： RAN Jianghua,ZHANG Jie,ZHANG Jiahua,et al.. EXPERIMENTAL AND CLINICAL STUDY ON IMPROVEMENT OF LIVER FUNCTION AND LIVER REGENERATION BY USING RECOMBINANT GROWTH HORMONE AFTER HEPATECTOMY. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(4): 223-226. doi: Copy

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1. 吴孟超, 杨广顺. 大鼠肝硬变模型复制的研究〔Ｊ〕. 中华实验外科杂志, 1984; 1(4)∶145.
2. 章宗籍, 欧阳明, 申丽娟等. 人体肝硬变肝细胞含量的研究〔Ｊ〕.昆明医学院学报, 1994; 15(3)∶38.
3. 吴红斌, 严律南. 动脉血酮体比率的测定方法〔Ｊ〕. 普外基础与临床杂志, 1994; 1(2)∶117.
4. Kazue Ozawa著, 严律南译. 氧化还原理论与现代肝脏外科〔M〕. 北京: 中国协和医科大学、北京医科大学联合出版社, 1995∶39~50.
5. 周涌, 吴言涛, 贾乾斌等. rhGH对肝部分切除后促进肝再生的动物实验研究〔C〕. 第五届全国普外基础与临床进展研讨会论文汇编, 1998∶38~39.
6. Wolf M, Ingbar SH, Moses AC. Thyroid hormone and growth hormone interact to regulate insulinlike growth factorI messenger ribonucleic acid and circulating levels in the rat 〔Ｊ〕. Endocrinology, 1989; 125(6)∶2905.
7. Sleim TA, Burns GP, Tropp BE, et al. Hepatic fat accumulation during liver regeneration 〔Ｊ〕. J Surg Res, 1985; 39(4)∶338.
8. Vara Thoreck R, Ruiz requena E, Guerrero JA. Effects of human growth hormon on the catabolic state after surgical trauma 〔Ｊ〕. Hom Res, 1996; 45(1)∶55.
9. 严律南, 曾勇, 文天夫等. 重组生长激素对肝叶切除术后肝脏再生作用的动物实验和临床研究〔Ｊ〕. 中国实用外科杂志, 1999; 19(Suppl)∶46.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EXPERIMENTAL AND CLINICAL STUDY ON IMPROVEMENT OF LIVER FUNCTION AND LIVER REGENERATION BY USING RECOMBINANT GROWTH HORMONE AFTER HEPATECTOMY

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