THE PRODUCTION AND POSSIBLE SIGNIFICANCE OF PLASMA TRYPSINOGEN ACTIVATION PEPTIDES IN EXPERIMENTAL ACUTE PANCREATITIS IN RAT_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

GAO Jun ¹ , ZHANG Shuwen ² , LI Fei. ²

Department of Surgery,Beijing Sixth Hospital, Beijing 100007;

Corresponding author：

Keywords：

Trypsinogen activation peptides Acute pancreatitis Protease inhibitors

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【Abstract】Objective To investigate the production and possible significance of plasma trypsinogen activation peptides (TAP) in rat experimental acute pancreatitis. Methods Ninety SD rats were randomly allocated to five groups: group EP with retrograde ductal infusion of 3%sodium taurocholate; group NP with retrograde ductal infusion of 5%sodium taurocholate; group TP with retrograde ductal infusion of 3%sodium taurocholate and ulinastatin(UTI) intravenous infusion half an hour later; group CP with 0.9% NS retrograde ductal infusion; group OP with sham operation. Animals in each group were killed 3h,6h and 24h after infusion. Plasma TAP was determined by EIA.The histological severity of the pancreas were assessed by Schmidt method. Results The pancreatic pathological changes in group NP was significantly severe than in group EP. At 3h and 6h after infusion, plasma TAP concentration of group NP (4.798±0.169)nmol/L and (3.999±0.299)nmol/L were significant higher than that of group EP(2.416±0.148)nmol/L and (3.356±0.211)nmol/L. At 6h after infusion plasma TAP concentration of group TP 〔(1.611±0.113)nmol/L〕 was significant lower than that of group EP(3.356±0.211)nmol/L. The difference of plasma TAP concentration between group EP and group NP appeared prior to the difference of the histopathological changes of pancreas between two groups. Conclusion Plasma TAP concentration is connected with the severity of sodium taurocholate-induced rat pancreatitis. Plasma TAP concentration may be used as a marker for early assessment of the severity of this experimental acute pancreatitis.

Citation： GAO Jun,ZHANG Shuwen,LI Fei.. THE PRODUCTION AND POSSIBLE SIGNIFICANCE OF PLASMA TRYPSINOGEN ACTIVATION PEPTIDES IN EXPERIMENTAL ACUTE PANCREATITIS IN RAT. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(5): 301-303下转306. doi: Copy

1.	Gudmundsdottir A,Gudmundsdottir E,Oskarsson S,et al. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen 〔Ｊ〕. Eur J Biochem,1993; 217(3)∶1091.
2.	Paul R,Alistair C,Ahmed J, et al. Development of radioimmunoassays for free tetraLaspartylLlysine trypsinogen activation peptides 〔Ｊ〕. J Immunol Meth,1988； 111(2)∶195.
3.	Carlos fernandezdel C,Schmidt J,David W,et al. Generation and possible significance of trypsinogen activation peptide in experimental acute pancreatitis in rat 〔Ｊ〕. Pancreas,1992； 7(3)∶263.
4.	Aho HJ, Koskensalo ML, Nevalainen TJ. Experimental pancreatitisin the rat: sodium taurocholateinduced acute haemorrhagic pancreatitis 〔Ｊ〕. Scand J Gastroenterol, 1980； 15(4)∶411.
5.	Schmidt J, Lewandrowsi K,Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat 〔Ｊ〕. Int J Pancreatol,1992； 12(1)∶41.
6.	Pozsar J, Berger Z, Simon K, et al. Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats 〔Ｊ〕. Pancreas, 1996； 12(2)∶159.
7.	Wang Youxue,Satoru N,Motoji K, et al. Do plasma and urine trypsinogen activation peptides(TAP) really increase in trypsintaurocholateinduced pancreatitis 〔Ｊ〕. Pancreas, 2000； 20(4)∶389.
8.	Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔Ｊ〕. Pancreas, 1995； 10(3)∶306.
9.	Kai M, Casitillo CF,Frick TW, et al. Increased intrapancreatic trypsinogen activation in ischemiainduced experimental pancreatitis 〔Ｊ〕. Ann Surg, 1995； 221(4)∶364.
10.	Simpson KW,Bvmts N,Beechey N, et al. Cholecystokinin8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation 〔Ｊ〕. Dig Dis Sci, 1995； 40(10)∶2152.

1. Gudmundsdottir A,Gudmundsdottir E,Oskarsson S,et al. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen 〔Ｊ〕. Eur J Biochem,1993; 217(3)∶1091.
2. Paul R,Alistair C,Ahmed J, et al. Development of radioimmunoassays for free tetraLaspartylLlysine trypsinogen activation peptides 〔Ｊ〕. J Immunol Meth,1988； 111(2)∶195.
3. Carlos fernandezdel C,Schmidt J,David W,et al. Generation and possible significance of trypsinogen activation peptide in experimental acute pancreatitis in rat 〔Ｊ〕. Pancreas,1992； 7(3)∶263.
4. Aho HJ, Koskensalo ML, Nevalainen TJ. Experimental pancreatitisin the rat: sodium taurocholateinduced acute haemorrhagic pancreatitis 〔Ｊ〕. Scand J Gastroenterol, 1980； 15(4)∶411.
5. Schmidt J, Lewandrowsi K,Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat 〔Ｊ〕. Int J Pancreatol,1992； 12(1)∶41.
6. Pozsar J, Berger Z, Simon K, et al. Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats 〔Ｊ〕. Pancreas, 1996； 12(2)∶159.
7. Wang Youxue,Satoru N,Motoji K, et al. Do plasma and urine trypsinogen activation peptides(TAP) really increase in trypsintaurocholateinduced pancreatitis 〔Ｊ〕. Pancreas, 2000； 20(4)∶389.
8. Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔Ｊ〕. Pancreas, 1995； 10(3)∶306.
9. Kai M, Casitillo CF,Frick TW, et al. Increased intrapancreatic trypsinogen activation in ischemiainduced experimental pancreatitis 〔Ｊ〕. Ann Surg, 1995； 221(4)∶364.
10. Simpson KW,Bvmts N,Beechey N, et al. Cholecystokinin8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation 〔Ｊ〕. Dig Dis Sci, 1995； 40(10)∶2152.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE PRODUCTION AND POSSIBLE SIGNIFICANCE OF PLASMA TRYPSINOGEN ACTIVATION PEPTIDES IN EXPERIMENTAL ACUTE PANCREATITIS IN RAT

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