THERAPEUTIC ROLE OF ANTITNFα ANTIBODY AGAINST ACUTE HEMOERRHAGIC AND NECROTIZING PANCREATITIS IN RATS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Clinical Center of Hepatobiliary Surgery, Southwesten Hospital, 3rd Military Medical University, Chongqing 400038;

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Keywords：

Rat; Acute hemorrhagic and necrotizing pancreatitis; Tumor necrosis factor α monoclonal antibody

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Acute hemorrhagic and necrotizing pancreatitis (AHNP) was induced by injection of sodium taurocholate into pancreatic and biliary duct of rats. TNFα MCAb was infused intravenously 15 minutes before pancreatitis was induced, and plasm TNFα level, serum lipase level and pancratic pathologic changes were tested.Results: the amount of ascites, serum lipase level and palsm TNFα level were significantly incresed and severe pancreatic pathologic changes was induced after AHNP, as compared with those in the control group .However, plasm TNFα level was not elevated after administration of TNFα MCAb, and the amount of ascites and pathologic damage to the pancreas were markely reduced. The animal fatality was reduced too. Conclusions: these suggest that TNFα play an important role in the pathogenesis of AHNP, and TNFα MCAb have a certain therapeutic effect on AHNP in rats.

Citation： Luo Kunlen,He Zhenping,Li Kun,et al.. THERAPEUTIC ROLE OF ANTITNFα ANTIBODY AGAINST ACUTE HEMOERRHAGIC AND NECROTIZING PANCREATITIS IN RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(1): 15-17. doi: Copy

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4.	Grewal HP, Mohey elDin AB, Gaber L, et al. Amelioration of the physiological and biochemical changes of acute pancreatitis using an antiTNF polyclonal antibody. Am J Surg, 1994; 167(1)∶214.
5.	Lorant DE, Patel KP, Mclntyre TM, et al. Coexpression of GMP140 and PAF by endothelium stimulated by histamine of thrombin: a juxtacrine system for adhesion and activation of neutrophils. J Cell Biol, 1991; 115(1)∶223.
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1. Grewal HP, FRCS, Koth M, et al. Induction of tumor necrosis factor in severe acute pancreatitis and it’s subsequent reduction after hepatic passage. Surgery, 1994; 115(2)∶213.
2. Milani R, Pereira MP, Dolhnikoff M, et al. Respiratory mechanics and lung morphometry in severe pancreatitisassociated acute lung injury in rats. Crit Care Med, 1995; 23(11)∶1882.
3. Verduin PA, Punt JMHM, Kreutzer HH. Studies on the determination of lipase activity. Clinica Chimica Acta, 1973; 46(1)∶11.
4. Grewal HP, Mohey elDin AB, Gaber L, et al. Amelioration of the physiological and biochemical changes of acute pancreatitis using an antiTNF polyclonal antibody. Am J Surg, 1994; 167(1)∶214.
5. Lorant DE, Patel KP, Mclntyre TM, et al. Coexpression of GMP140 and PAF by endothelium stimulated by histamine of thrombin: a juxtacrine system for adhesion and activation of neutrophils. J Cell Biol, 1991; 115(1)∶223.
6. Smith CW. Chemotactic factor regulate lectin adhesion molecule1 (LECAMl) dependent neutrophil adhesion to cytokine stimulated endothelium cell in vitro. J Clin Invest, 1991; 87(2)∶609.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THERAPEUTIC ROLE OF ANTITNFα ANTIBODY AGAINST ACUTE HEMOERRHAGIC AND NECROTIZING PANCREATITIS IN RATS

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