THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Luo Chengyu ¹ , Zhu Xueguang ² , Li Shiyong ¹ , et al. ²

Department of General Surgery, Beijing Army General Hospital, Beijing 100700;

Corresponding author：

Keywords：

Colerectal neoplasm; nm23-H1 gene; Loss of heterozygosity; Gene mutation

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

The loss of heterozygosity and mutation for nm23-H1 gene in colorectal carcinomas were studied by Southern blot and RT-PCR-SSCP/silver staining sequencing. The rate of loss of heterozygosity for nm23-H1 was 29.63%. The cases of Duke’s stage D and distant metastatsis had higher frequency of the loss of heterozygosity. No mutation for nm23-H1 was found in colorectal carcinomas. These reaults indicate that the loss of heterozygosity for nm23-H1 may play a significant role in the malignant progression and distant metastasis in colorectal carcinomas.

Citation： Luo Chengyu,Zhu Xueguang,Li Shiyong,et al.. THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(3): 143-145. doi: Copy

1.	Hirayama R, Wawai S, Takagi Y, et al. Positive relationship between expression of antimetastatic factor (nm23 gene productor nucleoside diphosphate kinase) and good prognosis in human breast cancer. J Natl Cancer Inst, 1991; 83(15)∶1249.
2.	Wang L, Patel U, Ghosh L, et al. Mutation in nm23 gene is associated with metastasis in colorectal cancer. Cancer Res, 1993; 53(3)∶717.
3.	骆成玉，祝学光，王申五. 大肠癌中nm23H1表达的定量研究及临床意义. 中华医学遗传学杂志， 1996； 13(5)∶277.
4.	Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: laboratory manuals. 2th ed. New York: Cold Spring Harbor Laboratory Press, 1989∶463-469.
5.	薛涛，祝学光，骆成玉. 应用PCRSSCP银染技术检测结直肠癌p53基因突变. 中华实验外科杂志， 1996； 13(1)∶21.
6.	骆成玉，祝学光. nm23基因的研究近况及其临床应用前景. 国外医学外科学分册， 1995； 22(1)∶24.
7.	Suzuki Y, Orita M, Shiraishi M, et al. Detection of ras gene mutation in human lung cancers by singlestrand conformation polymorphisms analysis of PCR products. Oncogene, 1990; 5(7)∶1037.
8.	Caroline M. Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exon 5 to 8 of the p53 gene. Oncogene, 1994; 9(11)∶1739.
9.	Innis MA, Myambo KB, Gelfand DH, et al. DNA sequencing with thermusaquaticus DNA polymerase and direct sequencing of PCR. Proc Natl Acd Sci USA, 1988; 85(24)∶9436.

1. Hirayama R, Wawai S, Takagi Y, et al. Positive relationship between expression of antimetastatic factor (nm23 gene productor nucleoside diphosphate kinase) and good prognosis in human breast cancer. J Natl Cancer Inst, 1991; 83(15)∶1249.
2. Wang L, Patel U, Ghosh L, et al. Mutation in nm23 gene is associated with metastasis in colorectal cancer. Cancer Res, 1993; 53(3)∶717.
3. 骆成玉，祝学光，王申五. 大肠癌中nm23H1表达的定量研究及临床意义. 中华医学遗传学杂志， 1996； 13(5)∶277.
4. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: laboratory manuals. 2th ed. New York: Cold Spring Harbor Laboratory Press, 1989∶463-469.
5. 薛涛，祝学光，骆成玉. 应用PCRSSCP银染技术检测结直肠癌p53基因突变. 中华实验外科杂志， 1996； 13(1)∶21.
6. 骆成玉，祝学光. nm23基因的研究近况及其临床应用前景. 国外医学外科学分册， 1995； 22(1)∶24.
7. Suzuki Y, Orita M, Shiraishi M, et al. Detection of ras gene mutation in human lung cancers by singlestrand conformation polymorphisms analysis of PCR products. Oncogene, 1990; 5(7)∶1037.
8. Caroline M. Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exon 5 to 8 of the p53 gene. Oncogene, 1994; 9(11)∶1739.
9. Innis MA, Myambo KB, Gelfand DH, et al. DNA sequencing with thermusaquaticus DNA polymerase and direct sequencing of PCR. Proc Natl Acd Sci USA, 1988; 85(24)∶9436.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE RELATION OF THE LOSS OF HETEROZYGOSITY AND MUTATION FOR nm23-H1 WITH THE INHIBITION OF METASTASIS IN COLORECTAL CARCINOMA

Abstract Full text Figures/Tables Video References Cited by

Format

Content