THE RELATIONSHIP BETWEEN THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INITIATION OF CELL CYCLE IN HEPATOMA CELL LINE HEPG2_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Zhang Xiaoshi , Yao Zhenxiang , Tao Xiaohong.

Department of Surgery, College of Clinical Medicine, Chongqing University of Medical Sciences, Chongqing 400016;

Corresponding author：

Keywords：

Vascular endothelial growth factor; Cell cycle; Hepatoma

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

For research the relationship between the expression of vascular endothelial growth factor (VEGF) and the cell proliferation. The expression of VEGF was evaluated while the cell cycle of hepatoma cell line Hep G2, which was synchronized at G0 phase with serum deprivation, and reinitiated with TPA and blocked with antisense oligonucleotides of c-jun. Results: Hep G2 cell did not express VEGF at G0 phase. TPA could induce the expression of VEGF as well as initiation of cell cycle. The antisense oligonucleotides of c-jun could prohibit the expression of VEGF and arrest the cell cycle at G0 phase. Conclusion: The fact that the expression of VEGF accompanies the initiation of cell cycle suggests that they be regulated by the same singnal pathway, the expression of VEGF may be a marker indicating the proliferation of hepatoma cells.

Citation： Zhang Xiaoshi,Yao Zhenxiang,Tao Xiaohong.. THE RELATIONSHIP BETWEEN THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INITIATION OF CELL CYCLE IN HEPATOMA CELL LINE HEPG2. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(6): 342-343. doi: Copy

1.	Mattern J, Koomagi R, Volm M. Association of vascular endothelial growth factor expression with intratumoral microvessel density and tumor cell proliferation in human epidermoid lung carcinomas. Br J Cancer, 1996; 73(7)∶931.
2.	Chow NH, Hsu PI, Lin XZ, et al. Expression of vascular endothelial growth factor in normal and hepatocellular carcinomas: an immunohisto chemical study. Hum pathol, 1997; 28(6)∶698.
3.	Arts J, Grimbergen J, Bosma PJ, et al. Role or cjun and proximal phorbol12myristate13acetate(PMA)responsive elements in the regulation of basal and PMAstimulated plasminogenactivator inhibitor1 gene expression on Hep G2, Eur J Biochem, 1996; 241(2)∶393.
4.	Tischer E, Mitchell R, Hartman T, et al. The human gene for vascular endothelial growth factor. J Biol Chen, 1991; 266(18)∶11947.
5.	Kolch W, MartinyBaron G, Kieser A, et al. Regulation of the expression of the VEGF/VPF and its receptors: role in tumor angiogenesis. Breast Cancer Res Treat, 1995; 36(2)∶139.
6.	Karin M. The regulation of AP1 activity by mitogenactivated protein kinase. J Biol Chem, 1995; 270(28)∶16483.
7.	Yoshida S, Ono M, Shono T, et al. Involvement of interleukin8, vascular endothelial growth factor, and basic fibroblast factor in tumor necrosis factor alphadependent angiogenesis. Mol Cell Biol, 1997; 17(7)∶4015.
8.	Brusselbach S, Mohlesteinlein U, Wang ZQ, et al. Cell proliferation and cell cycle progression are not impaired in fibroblasts and ES cells laking cfos, Oncogene, 1995; 10(1)∶79.

1. Mattern J, Koomagi R, Volm M. Association of vascular endothelial growth factor expression with intratumoral microvessel density and tumor cell proliferation in human epidermoid lung carcinomas. Br J Cancer, 1996; 73(7)∶931.
2. Chow NH, Hsu PI, Lin XZ, et al. Expression of vascular endothelial growth factor in normal and hepatocellular carcinomas: an immunohisto chemical study. Hum pathol, 1997; 28(6)∶698.
3. Arts J, Grimbergen J, Bosma PJ, et al. Role or cjun and proximal phorbol12myristate13acetate(PMA)responsive elements in the regulation of basal and PMAstimulated plasminogenactivator inhibitor1 gene expression on Hep G2, Eur J Biochem, 1996; 241(2)∶393.
4. Tischer E, Mitchell R, Hartman T, et al. The human gene for vascular endothelial growth factor. J Biol Chen, 1991; 266(18)∶11947.
5. Kolch W, MartinyBaron G, Kieser A, et al. Regulation of the expression of the VEGF/VPF and its receptors: role in tumor angiogenesis. Breast Cancer Res Treat, 1995; 36(2)∶139.
6. Karin M. The regulation of AP1 activity by mitogenactivated protein kinase. J Biol Chem, 1995; 270(28)∶16483.
7. Yoshida S, Ono M, Shono T, et al. Involvement of interleukin8, vascular endothelial growth factor, and basic fibroblast factor in tumor necrosis factor alphadependent angiogenesis. Mol Cell Biol, 1997; 17(7)∶4015.
8. Brusselbach S, Mohlesteinlein U, Wang ZQ, et al. Cell proliferation and cell cycle progression are not impaired in fibroblasts and ES cells laking cfos, Oncogene, 1995; 10(1)∶79.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE RELATIONSHIP BETWEEN THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INITIATION OF CELL CYCLE IN HEPATOMA CELL LINE HEPG2

Abstract Full text Figures/Tables Video References Cited by

Format

Content