THE EFFECT OF IMMUNOSUPPRESSION ON INTESTINAL BACTERIA TRANSLOCATION IN EARLY INTESTINAL OBSTRUCTION IN RATS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Surgery, First Affiliated Hospital of Zhejiang Medical University, Hangzhou 310003;

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Keywords：

Bacterial translocation; Intestinal obstruction; Cyclophosphamide

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In order to investigate the mechanism of enterogenous infection, sixty SD rats were randomly allocated into four groups∶ group of intestinal obstruction; group of cyclophosphamide+intestinal obstruction; group of cyclophosphamide and group of sham-operation. Each group included 15 rats. Twenty four hours after obstruction of the terminal ileum, the bacteria in blood of portal vein, blood of heart, peritoneal fluid, mesenteric lymph node (MLN) and content of gut were determined quantitatively, the concentration of endotoxin in portal system were measured. The results showed that early (24 hours) intestinal obstruction led to endotoxemia in portal vein, overgrowth of enteric G－bacili and bacterial translocation into the MLN. The immunosuppressive agent cyclophosphamide not only increased the rate of bacterial translocation into MLN and the number of living bacteria in MLN, but also promoted bacteria to spread into the systemic circulation. The authors conclude that under immunosuppression the bacterial translocation from gut by way of lymphatic channel plays an important role in enterogenous infection.

Citation： Shen Yan,Zheng Shusen,Gu Kezhou,et al.. THE EFFECT OF IMMUNOSUPPRESSION ON INTESTINAL BACTERIA TRANSLOCATION IN EARLY INTESTINAL OBSTRUCTION IN RATS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(1): 9-11. doi: Copy

1.	Deitch EA. The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure. Arch Surg, 1990; 125(3)∶403.
2.	Fry DE. Multiple system organ failure. Surg Clin North Am,1988; 68(1)∶107.
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4.	Marshall JC, Christou NV, Horn R,et al. The microbiology of multiple failure： the proximal gastrointestinal tract as an occult reservoir of pathogens. Arch Surg, 1988; 123(3)∶309.
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6.	Berg RD, Garlington AW. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun,1979; 23(2)∶403.
7.	Deitch EA, Winterton J, Berg R. Thermal injury promotes bacterial translocation from the gastrointestinal tract in mice with impared Tcellmediated immunity. Arch Surg, 1986; 121(1)∶97.
8.	Backer JW, Deitch EA, Li M,et al.Hemorrhagic shock induces bacterial translocation from the gut. J Trauma, 1988; 28(7)∶896.
9.	Navaratnam RL, Morris SE, Traber DL,et al.Endotoxin (LPS) increase mesenteric vascular resistance (MVR) and bacterial translocation (BT). J Trauma, 1990; 30(9)∶1104.
10.	Kobayashi T, Ohmori T, Yanai M,et al.The analysis of the defense mechanism against indigenous bacterial translocation in xirradiated mice. Microbiol Immunol,1991; 35(4)∶315.
11.	Ailverdy JC, Aoys E, Moss GS. Total parenteral nutrition promotes bacterial translocation from the gut. Surgery, 1988; 104(2)∶185.
12.	Deitch EA, Berg RD. Bacterial translocation from the gut： a mechanism of infection. J Burn Care Rehabil, 1987; 8(6)∶475.
13.	Well CL, Maddaus MA, Simmons RL. Role of the macrophage in the translocation of intestinal bacteria. J Trauma, 1987; 122(1)∶48.
14.	Deitch EA, Bridge WM, Ma JW,et al.Obstructed intestine as a reservoir for systemic infection. Am J Surg, 1990; 159(4)∶394.
15.	Mainous MR, Tso T, Berg RD, et al.Studies of the route, magnitude and time course of bacterial translocation in a model of systemic inflammation. Arch Surg, 1991; 126(1)∶33.

1. Deitch EA. The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure. Arch Surg, 1990; 125(3)∶403.
2. Fry DE. Multiple system organ failure. Surg Clin North Am,1988; 68(1)∶107.
3. Carrico CJ,Meakings JL, Marshall JC, et al.Multiple organ failuer syndrome, the gastrointestinal tract： the “Motor” of MOF. Arch Surg, 1986; 121(2)∶196.
4. Marshall JC, Christou NV, Horn R,et al. The microbiology of multiple failure： the proximal gastrointestinal tract as an occult reservoir of pathogens. Arch Surg, 1988; 123(3)∶309.
5. Deitch EA, Winterton J, Li M,et al. The gut as a portal of entry for bacteria. Ann Surg, 1987; 205(6)∶681.
6. Berg RD, Garlington AW. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect Immun,1979; 23(2)∶403.
7. Deitch EA, Winterton J, Berg R. Thermal injury promotes bacterial translocation from the gastrointestinal tract in mice with impared Tcellmediated immunity. Arch Surg, 1986; 121(1)∶97.
8. Backer JW, Deitch EA, Li M,et al.Hemorrhagic shock induces bacterial translocation from the gut. J Trauma, 1988; 28(7)∶896.
9. Navaratnam RL, Morris SE, Traber DL,et al.Endotoxin (LPS) increase mesenteric vascular resistance (MVR) and bacterial translocation (BT). J Trauma, 1990; 30(9)∶1104.
10. Kobayashi T, Ohmori T, Yanai M,et al.The analysis of the defense mechanism against indigenous bacterial translocation in xirradiated mice. Microbiol Immunol,1991; 35(4)∶315.
11. Ailverdy JC, Aoys E, Moss GS. Total parenteral nutrition promotes bacterial translocation from the gut. Surgery, 1988; 104(2)∶185.
12. Deitch EA, Berg RD. Bacterial translocation from the gut： a mechanism of infection. J Burn Care Rehabil, 1987; 8(6)∶475.
13. Well CL, Maddaus MA, Simmons RL. Role of the macrophage in the translocation of intestinal bacteria. J Trauma, 1987; 122(1)∶48.
14. Deitch EA, Bridge WM, Ma JW,et al.Obstructed intestine as a reservoir for systemic infection. Am J Surg, 1990; 159(4)∶394.
15. Mainous MR, Tso T, Berg RD, et al.Studies of the route, magnitude and time course of bacterial translocation in a model of systemic inflammation. Arch Surg, 1991; 126(1)∶33.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE EFFECT OF IMMUNOSUPPRESSION ON INTESTINAL BACTERIA TRANSLOCATION IN EARLY INTESTINAL OBSTRUCTION IN RATS

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