THE EFFECT OF HEPATOCYTE GROWTH PROMOTING FACTOR ON LIVER REGENERATION OF RAT WITH CIRRHOSIS AFTER HEPATECTOMY_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Zhang Jingjie ¹ , Ai Zhongli ² , Jia Guisheng ² , et al. ²

Department of General Surgery, Anhui Province’s Hospital, Hefei 230001;

Corresponding author：

Keywords：

Hepatocyte growth promoting factorCirrhosisHepatectomyLiver regeneration

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In order to observe the effect of hepatocyte growth promoting factor (pHGF) on liver regeneration of rat with cirrhosis after hepatectomy, IBAS Ⅱ auto image analysis technology was used to measure the variety of DNA ploid rate of hepatocytes and OPTDM of enzymes by liver histochemistry after hepatectomy; serum levels of the glutamicpyruvic transaminase (SGPT) and indocyanine green retention rate in 15 minute (ICG15) were tested to measure the function of the remanent liver. The results revealed that tetraploid hepatocytes lowered greatly and diploid, quintploid and ＞quintploid hepatocytes increased apparently in group A. OPTDM of enzymes by liver histochemistry showed no significant difference at the first day after operation in each group (Ｐ＞0.05); SDH and LDH of group A were significantly higher than those of group B and AkP, AcP were significantly lower at the second or fifth day after hepatectomy. Serum tests showed that SGPT, ICG15 of group A decreased apparently at the fifth day after operation. The results demonstrate that pHGF not only stimulates the regeneration of the remanent liver but also accelerates the functional mature of the regenerative hepatocytes and the functional recovery of the remanent liver after resection of cirrhotic liver of rats.

Citation： Zhang Jingjie,Ai Zhongli,Jia Guisheng,et al.. THE EFFECT OF HEPATOCYTE GROWTH PROMOTING FACTOR ON LIVER REGENERATION OF RAT WITH CIRRHOSIS AFTER HEPATECTOMY. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(4): 199-201. doi: Copy

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1. Nakamura T, Nawak, Ichihara A. Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem Biophys Res Commun, 1984; 122(1)∶1450.
2. 吴孟超，杨广顺.大鼠肝硬变模型复制的研究.中华实验外科杂志， 1994； 1(4)∶45.
3. 丛文铭，韦正，吴孟超.用自动图像分析仪测定肝癌细胞核DNA倍体含量.第二军医大学学报，1980； 10(3)∶261.
4. Michalopoulous GK, Zarnegar R. Hepatocyte growth factor. Hepatology, 1992; 15(1)∶149.
5. Kinoshita T, Hiraos, Matsumotok, et al. Possible endocrine control by hepatocyte growth factor of liver regeneration after partial hepatectomy. Biochem Biophys Res Commun, 1991; 177(1)∶330.
6. 汤钊猷主编.原发性肝癌的研究与进展.第1版.上海：上海医科大学出版社， 1990∶7.
7. Tsubouchi H, Niitani Y, Hirno S, et al. Levels of the human hepatocyte growth factor in serum of patients with various liver disease determined by an enzymelinked immunosorbent assay. Hepatology, 1991; 13(1)∶1.
8. 余小航，戴植本.实验性肝硬变大鼠肝部分切除后的再生.同济医科大学学报，1988； 2(3)∶93.
9. 林凌，汤国枝.肝细胞生长因子.细胞生物学杂志， 1994； 16(1)∶15.
10. Shiota G, Rhoads DB, Wang TC, et al. Hepatocyte growth factor inhibits growth of hepatocullular carcinoma cells. Proc Natl Acad Sci USA, 1992; 89(7)∶373.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE EFFECT OF HEPATOCYTE GROWTH PROMOTING FACTOR ON LIVER REGENERATION OF RAT WITH CIRRHOSIS AFTER HEPATECTOMY

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