THE PREPARATION OF IMMUNOTOXIN AND INDUCE OF IMMUNE UNRESPONSIVENESS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Lan Ping , Liu Jialin , Yan Lnan , et al.

Department of Surgery, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

Corresponding author：

Keywords：

CD4; CD8; Immunotoxin; Immune unresponsiveness

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To diminish the specific lymphocytes that responsive to the rejection of allograft. Anti-rat CD4,CD8 monoclonal antibodies and trichosnthin (TCS) was conjugated to immunotoxin by heterobifunctional reagent SPDP, 2-IT. The free TCS was removed from conjugates mixture by a column of Sephacryl S-200. The SDS-PAGE and cytotoxic assay was used to measure the biological activity of immunotoxin. SDS-PAGE showed the immunotoxin, free McAb and TCS were in the mixture of conjugation, and the free TCS can be separated by Sephacryl S-200. In Vitro, the lymphocytes of rat can be killed by antiCD4,antiCD8 immunotoxin. The kill capability was relay to the amount of immunotoxin. The authors consider that the immunology unresponsiveness can be induced by antiCD4,antiCD8 immunotoxin. That was useful in induced transplantation tolerance.

Citation： Lan Ping,Liu Jialin,Yan Lnan,et al.. THE PREPARATION OF IMMUNOTOXIN AND INDUCE OF IMMUNE UNRESPONSIVENESS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(6): 321-323. doi: Copy

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1. Brennan DC, Mohanakumar T, Flye MW. Donorspicific transfusion and donor bone marrow infusion in renal transplantation tolerance, a review of effecacy and mechanisms. Am J Kind Dis, 1995; 26(5)∶701.
2. Cobbold SP, Adams E, Marshall SE, et al. Mechanisms of peripheral tolerance and suppression induced by monoclonal antibodies to CD4 and CD8. Immunol Rev, 1996; 149∶5.
3. Carlsson J, Drevin H, Axen R. Protein thilolation and raversible proteinprotein conjugation. Nsuxxinimidy13(2pyridyldithio) propionate, a new heterobifunctional reagent. Biochem J, 1978; 173(3)∶723.
4. Wang QC. TrichosanthinmAb conjugate sepcifically cytotoxic to human hepatoma cell in vitro. Cancer Res, 1991; 51(12)∶3353.
5. Ellman GL. Tissue sulfhgdryl groups. Arch Biochem Biophy, 1959; 82(1)∶70.
6. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cellmediated cytotoxicity. Science, 1994; 265(5171)∶528.
7. Stalder T, Hahn S, Erb P. Fas antigen is a major targed molecule for CD4＋T cell mediated cytotoxicity. J Immunol, 1994; 152(3)∶1127.
8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol, 1994; 16(2)∶105.
9. Kochi SH, Collier RJ. DNA fragmentation and cytolysis in V937 cell treatment with diphtheria toxin or other inhibitors of protein synthesis. Exp Cell Res, 1993; 208(1)∶296.
10. Waddick KG, Myers DE, Gunther R, et al. In vivo antileukemic activity of B43pokweed antiviral protein against radiation resistant human Bcell procursor leukemia cells. Blood, 1996; 186(11)∶4228.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE PREPARATION OF IMMUNOTOXIN AND INDUCE OF IMMUNE UNRESPONSIVENESS

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